##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526731_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 284523 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.40911631045645 38.0 38.0 38.0 35.0 39.0 2 37.38796863522457 38.0 38.0 38.0 35.0 39.0 3 37.23771364705138 38.0 36.0 38.0 35.0 39.0 4 37.22819244841366 38.0 36.0 38.0 35.0 39.0 5 37.1573440459998 38.0 35.0 38.0 35.0 39.0 6 37.13638967675724 38.0 35.0 38.0 35.0 39.0 7 37.247023966428024 38.0 38.0 38.0 35.0 39.0 8 35.6004751812683 35.0 35.0 36.0 35.0 39.0 9 35.31867722468834 35.0 35.0 36.0 35.0 39.0 10 32.42064086207442 35.0 31.0 35.0 24.0 39.0 11 32.11051830607719 35.0 30.0 36.0 24.0 38.0 12 32.28822274473417 35.0 30.0 36.0 25.0 38.0 13 31.887256917718428 35.0 29.0 36.0 23.0 38.0 14 32.098617686443625 34.0 30.0 36.0 23.0 38.0 15 32.061425614097985 34.0 30.0 36.0 24.0 38.0 16 30.499913890968394 33.0 28.0 36.0 20.0 38.0 17 29.83400990429596 33.0 27.0 35.0 19.0 38.0 18 29.65283650179423 32.0 27.0 35.0 18.0 38.0 19 29.19813512440119 32.0 26.0 35.0 17.0 38.0 20 29.117438660494933 32.0 27.0 35.0 16.0 38.0 21 28.1089507702365 31.0 24.0 35.0 10.0 38.0 22 27.73956411256735 30.0 23.0 35.0 4.0 38.0 23 27.593238507958933 30.0 23.0 35.0 4.0 38.0 24 27.402572024054294 30.0 24.0 35.0 4.0 38.0 25 26.83867385062016 30.0 23.0 35.0 4.0 38.0 26 25.685269732148193 30.0 20.0 35.0 4.0 38.0 27 25.283604488916538 29.0 19.0 34.0 4.0 38.0 28 24.857972817663246 29.0 18.0 34.0 4.0 37.0 29 24.655999690710416 29.0 18.0 34.0 4.0 37.0 30 24.08563455326986 29.0 15.0 33.0 4.0 36.0 31 23.434847094962446 28.0 11.0 34.0 4.0 37.0 32 23.132372426833683 27.0 10.0 33.0 4.0 36.0 33 22.82362410068782 27.0 10.0 33.0 4.0 36.0 34 22.657567226551105 27.0 4.0 33.0 4.0 36.0 35 22.365285056041163 26.0 4.0 33.0 4.0 36.0 36 23.5452740200265 29.0 4.0 35.0 4.0 38.0 37 23.121677333642623 29.0 4.0 35.0 4.0 38.0 38 22.836846230357477 29.0 4.0 35.0 4.0 37.0 39 22.627780530923687 28.0 4.0 34.0 4.0 36.0 40 22.062873651690726 27.0 4.0 34.0 4.0 36.0 41 21.903448930314948 28.0 4.0 35.0 4.0 37.0 42 21.65550412444688 27.0 4.0 34.0 4.0 36.0 43 21.419087384851135 27.0 4.0 34.0 4.0 36.0 44 21.040819195636207 27.0 4.0 34.0 4.0 36.0 45 20.32894704470289 25.0 4.0 33.0 4.0 36.0 46 19.52006691901885 23.0 4.0 33.0 4.0 36.0 47 19.161825230297726 23.0 4.0 33.0 4.0 36.0 48 18.770503614822 22.0 4.0 33.0 4.0 36.0 49 18.370377790196223 20.0 4.0 33.0 4.0 35.0 50 17.913736323601256 17.0 4.0 33.0 4.0 35.0 51 17.07486213768307 4.0 4.0 32.0 4.0 35.0 52 16.684018515199124 4.0 4.0 32.0 4.0 35.0 53 16.314463154121107 4.0 4.0 32.0 4.0 35.0 54 15.95884691220042 4.0 4.0 32.0 4.0 35.0 55 15.355454567820528 4.0 4.0 31.0 4.0 35.0 56 9.346541404385587 4.0 4.0 17.0 4.0 22.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.46443035982935754 1 2 -0.5093124123349142 1 3 -0.6275503060816661 1 4 -0.7932524279652 1 5 -1.3502334141227905 1 6 -0.7407342856738808 1 7 -0.7574620664665943 1 8 -0.7974232505261583 1 9 -0.7917737766362904 1 10 -0.021887120201022014 1 11 -0.5988929292715532 1 12 -0.8728918130504013 1 13 0.24605583051063462 1 14 -0.31884965831237366 1 15 -0.8105552711616397 1 16 1.4429890754604173 1 17 1.4552175791451525 1 18 1.7421222354841355 1 19 1.6356045988519199 1 20 1.7670452237423433 1 21 2.8854491754780014 1 22 2.124553758565515 1 23 1.9425350242201276 1 24 2.3416580466037438 1 25 1.8691632462645025 1 26 2.453403990843743 1 27 0.932516188573878 1 28 2.3325164923168735 1 29 2.7284077349595073 1 30 2.3239295450366306 1 31 2.947157437018209 1 32 1.887777450685217 1 33 2.3178515426768804 1 34 2.207413098028905 1 35 1.5391577580611617 1 36 