FastQCFastQC Report
Fri 17 Jun 2016
SRR1526732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172234
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2870.1666337656908624No Hit
ATGGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2190.12715259472578005No Hit
ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.10044474377881255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG150.00453653258.45916444
CATAGAC150.00455238858.40769242
TGACGTA150.00456828658.35630426
CGAGTGA150.004573594358.33919524
CGTAAAC150.004573594358.33919521
TTACGCC150.00472410257.86420432
TTGCATT150.00472410257.86420433
TACGTAG150.00472410257.86420429
TTACGTA150.004729544757.8473828
GGTGGAC303.8269172E-757.813778
CGGGATA150.004740443557.813775
CGGGACG303.8269172E-757.813775
TGCGTCG150.004745957.79697819
AGTCCGA150.004751361457.78019710
GAGTCCG150.004751361457.7801979
CTCGGTA150.004751361457.78019716
CGGGGTA2750.056.7626085
TGGCGGG166250.056.666192
ATGGCGG171000.056.4961931
GCGGGGA67750.056.4910934
Produced by FastQC (version 0.11.5)