SRR1526739_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526739_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28431
Sequences flagged as poor quality	0
Sequence length	56
%GC	59

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTGGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	237	0.8335971298934262	No Hit
CGTGGCGGGATGTGTGAGAAAGCACAGCATTGAGTTATAACTTGAGTTTTTTTCAA	131	0.4607646582955225	No Hit
CGTGGCGCTTCTGCTCCCCGCCCCGAGGACCAGTGCTGGCCGCGCTTCCTGCCTCA	113	0.3974534838732369	No Hit
CGTGGCGGGATGCTGCATATCCTGAACCCCGAGGAGCTGCGGGGGATTGAAGAGAT	105	0.3693151841299989	No Hit
CGTGGCGGGTCAAAATGTCATTATATACTTGGTGTCTGTGGGAAACTTGTCATAGA	90	0.31655587211142766	No Hit
CGTGGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	79	0.2778657099644754	No Hit
CGTGGCGGGACACCACCCTTCCCTTATGGCACTTTAGCCAATTAGTTTAGCTTCCG	72	0.25324469768914215	No Hit
CGTGGCGGGATATTCCAAATTAACTAGGCCTATCTGGTTTCTTTCACTTGAAACTA	71	0.24972741022123737	No Hit
CGTGGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	68	0.23917554781752315	No Hit
CGTGGCGGGGCATATGTATTCAGAGAGACACAAGTATGTAGAGTCATTCATGGCCC	65	0.2286236854138089	No Hit
CGTGGCGTTCAGTGTCAGACCTCTTAATGGGCCAGTCACTCTTTGACATTCTGTAT	62	0.2180718230100946	No Hit
CGTGGCGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC	59	0.20751996060638037	No Hit
CGTGGCGGGTACCTGGTTGATCCTGCCCGTAGCATATGCTTGTCTCAAAGATTAAG	59	0.20751996060638037	No Hit
CGTGGCGGGACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCAT	52	0.18289894833104708	No Hit
CGTGGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	48	0.16882979845942808	No Hit
CGTGGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	47	0.16531251099152336	No Hit
CGTGGCGAGCCAGTAGACTGGTTCTTCACTTTACCTACCGTATGCTAAGGCCCGCC	44	0.15476064858780908	No Hit
CGTGGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC	43	0.15124336111990433	No Hit
CGTGGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	42	0.14772607365199958	No Hit
CGTGGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	40	0.14069149871619008	No Hit
CGTGGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40	0.14069149871619008	No Hit
CGTGGCGGGAGTCCAAAGCGCTGTGCCTGTCTGTACACTTTCCCGAGATGCTGTTC	36	0.12662234884457108	No Hit
CGTGGCGGGATAACAGTGGCCGCCATTTCACATCTGTCAGAGTCCAGAATTCAGAG	36	0.12662234884457108	No Hit
CGTGGCGGGCAAGTCTCCAAGGGCTCTTCAGCGTCACAAGCCAGCAACTCTCTTTG	36	0.12662234884457108	No Hit
CGTGGCGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC	34	0.11958777390876157	No Hit
CGTGGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA	34	0.11958777390876157	No Hit
CGTGGCGGGGTGATAACCTGTCTCAAAACCCTCCTCATCATCTACTCCTTCGTCTT	33	0.11607048644085682	No Hit
CGTGGCGGAGCTCCCCATTTTCTGAAAGCCATTCAAATACTTGTCAGATTGCTGTG	32	0.11255319897295205	No Hit
CGTGGCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC	32	0.11255319897295205	No Hit
CGTGGCGGGATTTGGACCAGAAGGGACACCCTTTGAAGATGGTACTTTTAAACTAG	32	0.11255319897295205	No Hit
CGTGGCGGGAGGTAACTTAATTTAACTGGCTTTAGTTTTCTTATTTTTAAGATAGT	29	0.10200133656923781	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCTCA	15	0.007852804	50.775585	50
TTTTCAA	25	1.8578512E-5	50.77558	50
ACTATCA	15	0.007908833	50.684586	48
ACTGACA	15	0.008135929	50.323845	43
ACTCTCC	15	0.008135929	50.323845	42
TAGTCTC	15	0.008135929	50.323845	44
TTCTCAC	15	0.008135929	50.323845	43
ATTGAAG	15	0.008135929	50.323845	46
CACTATC	15	0.008135929	50.323845	47
TCACTAT	15	0.008135929	50.323845	46
CTTGAGT	20	3.9850673E-4	50.323845	41
AGTCTCA	15	0.008135929	50.323845	45
AGTTTTT	20	3.9850673E-4	50.323845	45
CGCGCTT	20	3.9850673E-4	50.323845	41
TTAGTCT	15	0.008135929	50.323845	43
TCTTAGT	15	0.008135929	50.323845	41
GCTAGGC	15	0.008193452	50.23446	39
CTTTAGA	15	0.008251278	50.14539	37
TAGCCTT	15	0.008251278	50.14539	38
ATAGCCT	15	0.008251278	50.14539	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content