FastQCFastQC Report
Fri 17 Jun 2016
SRR1526750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45719
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1710.3740239287823443No Hit
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.2690347557908091No Hit
TGTCACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.19685469935912858No Hit
TGTCACGGGATTCCTAACCCAAACTTCCAGAAGCTTATAAATTTTACCTCTTACCTGTAGGCCT740.16185830836195017No Hit
TGTCACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.14654738730068462No Hit
TGTCACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.14436011286336098No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC610.1334237406767427No Hit
TGTCACGGGTGGACTTCCTACTATTCCTGTCATGCTGATACTAGGCAAAATGATTCGCTGGGAG590.12904919180209543No Hit
TGTCACGGGGTGGGTGGTGATAAATGGTACTTAGAGATGAGATGTCATAAGGAGATAAATTATT560.12248736849012444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATA150.004504929858.49225656
TTATAAG150.004504929858.49225658
ACGGGTA850.058.170525
ACGGGCA1800.058.1705175
ACGGGAC2500.058.1705175
GGGAATA150.004604551558.1705177
CGGGCAT600.058.1705176
CGGGATT600.058.1705176
CGGGATG1300.058.1705176
GGGATCA258.389701E-658.1705177
TCACGGT201.9611395E-458.1705133
CGGGAAT406.7484507E-1058.1705136
CTTCGTA150.004644856358.0428142
TGTCATA150.004644856358.0428143
TAATAAC150.004644856358.0428150
ACATTAT150.004644856358.0428155
ATGTCAT150.004644856358.0428142
CGTTATG201.9826078E-458.0428139
GTAATAA150.004644856358.0428149
TGTGAGG150.004644856358.0428147
Produced by FastQC (version 0.11.5)