FastQCFastQC Report
Fri 17 Jun 2016
SRR1526749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59384
Sequences flagged as poor quality0
Sequence length64
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5040.8487134581705511No Hit
TGTCACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3400.5725447932102923No Hit
TGTCACGGGGGCGTTCTCTTGGAACGCGCAGCAGAACGAGGTTCTGGTGACCCTAGCCGCGTTC2660.44793210292334634No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1770.2980600835241816No Hit
TGTCACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1570.26438097804122324No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1490.25090933584803987No Hit
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.24754142529974402No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1230.20712649872019398No Hit
TGTCACGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATG1180.19870672234945438No Hit
TGTCACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1100.18523508015627105No Hit
TGTCACGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC1090.18355112488212313No Hit
TGTCACGGGGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACT1080.18186716960797522No Hit
TGTCACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1040.17513134851138354No Hit
TGTCACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1040.17513134851138354No Hit
TGTCACGGGATTCCTAACCCAAACTTCCAGAAGCTTATAAATTTTACCTCTTACCTGTAGGCCT980.16502761686649603No Hit
TGTCACGGGATTCTGTATTGTGTAAGGTTGGAGAGTGAGCCCTTCGTAGTAATAACATTATAAG960.1616597063182002No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC950.1599757510440523No Hit
TGTCACGGGGGATGGGTGTGTGTCTGTCTTGGGATGGGGTGTTCTTGCACCACTTTGTGTTCTT930.15660784049575643No Hit
TGTCACGGGGTCCAGGACACGAAGGCACCTGGCTGAGTTCACTCACGTGGAAGCTGAGTGTCCT900.1515559746733127No Hit
TGTCACGGGGATTTTTAAATAAAACCAAAGAATTACCATGTAATGCAAAGCACACACTTTTTAA870.14650410885086892No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG840.14145224302842516No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA840.14145224302842516No Hit
TGTCACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.14145224302842516No Hit
TGTCACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA800.13471642193183347No Hit
TGTCACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC800.13471642193183347No Hit
TGTCACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG790.13303246665768556No Hit
TGTCACGGGACTGCTGTGGTTGAGCCCTACAATTCCATCCTCACCACCCACACCACCCTGGAGC760.12798060083524182No Hit
TGTCACGGGGTGGCAACCATCAGCTGCCAGGTCAATAAGAGTGACGACTCAGTGATCCAGCTCC760.12798060083524182No Hit
TGTCACGGGGGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTT760.12798060083524182No Hit
TGTCACGGGGTGGGTGGTGATAAATGGTACTTAGAGATGAGATGTCATAAGGAGATAAATTATT760.12798060083524182No Hit
TGTCACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.12461269028694597No Hit
TGTCACGGGATTTCTAAAATTTTTATTTGCAGGGAAGTCTCAGTACTGGCAGTTTCTCTTGGTT730.12292873501279805No Hit
TGTCACGGGGAGCGCCCGGCTGCTGGCCCTGGTGGTTCTGTTCTTTATCGTTGGTGTCATTATA710.11956082446450222No Hit
TGTCACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC710.11956082446450222No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC700.11787686919035431No Hit
TGTCACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG690.1161929139162064No Hit
TGTCACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.11282500336791054No Hit
TGTCACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC670.11282500336791054No Hit
TGTCACGGGTGGACTTCCTACTATTCCTGTCATGCTGATACTAGGCAAAATGATTCGCTGGGAG670.11282500336791054No Hit
TGTCACGGGGGGGTCGGGGGTTCTGGTGGGAAAATGTTCCCCCCATCACTTTTCCTAGTTTTAT630.10608918227131889No Hit
TGTCACGGCAACTGCAGCGAGGAAATGTACCGTCTGATGCTGCAGTGCTGGAAGCAGGAGCCAG620.10440522699717096No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC610.10272127172302303No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCG610.10272127172302303No Hit
TGTCACGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC600.10103731644887512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAAA150.00460132458.20254553
TTAGAAA150.00460132458.20254554
ACATTAT201.9606325E-458.20254555
GTTAGAA150.00460132458.20254553
TTATAAG201.9606325E-458.20254558
ATTATAA201.9606325E-458.20254557
CGCGTTC201.9606325E-458.20254558
GTGATCC201.9688731E-458.15326352
GAGATAA150.004616800758.15326352
AACGTCA150.004632315658.1040649
CAAACGC150.004632315658.1040647
ACAAACG150.004632315658.1040646
TAGCGAG150.004632315658.1040644
CGCTGTG150.004632315658.1040637
TGACGAC150.004632315658.1040642
ATGTCAT150.004632315658.1040642
TCGGCAA150.004632315658.1040650
ATATCTA150.004632315658.1040640
AGTGATC150.004632315658.1040651
TATCTAC150.004632315658.1040641
Produced by FastQC (version 0.11.5)