FastQCFastQC Report
Fri 17 Jun 2016
SRR1526746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121924
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.3247924936845904No Hit
CCATCTGGGATAGCCTCGTTGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTGAGTTTT2770.22719070896624124No Hit
CCATCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.19110265411239788No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1860.15255405006397427No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1560.127948558118172No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1470.12056691053443129No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.11154489682097045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTAT700.058.067936
CAAATGA150.004655541458.06792453
TCGTGTT150.004663138758.0440347
GTAGGTT201.9997575E-458.02015342
ACAATTA201.9997575E-458.02015343
TATTATA150.004670744858.02015344
ATATTAT150.004670744858.02015343
TACGTAG201.9997575E-458.02015340
CAGTACG202.0079092E-457.9724637
AGTACGT202.0079092E-457.9724638
GTACGTA202.0079092E-457.9724639
GCCCTAT150.004685984557.97245836
GGTCCGA150.004693618557.9486438
GGAAGTA150.004693618557.9486438
TCCAAAC150.004693618557.94864333
CAAGTAT150.004701261457.92484328
TAAACAC150.004701261457.92484329
GCCCTAA303.7672362E-757.92484332
TATAAAG150.004701261457.92484330
CAAGTGA150.004708913657.90106620