FastQCFastQC Report
Fri 17 Jun 2016
SRR1526735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52487
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG7081.3489054432526149No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2580.49155028864290207No Hit
ATCTCTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2370.4515403814277821No Hit
ATCTCTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2120.4039095395050203No Hit
ATCTCTGGGGTTTCCTTTCTCTCTGGAATGGAGGGAGATACCTGACACAGTTCCCT1770.3372263608131537No Hit
ATCTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1740.33151065978242233No Hit
ATCTCTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1570.29912168727494426No Hit
ATCTCTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1380.2629222474136453No Hit
ATCTCTGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1150.2191018728447044No Hit
ATCTCTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1130.21529140549088346No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1060.20195476975251014No Hit
ATCTCTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC1050.2000495360755997No Hit
ATCTCTGGGGGGCTCTTCCGGGGCCTGACCCCCCGCCTTATGTCCAACGCCTTGTC980.18671290033722635No Hit
ATCTCTGGGAGCTCAAGTGCCTTGTGGAGGACTTGGGTTCCGTAGCCCATTTGGTT910.17337626459885305No Hit
ATCTCTGGGAGCGTTAGATATCCGCCCTCTAAGCTATTGGTATCTACTTCCCTATC850.1619448625373902No Hit
ATCTCTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA820.15622916150665878No Hit
ATCTCTGGGATAATGAAGAACTACAAGTTAGGTGGTTTAAGCAAACAAAAACATCC810.15432392782974833No Hit
ATCTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT810.15432392782974833No Hit
ATCTCTGGGAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCG790.15051346047592737No Hit
ATCTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT780.1486082267990169No Hit
ATCTCTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC730.13908205841446453No Hit
ATCTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC730.13908205841446453No Hit
ATCTCTGGGGTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCTCTTCC720.13717682473755405No Hit
ATCTCTGGGCAGTGCTATCTCTGGGAGCATTGCTTCTGTGTAAATTATGTACTGCA680.12955589002991216No Hit
ATCTCTGGGATTCAATGTGCCTTTTTTTAGGTTTGCTTTTATCTTTGATAAATTGT670.12765065635300168No Hit
ATCTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA670.12765065635300168No Hit
ATCTCTGGGTAGCTTTGGTCACCTTTTAACTCTGTCCTTGATGTCCCTGTTCCAAC640.12193495532227026No Hit
ATCTCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC600.11431402061462838No Hit
ATCTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG580.11050355326080745No Hit
ATCTCTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG570.10859831958389696No Hit
ATCTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT570.10859831958389696No Hit
ATCTCTGGGAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGTGAA570.10859831958389696No Hit
ATCTCTGGGAGTTTTGCTGTTGTTTCTCAACAGACTGGTATTGATGTTTCCGTTAA560.10669308590698649No Hit
ATCTCTGGGTAATGAATTTGATTAGATATATGTGTCCAATGAATTCCGTAATTATC560.10669308590698649No Hit
ATCTCTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG560.10669308590698649No Hit
ATCTCTGGGGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTACCATGAGT540.10288261855316554No Hit
ATCTCTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA530.10097738487625507No Hit
ATCTCTGGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACG530.10097738487625507No Hit
ATCTCTGGGGTGTGGGCTGGTTTAACCCCGCTTTCCAGTCACTTTTAGATATTCTA530.10097738487625507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTTA150.008016250.573550
CAAGCGC251.9253031E-550.573550
TCCGTTA150.00804693250.52463549
GTTCGCG203.9385154E-450.5246348
CCAATAC150.00829595450.13710435
CATTGAC150.00829595450.13710442
TTTCCTA150.00829595450.13710446
GCTAGGC150.00829595450.13710439
GATACCT150.00829595450.13710437
ATTGGTA150.00829595450.13710436
GTATCTA150.00829595450.13710440
ACCAATA150.00829595450.13710434
GTATTGA150.00829595450.13710438
TCTACTT150.00829595450.13710443
TATTGGT150.00829595450.13710435
CAATACT150.00829595450.13710436
ATCAGAC204.091321E-450.13710434
CACCTGA150.00829595450.13710441
TATCTAC150.00829595450.13710441
AGGCTAG150.00829595450.13710437
Produced by FastQC (version 0.11.5)