FastQCFastQC Report
Fri 17 Jun 2016
SRR1526734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109264
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.1372821789427442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATATA150.00463639358.12425252
ATTAAGC201.9811765E-458.1242551
AGCGCAA201.9811765E-458.1242552
TCGTTCG150.00464485158.0975246
CGTTCGC150.00464485158.0975247
ACTACCG150.00464485158.0975246
TCGCGCA150.00464485158.0975250
ATTACAT201.9856912E-458.0975148
CTATTAC201.9856912E-458.0975146
ACACCGA150.004653320658.07080540
TAAACCG150.004653320658.07080542
AAGCGAA150.004653320658.07080545
CCTAAAG258.555069E-658.07080542
CGTAGAG150.004653320658.07080542
AAACCGT150.004653320658.07080543
TGGTCTC150.004653320658.07080541
TAACTTG150.004653320658.07080545
GTACGTA150.004653320658.07080539
TACGTAG150.004653320658.07080540
TATATTC303.7103746E-758.04411736
Produced by FastQC (version 0.11.5)