FastQCFastQC Report
Fri 17 Jun 2016
SRR1526738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42949
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3100.7217863046869543No Hit
ATCTCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.22584926307946634No Hit
ATCTCTGGGATAATGAAGAACTACAAGTTAGGTGGTTTAAGCAAACAAAAACATCCCTGTCGCA730.169969033039186No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC730.169969033039186No Hit
ATCTCTGGGGTTTCCTTTCTCTCTGGAATGGAGGGAGATACCTGACACAGTTCCCTCACCAAAA680.15832731844746095No Hit
ATCTCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.15134228969242589No Hit
ATCTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC570.1327155463456658No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC550.12805886050897577No Hit
ATCTCTGGGAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCT540.12573051759063075No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC490.11408880299890568No Hit
ATCTCTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG480.11176046008056066No Hit
ATCTCTGGGTATCGTCCGGGCTGTGTCCTTATTTAATTTTTAATTTTTAATTTTTTCGTAGAAT480.11176046008056066No Hit
ATCTCTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.10011874548883559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGG201.9135083E-458.4517757
CAGTCCC150.0045154458.45176357
CCTGTCG150.0045154458.45176356
AGTCCCT150.0045154458.45176358
GGGTAAT150.004621703658.1099437
TCTGGAT150.004621703658.1099434
CTGGATT150.004621703658.1099435
TGGATTG150.004621703658.1099436
GGGATTA150.004621703658.1099437
TGGGATG452.910383E-1158.1099436
TGGGACT452.910383E-1158.1099436
TGGGACA550.058.1099436
GGGAATG258.433093E-658.109947
GGACAGT201.9813716E-458.0420578
GGTTGGG201.992866E-457.97433512
CTATAGC201.992866E-457.97433513
CCTATAG201.992866E-457.97433512
TAAGCAA201.992866E-457.97433538
TTGGGGA201.992866E-457.97433514
TTAAGCA201.992866E-457.97433537
Produced by FastQC (version 0.11.5)