FastQCFastQC Report
Fri 17 Jun 2016
SRR1526741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15504
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGCTTCTGCTCCCCGCCCCGAGGACCAGTGCTGGCCGCGCTTCCTGCCTCAGTCCATTG1400.9029927760577915No Hit
CGTGGCGGGATGTGTGAGAAAGCACAGCATTGAGTTATAACTTGAGTTTTTTTCAACATGTGTG1180.7610939112487101No Hit
CGTGGCGGGATATTCCAAATTAACTAGGCCTATCTGGTTTCTTTCACTTGAAACTAGAGAATCT870.5611455108359132No Hit
CGTGGCGGGGCATATGTATTCAGAGAGACACAAGTATGTAGAGTCATTCATGGCCCAGAGCGTG870.5611455108359132No Hit
CGTGGCGGGATGCTGCATATCCTGAACCCCGAGGAGCTGCGGGGGATTGAAGAGATTCCCCAGG710.4579463364293086No Hit
CGTGGCGAGCCAGTAGACTGGTTCTTCACTTTACCTACCGTATGCTAAGGCCCGCCCCTCACCC680.43859649122807015No Hit
CGTGGCGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC540.348297213622291No Hit
CGTGGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA510.3289473684210526No Hit
CGTGGCGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATA510.3289473684210526No Hit
CGTGGCGGGACACCACCCTTCCCTTATGGCACTTTAGCCAATTAGTTTAGCTTCCGATTGTGGC490.31604747162022706No Hit
CGTGGCGGGCGCAAGATCTGGTGCTTTGGCCCTGATGGCACTGGCCCCAACATTCTCACCGACA410.2644478844169247No Hit
CGTGGCGGGCAATCAGGCAGCTGGAAGCTGATATTATGGACATTAATGAAATATTTAAAGACTT410.2644478844169247No Hit
CGTGGCGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA380.24509803921568626No Hit
CGTGGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG370.23864809081527347No Hit
CGTGGCGGGCATTTTGCCGTGAGACAGCAGGAGTTAGGCGCGATGCTTTCCACTGCTGCTTACC320.20639834881320948No Hit
CGTGGCGTTCAGTGTCAGACCTCTTAATGGGCCAGTCACTCTTTGACATTCTGTATGCAGTAGT320.20639834881320948No Hit
CGTGGCGGGGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGTGAATCTCT310.19994840041279668No Hit
CGTGGCGGAGGGATGTGGAGGACGAAATCCTATGGGTTGGCGAGAGGATGCCTTTGGCAACTTC310.19994840041279668No Hit
CGTGGCGGGTCAAAATGTCATTATATACTTGGTGTCTGTGGGAAACTTGTCATAGAAAACCACC300.19349845201238391No Hit
CGTGGCGGGATTCGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATGTGTGAGCACCAT300.19349845201238391No Hit
CGTGGCGGAGCTCCCCATTTTCTGAAAGCCATTCAAATACTTGTCAGATTGCTGTGGAAGTCCT300.19349845201238391No Hit
CGTGGCGGGATCTTCGCCTGCGTGGTCACCCACCTGACCCTTCTTTCTCAAGGAAGCAACTTCC280.1805985552115583No Hit
CGTGGCGGGCAACCAGCAGAGTAAAGGCACAGACACAGTTGTGCACCGCATATGTATTCAGAGA280.1805985552115583No Hit
CGTGGCGGGACGTTCAGCTCCTTAGTTCCTTGAACATGGCCATGTCCATCAGGAATAAGAGGAA280.1805985552115583No Hit
CGTGGCGGGGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGGGAATCTCT270.1741486068111455No Hit
CGTGGCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCCC250.1612487100103199No Hit
CGTGGCGGGCAAGTCTCCAAGGGCTCTTCAGCGTCACAAGCCAGCAACTCTCTTTGCATGAAAA230.14834881320949433No Hit
CGTGGCGGGAGCTGGGGCCGTCTGAGCCGCTTACTTAAGCCAGCACTGCTGTGCGGGGCTCTGG230.14834881320949433No Hit
CGTGGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT220.14189886480908154No Hit
CGTGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.13544891640866874No Hit
CGTGGCGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT210.13544891640866874No Hit
CGTGGCGGGAAACTGGTCCTCAGGATCTCAGACTTCCCGCCCCCACTGCACCTGTTTTAAATGA200.12899896800825594No Hit
CGTGGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.12254901960784313No Hit
CGTGGCGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGGCCCAGCAGTGGG190.12254901960784313No Hit
CGTGGCGGGACGTGAAGTGTGTGCATGCAGGCAAACACAGATACACATAAAATTAAAACCCATG190.12254901960784313No Hit
CGTGGCGGGATCCTTCCGGTCATTGGCACAAGAAGCATTGAGAGATGTCCTGTCCTACCACATT190.12254901960784313No Hit
CGTGGCGGGCAGACCCAGATGGACATATGTTTTCCTGCAGAAACTTTAAAGTGACATAAGTAAA180.11609907120743033No Hit
CGTGGCGGGCAGCTGACTAGTATAAGTCCAAAAATGTCCAGTTTGATGCCTGTATTTTAGCTTG180.11609907120743033No Hit
CGTGGCGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTT170.10964912280701754No Hit
CGTGGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC170.10964912280701754No Hit
CGTGGCGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC170.10964912280701754No Hit
CGTGGCGGGGGCTAACAGCTGTTGTCGGTCCAGCAGTGGCTGGGTTTAACTTTCTACTCTTTTT170.10964912280701754No Hit
CGTGGCGGGGGACAGCAGGCTTTAAGAGGGCTTAAATTGGGAGTCAAGGACTATAGTCAGGAGA160.10319917440660474No Hit
CGTGGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG160.10319917440660474No Hit
CGTGGCGGGATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATT160.10319917440660474No Hit
CGTGGCGGGATCTTCGATGTCACAGCTGTTGGGGTTGTAGAAATATTTGTAGATTTCATGAGTA160.10319917440660474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCAC258.274661E-658.00000441
GCGGGGT1200.058.0000045
TGGTTTC258.274661E-658.00000435
AAGTATA150.0046006558.00000418
CAGTCTG150.0046006558.00000436
AAACTAG258.274661E-658.00000451
AATCTCT150.0046006558.00000458
GGGTCAA150.0046006558.0000048
ACAGATA150.0046006558.00000437
GATATTC258.274661E-658.0000049
CGGGGTC501.8189894E-1258.0000046
CCAAATT258.274661E-658.00000415
CCAGTCT150.0046006558.00000435
ACTTGAA258.274661E-658.00000446
TCTGGTT258.274661E-658.00000433
CGGGGAT501.8189894E-1258.0000046
CGGGGAC303.5238918E-758.0000046
TAAAACC150.0046006558.00000454
GAAGTGT150.0046006558.00000414
GTTTCTT258.274661E-658.00000437
Produced by FastQC (version 0.11.5)