FastQCFastQC Report
Fri 17 Jun 2016
SRR1526736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58960
Sequences flagged as poor quality0
Sequence length64
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6711.1380597014925373No Hit
ATCTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2860.4850746268656716No Hit
ATCTCTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2580.437584803256445No Hit
ATCTCTGGGGTTTCCTTTCTCTCTGGAATGGAGGGAGATACCTGACACAGTTCCCTCACCAAAA2030.3443012211668928No Hit
ATCTCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.26458616010854813No Hit
ATCTCTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1390.235753052917232No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1350.2289687924016282No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1270.21540027137042064No Hit
ATCTCTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCAT1200.20352781546811397No Hit
ATCTCTGGGGTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCTCTTCCAGAGGCCC1190.20183175033921305No Hit
ATCTCTGGGGGGCTCTTCCGGGGCCTGACCCCCCGCCTTATGTCCAACGCCTTGTCCACTGTGA1060.1797829036635007No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1040.17639077340569878No Hit
ATCTCTGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1030.1746947082767978No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC990.16791044776119401No Hit
ATCTCTGGGATAATGAAGAACTACAAGTTAGGTGGTTTAAGCAAACAAAAACATCCCTGTCGCA990.16791044776119401No Hit
ATCTCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.16282225237449116No Hit
ATCTCTGGGAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCT870.14755766621438263No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC850.14416553595658071No Hit
ATCTCTGGGAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGTGAATCTCTCTT840.1424694708276798No Hit
ATCTCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA830.14077340569877883No Hit
ATCTCTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT830.14077340569877883No Hit
ATCTCTGGGATTCAATGTGCCTTTTTTTAGGTTTGCTTTTATCTTTGATAAATTGTAAAATCAC810.13738127544097695No Hit
ATCTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG780.13229308005427406No Hit
ATCTCTGGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAA750.12720488466757124No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC750.12720488466757124No Hit
ATCTCTGGGGATCCAGGCACCTTTGATAGGTAACTACTCCTCTTCCTGTGTTCACACCCACTCT740.12550881953867027No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT740.12550881953867027No Hit
ATCTCTGGGTAATGAATTTGATTAGATATATGTGTCCAATGAATTCCGTAATTATCTTTTCATC730.12381275440976934No Hit
ATCTCTGGGCAGTGCTATCTCTGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTT720.12211668928086838No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC670.11363636363636363No Hit
ATCTCTGGGATTACAGATGGTTGTGAGCCATCATGTGGTTGCTGGGATTTGAACTCATGACCTT650.11024423337856173No Hit
ATCTCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA640.10854816824966079No Hit
ATCTCTGGGAGCGTTAGATATCCGCCCTCTAAGCTATTGGTATCTACTTCCCTATCAAATCCCA640.10854816824966079No Hit
ATCTCTGGGTGCCTGGTGTGCTGGCTCCTGCCTCTGAGGACTACGAGAGGAGGTCGCAGCAAGG640.10854816824966079No Hit
ATCTCTGGATTGACGAAACCAGAAATAGCCTGTACCAATACTTCCTTTCCTACTTACAGTCCCT640.10854816824966079No Hit
ATCTCTGGGGATGCTCAAGTGCCACGTGTTTCGCTGTGAGGCACCTGCCAAGAACATCGCCACC630.10685210312075984No Hit
ATCTCTGGGGATATCTGTGGTTAACAGTGCTATTGAGTGGTATGGAACAGTGTTACTTGGCATT620.10515603799185888No Hit
ATCTCTGGGAGCTCTAAGAGCCATTCTGGGCAAGTGAAGGGCAGTGGCTAACAGCTGTTGTCGG620.10515603799185888No Hit
ATCTCTGGGTAGCTTTGGTCACCTTTTAACTCTGTCCTTGATGTCCCTGTTCCAACAACGCCAC610.10345997286295794No Hit
ATCTCTGGGGAGGTCTTAAGCCGCGGCTTCTCTGAGGGGTAGACAGCTGGGCATGGGCCTCCCG610.10345997286295794No Hit
ATCTCTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG610.10345997286295794No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA600.10176390773405698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAA150.004526564458.4429245
ATACATG201.9208877E-458.4429245
TCTATGG150.004526564458.4429245
GTTCGCA150.004541986658.39279644
TAATACA201.937272E-458.34276243
ATAGGTA201.937272E-458.34276226
ATGGAAC201.937272E-458.34276242
TATGGAA201.937272E-458.34276241
TGGAACA201.937272E-458.34276243
GTATGGA201.937272E-458.34276240
TCCAACG201.937272E-458.34276243
GTCCAAC201.937272E-458.34276242
GCTAATA201.937272E-458.34276241
TATGTCC201.937272E-458.34276239
CTAATAC201.937272E-458.34276242
AAATCTG150.00455744858.3427625
CCCTCTA150.00455744858.3427625
CCCTCAA150.00455744858.3427641
ATCTACT150.00455744858.3427642
TGGCGAA150.00455744858.3427641
Produced by FastQC (version 0.11.5)