FastQCFastQC Report
Fri 17 Jun 2016
SRR1526743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149813
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6870.45857168603525733No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5680.37913932702769454No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5330.3557768684960584No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4350.2903619846074773No Hit
CCATCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG3880.25898954029356597No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2710.1808921789163824No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2580.1722146943189176No Hit
CCATCTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT2550.17021219787334876No Hit
CCATCTGGGACTGCCTTGCGATCCAAAACAATGGCAATTTCTTCTCTCCTCTGTGA2510.16754220261259037No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2480.16553970616702154No Hit
CCATCTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2310.1541922263087983No Hit
CCATCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2270.15152223104803988No Hit
CCATCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2240.1495197346024711No Hit
CCATCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.14818473697209186No Hit
CCATCTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA2170.14484724289614387No Hit
CCATCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2060.13750475592905823No Hit
CCATCTGGGATAGCCTCGTTGTGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAG1960.1308297677771622No Hit
CCATCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1950.1301622689619726No Hit
CCATCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1930.12882727133159338No Hit
CCATCTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA1760.11747979147337013No Hit
CCATCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1640.10946980569109489No Hit
CCATCTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1640.10946980569109489No Hit
CCATCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1620.1081348080607157No Hit
CCATCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1600.10679981043033648No Hit
CCATCTGGGGGCTCCATCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGA1590.10613231161514687No Hit
CCATCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGG1530.10212731872400926No Hit
CCATCTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA1510.10079232109363005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCAA203.8733456E-450.7580949
TACTTTA150.00793044850.75808748
AACTTCG150.00793044850.75808749
ATATATT150.00793044850.75808748
ATAAATC150.00793044850.75808749
ATGACAA204.0255478E-450.36342247
CAATTAT150.00819001650.346444
TTACATG150.00819001650.346444
GATAGGG204.0322717E-450.346444
GATAGGC204.0322717E-450.346444
ACAATTA150.00821192950.3123943
TAGGCTC355.063157E-850.2275841
ACGTAGA204.0795875E-450.2275841
ACGTACT204.0795875E-450.2275841
GTATTAT150.00826690150.22757741
TCACAAT150.00826690150.22757741
TACGTAC204.0863827E-450.2106540
AATTTCT355.1037205E-850.1768336
CAATTTC355.1037205E-850.1768335
TAAAGGC451.3096724E-1050.17682634
Produced by FastQC (version 0.11.5)