FastQCFastQC Report
Fri 17 Jun 2016
SRR1526740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15908
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGGGATGTGTGAGAAAGCACAGCATTGAGTTATAACTTGAGTTTTTTTCAACATGTGTG1290.8109127483027407No Hit
CGTGGCGCTTCTGCTCCCCGCCCCGAGGACCAGTGCTGGCCGCGCTTCCTGCCTCAGTCCATTG1080.6789036962534574No Hit
CGTGGCGGGATGCTGCATATCCTGAACCCCGAGGAGCTGCGGGGGATTGAAGAGATTCCCCAGG880.5531807895398542No Hit
CGTGGCGGGGCATATGTATTCAGAGAGACACAAGTATGTAGAGTCATTCATGGCCCAGAGCGTG650.40859944681921045No Hit
CGTGGCGAGCCAGTAGACTGGTTCTTCACTTTACCTACCGTATGCTAAGGCCCGCCCCTCACCC530.3331657027910485No Hit
CGTGGCGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC520.3268795574553684No Hit
CGTGGCGGGTCAAAATGTCATTATATACTTGGTGTCTGTGGGAAACTTGTCATAGAAAACCACC490.3080211214483279No Hit
CGTGGCGGGATATTCCAAATTAACTAGGCCTATCTGGTTTCTTTCACTTGAAACTAGAGAATCT490.3080211214483279No Hit
CGTGGCGGGACACCACCCTTCCCTTATGGCACTTTAGCCAATTAGTTTAGCTTCCGATTGTGGC480.3017349761126477No Hit
CGTGGCGTTCAGTGTCAGACCTCTTAATGGGCCAGTCACTCTTTGACATTCTGTATGCAGTAGT480.3017349761126477No Hit
CGTGGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA470.29544883077696754No Hit
CGTGGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG360.2263012320844858No Hit
CGTGGCGGGCGCAAGATCTGGTGCTTTGGCCCTGATGGCACTGGCCCCAACATTCTCACCGACA360.2263012320844858No Hit
CGTGGCGGAGCTCCCCATTTTCTGAAAGCCATTCAAATACTTGTCAGATTGCTGTGGAAGTCCT360.2263012320844858No Hit
CGTGGCGGGCATTTTGCCGTGAGACAGCAGGAGTTAGGCGCGATGCTTTCCACTGCTGCTTACC360.2263012320844858No Hit
CGTGGCGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATA350.22001508674880563No Hit
CGTGGCGGGAGATAAATGCATTTTTAAATTAATTTTTAGTTATACCTCTACCCTTATGCAAGGT310.194870505406085No Hit
CGTGGCGGGCAATCAGGCAGCTGGAAGCTGATATTATGGACATTAATGAAATATTTAAAGACTT310.194870505406085No Hit
CGTGGCGGAGGGATGTGGAGGACGAAATCCTATGGGTTGGCGAGAGGATGCCTTTGGCAACTTC310.194870505406085No Hit
CGTGGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.18858436007040483No Hit
CGTGGCGGGATTCGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATGTGTGAGCACCAT280.17601206939904449No Hit
CGTGGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG270.16972592406336434No Hit
CGTGGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT250.15715363339200403No Hit
CGTGGCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCCC240.15086748805632386No Hit
CGTGGCGGGGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGTGAATCTCT220.13829519738496354No Hit
CGTGGCGGGCAAGTCTCCAAGGGCTCTTCAGCGTCACAAGCCAGCAACTCTCTTTGCATGAAAA220.13829519738496354No Hit
CGTGGCGGGAAACTGGTCCTCAGGATCTCAGACTTCCCGCCCCCACTGCACCTGTTTTAAATGA220.13829519738496354No Hit
CGTGGCGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGGCCCAGCAGTGGG210.13200905204928337No Hit
CGTGGCGGGCAACCAGCAGAGTAAAGGCACAGACACAGTTGTGCACCGCATATGTATTCAGAGA210.13200905204928337No Hit
CGTGGCGGGATCTTCGCCTGCGTGGTCACCCACCTGACCCTTCTTTCTCAAGGAAGCAACTTCC200.1257229067136032No Hit
CGTGGCGGGGTCGAAGTCATCAGAGTGTCTCCTGTGTCAAACTCCATCATGCTGTCCACCTCGG190.11943676137792306No Hit
CGTGGCGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA190.11943676137792306No Hit
CGTGGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAATATAAG180.1131506160422429No Hit
CGTGGCGGGCAAAAAGAAGAGTATCCAAACATTTAGACACATGAATGGGTGGCAGCTTGGTTAG180.1131506160422429No Hit
CGTGGCGGGGCCTGCTCTGTGGGTTGGCATGAGATCTGCCCCCATAGAATAGGAAAACCTCCAC170.10686447070656273No Hit
CGTGGCGGGACGTGAAGTGTGTGCATGCAGGCAAACACAGATACACATAAAATTAAAACCCATG170.10686447070656273No Hit
CGTGGCGGGATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATT170.10686447070656273No Hit
CGTGGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG160.10057832537088257No Hit
CGTGGCGGGAGGTAACTTAATTTAACTGGCTTTAGTTTTCTTATTTTTAAGATAGTAAATTTGA160.10057832537088257No Hit
CGTGGCGGGATCAAAGATATCTACAAACGCATGGCTGGTTCTGCCTTTGATTTTGAGAACATGA160.10057832537088257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAT150.004399542758.66025545
CACTTGA150.004399542758.66025545
CAGATTG150.004399542758.66025545
AGTTTTT201.8431722E-458.66025545
CTTTCAC150.004455911558.47284341
ACTTGTC150.004455911558.47284339
ACTTGAG201.8727532E-458.47284340
GGATTGA150.004455911558.47284344
AATTACA150.004455911558.47284344
TTTCACT150.004455911558.47284342
CACTGGC150.004455911558.47284339
GAGTTTT201.8727532E-458.47284344
TCTATCC150.004455911558.47284343
AACTTGA201.8727532E-458.47284339
GTTTAAA150.004455911558.47284339
TCAGATT150.004455911558.47284344
TTCTATC150.004455911558.47284342
GCCGCGC150.004455911558.47284339
TCTTTCA150.004455911558.47284340
CTTCTAT150.004455911558.47284341
Produced by FastQC (version 0.11.5)