FastQCFastQC Report
Fri 17 Jun 2016
SRR1526748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60307
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4340.7196511184439617No Hit
TGTCACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3030.5024292370703235No Hit
TGTCACGGGGGCGTTCTCTTGGAACGCGCAGCAGAACGAGGTTCTGGTGACCCTAGCCGCGTTC2160.3581673769214187No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1750.2901819025983717No Hit
TGTCACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1550.2570182565871292No Hit
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.24706916278375646No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1310.2172218813736382No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1220.2022982406685791No Hit
TGTCACGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATG1180.1956655114663306No Hit
TGTCACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1160.19234914686520635No Hit
TGTCACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1080.17908368846070935No Hit
TGTCACGGGGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACT1060.17576732385958513No Hit
TGTCACGGGATTCCTAACCCAAACTTCCAGAAGCTTATAAATTTTACCTCTTACCTGTAGGCCT1040.17245095925846088No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA980.16250186545508813No Hit
TGTCACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC960.15918550085396388No Hit
TGTCACGGGATTCTGTATTGTGTAAGGTTGGAGAGTGAGCCCTTCGTAGTAATAACATTATAAG920.15255277165171538No Hit
TGTCACGGGGGATGGGTGTGTGTCTGTCTTGGGATGGGGTGTTCTTGCACCACTTTGTGTTCTT920.15255277165171538No Hit
TGTCACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG910.15089458935115327No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC870.14426186014890477No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC810.13431276634553205No Hit
TGTCACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.1326545840449699No Hit
TGTCACGGGGGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTT770.12768003714328352No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG770.12768003714328352No Hit
TGTCACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.1260218548427214No Hit
TGTCACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC760.1260218548427214No Hit
TGTCACGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC750.12436367254215928No Hit
TGTCACGGGGATTTTTAAATAAAACCAAAGAATTACCATGTAATGCAAAGCACACACTTTTTAA750.12436367254215928No Hit
TGTCACGGGGAGCGCCCGGCTGCTGGCCCTGGTGGTTCTGTTCTTTATCGTTGGTGTCATTATA720.11938912564047291No Hit
TGTCACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.11938912564047291No Hit
TGTCACGGGGTCCAGGACACGAAGGCACCTGGCTGAGTTCACTCACGTGGAAGCTGAGTGTCCT710.1177309433399108No Hit
TGTCACGGGATTTCTAAAATTTTTATTTGCAGGGAAGTCTCAGTACTGGCAGTTTCTCTTGGTT700.11607276103934867No Hit
TGTCACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC690.11441457873878655No Hit
TGTCACGGGAGTTGGGGGAAGTCGTCTGCTCTCATTTGCGTAATGGTTTCTGTCACTGGTGATT680.11275639643822442No Hit
TGTCACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG670.1110982141376623No Hit
TGTCACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC670.1110982141376623No Hit
TGTCACGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT650.10778184953653806No Hit
TGTCACGGGTGGACTTCCTACTATTCCTGTCATGCTGATACTAGGCAAAATGATTCGCTGGGAG620.10280730263485167No Hit
TGTCACGGGGTGGGTGGTGATAAATGGTACTTAGAGATGAGATGTCATAAGGAGATAAATTATT620.10280730263485167No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACCT150.004512512658.4903343
CATCTGT150.004512512658.4903344
ACAAAGA150.004527576358.44117741
TTCCGCG150.004527576358.44117723
GCGCACG150.004527576358.44117723
TTGGTGA150.004527576358.44117725
TGCCTAC150.004527576358.44117726
TTCGGTA150.004527576358.44117725
TCGGTAG150.004527576358.44117726
CTAGCGC201.9215081E-458.44117723
GGAATTC150.004542676758.39210522
GTTTCCG150.00455781558.3431221
GTTGTTT150.00455781558.3431221
TCCGATA150.0046340758.0994147
CCGATAG150.0046340758.0994148
ACCGAAC150.0046340758.0994151
GCGCAAT150.0046340758.0994147
ATCGTTG150.0046340758.0994147
AGCGGCA150.0046340758.0994146
GTCCGAT150.0046340758.0994146
Produced by FastQC (version 0.11.5)