FastQCFastQC Report
Fri 17 Jun 2016
SRR1526733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170555
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3280.19231333001084694No Hit
ATGGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2280.1336812172026619No Hit
ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.10084723403007828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT150.00461406258.20937758
CGCAAGC150.00463565658.14079748
ATATTGG150.00463565658.14079748
ACGTATT201.9815814E-458.14079349
TCGTTCG201.9873616E-458.1065646
GCCCGTT201.9873616E-458.1065646
CGTCATC150.004657325358.07237642
CGTAACA201.9931552E-458.07237243
CCAACGA201.9931552E-458.07237241
CTTGTCG150.00466275458.05529440
AGTCACG150.00466275458.05529437
CTATCGG150.00466818858.03822335
CTGTCGT150.0046790758.00411230
TCGTACT150.0046790758.00411233
TGTCGTA150.0046790758.00411231
TCTATCG150.0046790758.00411234
CTGTACG202.0164635E-457.93601625
GGTAAAG150.0047008957.93601626
ACGTCCC150.0047008957.93601624
TACCGTT150.0047008957.93601624
Produced by FastQC (version 0.11.5)