FastQCFastQC Report
Fri 17 Jun 2016
SRR1526731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284523
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC17510.6154159769157502No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCC7670.26957398874607674No Hit
ATGGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4150.1458581555796895No Hit
ATGGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3960.13918031231218564No Hit
ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.10649402684492994No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2900.10192497618821676No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACA150.00825147150.2631347
GCGTAGA252.049612E-550.13858438
GTGAATC204.1688417E-450.02348729
ACCTCGT301.0429485E-650.01465626
GATACGG150.00855061349.8123820
CGGGGAT32600.049.336635
TGGCGGG281700.048.7519532
ATGGCGG285850.048.697561
GCGGGGA116400.048.618444
GGCGGGG273400.047.8278433
CGGGGAC20200.047.699545
GGCGGGA10650.047.457083
GCGGGGT16100.047.1659434
CGGGGGA28150.047.14155
CGGGATA700.046.238095
CGGGGAG42100.046.0691265
GCGGGGG120450.045.8881724
CGGGGAA25150.045.6370475
CGGGGTC4650.045.5114365
ATGGCGA4500.045.3606831
Produced by FastQC (version 0.11.5)