FastQCFastQC Report
Fri 17 Jun 2016
SRR1526747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56790
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5070.8927628103539356No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3220.5670012326113752No Hit
TGTCACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2510.4419792216939602No Hit
TGTCACGGGGGCGTTCTCTTGGAACGCGCAGCAGAACGAGGTTCTGGTGACCCTAG2070.36450079239302696No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1580.27821799612607856No Hit
TGTCACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1490.2623701355872513No Hit
TGTCACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1480.26060926219404823No Hit
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1370.24123965486881493No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1280.22539179432998768No Hit
TGTCACGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC1260.22187004754358164No Hit
TGTCACGGGATCGGAGGTGGACAGAAGGTCAATGATGACATTATTGTCAACTGGGT1120.19721782003873922No Hit
TGTCACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1040.183130832893115No Hit
TGTCACGGGATTCCTAACCCAAACTTCCAGAAGCTTATAAATTTTACCTCTTACCT1040.183130832893115No Hit
TGTCACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT1040.183130832893115No Hit
TGTCACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.1778482127135059No Hit
TGTCACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG990.17432646592709986No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT990.17432646592709986No Hit
TGTCACGGGGATTTTTAAATAAAACCAAAGAATTACCATGTAATGCAAAGCACACA990.17432646592709986No Hit
TGTCACGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT960.16904384574749076No Hit
TGTCACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC940.16552209896108472No Hit
TGTCACGGGGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAA890.15671773199506955No Hit
TGTCACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.1531959852086635No Hit
TGTCACGGGGACGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGT810.14263074484944532No Hit
TGTCACGGGAGGGTACTGTCTGCTTCTGCCACATACAAGCCATGCACCTTGGGCGC810.14263074484944532No Hit
TGTCACGGGATTCTGTATTGTGTAAGGTTGGAGAGTGAGCCCTTCGTAGTAATAAC800.1408698714562423No Hit
TGTCACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT800.1408698714562423No Hit
TGTCACGGGGAGGGAGAAGCTGTGCTTCCTGGACAAGGTAGAGCCTCAGGCCACCA760.1338263778834302No Hit
TGTCACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT720.12678288431061807No Hit
TGTCACGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT720.12678288431061807No Hit
TGTCACGGGGGATGGGTGTGTGTCTGTCTTGGGATGGGGTGTTCTTGCACCACTTT700.12326113752421201No Hit
TGTCACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC690.12150026413100898No Hit
TGTCACGGGGAACAAGCTGCCCCAGCAGCACACACAGCACAGTGTAAACTGTTTCT680.11973939073780596No Hit
TGTCACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC680.11973939073780596No Hit
TGTCACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG670.11797851734460293No Hit
TGTCACGGGATGGTGGAGGGCGTTGATCCATATGGCCACCTCCTGCCCAGTTAAGG660.1162176439513999No Hit
TGTCACGGGGTCCAGGACACGAAGGCACCTGGCTGAGTTCACTCACGTGGAAGCTG650.11445677055819686No Hit
TGTCACGGGATTTCTAAAATTTTTATTTGCAGGGAAGTCTCAGTACTGGCAGTTTC630.11093502377179082No Hit
TGTCACGGGGATTGCTGAGGCGGCGAGGGAGAGCGTTGGGCTTACCTCACTGCTTT620.10917415037858778No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG610.10741327698538475No Hit
TGTCACGGGAGAGCAGGTGCCATCCTGTGGAAGAACCATGAAGCACTACGAGGTGG590.1038915301989787No Hit
TGTCACGGGGGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTTG590.1038915301989787No Hit
TGTCACGGGGTGGGTGGTGATAAATGGTACTTAGAGATGAGATGTCATAAGGAGAT580.10213065680577567No Hit
TGTCACGGGTGGACTTCCTACTATTCCTGTCATGCTGATACTAGGCAAAATGATTC580.10213065680577567No Hit
TGTCACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT580.10213065680577567No Hit
TGTCACGGGGAGCGCCCGGCTGCTGGCCCTGGTGGTTCTGTTCTTTATCGTTGGTG580.10213065680577567No Hit
TGTCACGGGGAGAGTAATGTGTTGTTTTTTAAAAATGAGCATCTTCTGTATGTATC570.10036978341257263No Hit
TGTCACGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC570.10036978341257263No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT570.10036978341257263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGTC150.00796457450.66159448
GGTCGTC150.00796457450.66159449
CCCAGTT150.00822160650.2566646
TCGATCC150.00822160650.2566645
ACCGTGC150.00822160650.2566646
CCTCCCG150.00822160650.2566645
TCTCGAT150.00822160650.2566643
TTCTCGA150.00822160650.2566642
AATGGTT150.00822160650.2566642
GTGGTCG150.00822160650.2566647
CCGATTG150.00822160650.2566643
CGACCGT150.00822160650.2566644
CGTCTGT150.00822160650.2566642
CTCGATC150.00822160650.2566644
TAATGGT150.00825054150.21206741
TCACCAC150.00825054150.21206741
TGTCGAC150.00825054150.21206741
CTGTCGA150.00830863950.1231240
TGCGTAA150.00833780150.07876237
TGACATT150.00833780150.07876236