FastQCFastQC Report
Fri 17 Jun 2016
SRR1526737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59117
Sequences flagged as poor quality0
Sequence length64
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6651.1248879340967912No Hit
ATCTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3130.5294585313869107No Hit
ATCTCTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2680.4533382952450226No Hit
ATCTCTGGGGTTTCCTTTCTCTCTGGAATGGAGGGAGATACCTGACACAGTTCCCTCACCAAAA2000.33831216063061387No Hit
ATCTCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.31124718778016475No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1560.2638834852918788No Hit
ATCTCTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1470.2486594380635012No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1310.22159446521305207No Hit
ATCTCTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT1300.219902904409899No Hit
ATCTCTGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1200.20298729637836832No Hit
ATCTCTGGGGGGCTCTTCCGGGGCCTGACCCCCCGCCTTATGTCCAACGCCTTGTCCACTGTGA1150.19452949236260297No Hit
ATCTCTGGGATAATGAAGAACTACAAGTTAGGTGGTTTAAGCAAACAAAAACATCCCTGTCGCA1130.1911463707562968No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1050.17761388433107228No Hit
ATCTCTGGGGTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCTCTTCCAGAGGCCC990.16746451951215388No Hit
ATCTCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.16238983710269464No Hit
ATCTCTGGGAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGTGAATCTCTCTT940.1590067154963885No Hit
ATCTCTGGGAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCT910.15393203308692932No Hit
ATCTCTGGGATTCAATGTGCCTTTTTTTAGGTTTGCTTTTATCTTTGATAAATTGTAAAATCAC860.14547422907116397No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC860.14547422907116397No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA850.1437826682680109No Hit
ATCTCTGGGGATCCAGGCACCTTTGATAGGTAACTACTCCTCTTCCTGTGTTCACACCCACTCT790.13363330344909247No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC770.13025018184278633No Hit
ATCTCTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT750.1268670602364802No Hit
ATCTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG740.12517549943332712No Hit
ATCTCTGGGCAGTGCTATCTCTGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTT740.12517549943332712No Hit
ATCTCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA720.12179237782702099No Hit
ATCTCTGGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAA720.12179237782702099No Hit
ATCTCTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT710.12010081702386792No Hit
ATCTCTGGGATTACAGATGGTTGTGAGCCATCATGTGGTTGCTGGGATTTGAACTCATGACCTT710.12010081702386792No Hit
ATCTCTGGGTAGCTTTGGTCACCTTTTAACTCTGTCCTTGATGTCCCTGTTCCAACAACGCCAC680.11502613461440872No Hit
ATCTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG680.11502613461440872No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT660.11164301300810256No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA650.1099514522049495No Hit
ATCTCTGGGGAGGTCTTAAGCCGCGGCTTCTCTGAGGGGTAGACAGCTGGGCATGGGCCTCCCG640.10825989140179643No Hit
ATCTCTGGGTGCCTGGTGTGCTGGCTCCTGCCTCTGAGGACTACGAGAGGAGGTCGCAGCAAGG640.10825989140179643No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT620.1048767697954903No Hit
ATCTCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA610.10318520899233724No Hit
ATCTCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG600.10149364818918416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAAC201.9775689E-458.1011146
AACATCC201.9775689E-458.1011150
AAACATC201.9775689E-458.1011149
TGTCGCA201.9775689E-458.1011158
ATCTGTT201.9775689E-458.1011144
AGGTCCT201.9775689E-458.1011158
TTTAGAT150.00463315258.10110544
CTCACAA150.00463315258.10110545
CTATCCA150.00463315258.10110554
CTTTTAG150.00463315258.10110542
TGTGGAC150.00463315258.10110552
CAACCAA150.00463315258.10110549
AAGCGCA150.00463315258.10110551
TAGCAAG150.00463315258.10110547
TGACATT150.00463315258.10110556
AACGCCA150.00463315258.10110557
GTCCAAC150.00463315258.10110542
TATGTTG150.00463315258.10110544
ATGTTGC150.00463315258.10110545
GCGTCAT150.00463315258.10110554
Produced by FastQC (version 0.11.5)