FastQCFastQC Report
Fri 17 Jun 2016
SRR1526742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10931
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGATGTGTGAGAAAGCACAGCATTGAGTTATAACTTGAGTTTTTTTCAACATGTGTG1040.9514225596926172No Hit
CGTGGCGGGTCAAAATGTCATTATATACTTGGTGTCTGTGGGAAACTTGTCATAGAAAACCACC610.5580459244350928No Hit
CGTGGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA240.21955905223675784No Hit
CGTGGCGGGAGATAAATGCATTTTTAAATTAATTTTTAGTTATACCTCTACCCTTATGCAAGGT220.20126246455036134No Hit
CGTGGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.16466928917756837No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.16466928917756837No Hit
CGTGGCTGATCGTTCTGTTCAAAAGAAAACGTTAGTTTTAACGGTTAAAAAAAAAAAAAAAAAA150.13722440764797364No Hit
CGTGGCGGGATGTACGAGAGTCGACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.1280761138047754No Hit
CGTGGCGGGCAATCAGGCAGCTGGAAGCTGATATTATGGACATTAATGAAATATTTAAAGACTT140.1280761138047754No Hit
CGTGGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.11892781996157716No Hit
CGTGGCGGGATATTCCAAATTAACTAGGCCTATCTGGTTTCTTTCACTTGAAACTAGAGAATCT130.11892781996157716No Hit
CGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.11892781996157716No Hit
CGTGGCGGGGCATATGTATTCAGAGAGACACAAGTATGTAGAGTCATTCATGGCCCAGAGCGTG120.10977952611837892No Hit
CGTGGCGAGCTGGTTCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10063123227518067No Hit
CGTGGCGCTTCTGCTCCCCGCCCCGAGGACCAGTGCTGGCCGCGCTTCCTGCCTCAGTCCATTG110.10063123227518067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGTG201.8408353E-458.50926256
CATGTGT201.8408353E-458.50926257
GCGGGCT351.4541001E-857.9724775
GCGGGAT1550.057.9724775
TGGTTCT150.004572487457.97247719
TATGCTA150.004572487457.97247741
GTTCTTC150.004572487457.97247721
CTAAGGC150.004572487457.97247745
GGCGAGC258.132705E-657.9724774
GGGAGTC258.132705E-657.9724778
GCGGAGA150.004572487457.9724775
CTGGTTC150.004572487457.97247718
GGGAGCT150.004572487457.9724777
CGTGGCA150.004572487457.9724771
GCTAAGG150.004572487457.97247744
CGGGATC351.4541001E-857.9724776
CGGGAGT258.132705E-657.9724776
CGGGAGA351.4541001E-857.9724776
CGGGACA258.132705E-657.9724776
ATGCTAA150.004572487457.97247742
Produced by FastQC (version 0.11.5)