3.3166128258688126 1 37 3.5792055739104427 1 38 3.185803809413372 1 39 3.78775301872799 1 40 2.914214855164282 1 41 3.856553967490477 1 42 3.8771419021582894 1 43 3.75589219383793 1 44 3.70845235729157 1 45 4.063602924101048 1 46 3.0269881361005453 1 47 3.2908729410038973 1 48 2.8493727877043824 1 49 2.6264139838068274 1 50 2.2692403073788583 1 51 2.8193213509072663 1 52 2.4039344949364576 1 53 2.6979465897088737 1 54 3.008555324672466 1 55 2.5968324514713377 1 56 1.0477406895632235 2 1 -0.05814220354120181 2 2 -0.0615102145327171 2 3 -0.093362271893632 2 4 0.10601497130220139 2 5 0.12076780687842614 2 6 0.15364910870951576 2 7 0.14119483219030826 2 8 0.15923120612829678 2 9 0.18563770077518882 2 10 0.059614711300810086 2 11 -0.4767928071714316 2 12 0.11825592809734076 2 13 0.0033818378366454738 2 14 0.32583898637626874 2 15 0.4632542526478858 2 16 0.9435995760709162 2 17 0.5748756988032717 2 18 0.7610477544096526 2 19 0.9484861617334808 2 20 0.7588034655005842 2 21 -0.11149832146949734 2 22 -0.032039648027890166 2 23 -0.13469330300820204 2 24 0.44208539703109295 2 25 -0.32802845092719224 2 26 1.1206811581209095 2 27 1.3326993387570312 2 28 0.5159719257723054 2 29 0.718944975496747 2 30 1.5498147709218593 2 31 1.150148890009664 2 32 1.244920307828071 2 33 1.0980713228966614 2 34 1.4110150516308586 2 35 1.516569235472641 2 36 1.3224125816685692 2 37 1.6924534371583064 2 38 1.848807472417036 2 39 1.598497829472798 2 40 1.6187325596819875 2 41 2.141657752594263 2 42 1.9314764564928453 2 43 1.86730855525429 2 44 2.2130311118703254 2 45 2.6167164772146023 2 46 2.1838867929992034 2 47 2.221275871406828 2 48 1.8844765728081683 2 49 2.128245485638331 2 50 2.2319997701383194 2 51 2.37304540463132 2 52 1.9430065340085 2 53 1.9473360890983713 2 54 2.0641108802280232 2 55 1.5495186541575396 2 56 0.47753311935565357 3 1 -0.06363670903571261 3 2 -0.17716955519205868 3 3 -0.09024871878008156 3 4 -0.09563338034615043 3 5 -0.2010270649164454 3 6 -0.12922634916593978 3 7 -0.12611286011738798 3 8 0.11527516217225298 3 9 0.11933733447482098 3 10 -0.5564109297248301 3 11 -0.6818294372080622 3 12 -0.11297117812976865 3 13 0.2960558305106389 3 14 -0.4283734678361846 3 15 -0.06769812830449595 3 16 -0.21693155946021747 3 17 -0.36303638910881375 3 18 -0.4047031613412635 3 19 -0.4076493694020513 3 20 -0.20795477625765812 3 21 -0.1189159038870784 3 22 -0.3183033842916281 3 23 0.12070962739473146 3 24 0.2091183640640608 3 25 -0.15385262675136957 3 26 0.5006262130659636 3 27 -0.01391238285469143 3 28 0.8448180796184594 3 29 0.8637251952769667 3 30 0.5140089101159973 3 31 0.22057013543090775 3 32 0.7734917363994995 3 33 0.8630896379149746 3 34 0.7232861139019207 3 35 0.7157450596484658 3 36 1.0241525084084948 3 37 1.0919039866088553 3 38 1.2774704760800404 3 39 0.34053079650576734 3 40 0.6717545377039649 3 41 1.1402841262206351 3 42 0.7132530132694015 3 43 0.19120965415539004 3 44 0.507261881101094 3 45 0.33503149552961986 3 46 0.6916706757830866 3 47 0.9408729410038994 3 48 1.229531517863112 3 49 0.9371282695211143 3 50 1.8113037994423493 3 51 0.6558292874152052 3 52 0.9690138600158242 3 53 0.5531053198676013 3 54 0.6760156421327839 3 55 0.7496102292491127 3 56 -0.3423386755161424 4 1 -0.07310931528250819 4 2 -0.1369365802816631 4 3 -0.20793945098694167 4 4 -0.41409216170815455 4 5 -0.3695626511939665 4 6 -0.09685307727654191 4 7 -0.1327820869479055 4 8 -0.12589227254356672 4 9 -0.26258790874074833 4 10 -0.6607760090899077 4 11 -0.708979974842471 4 12 -0.2665170050626884 4 13 -0.778649750646558 4 14 -0.3541799194490878 4 15 -0.4619889629179106 4 16 -0.5887569562856143 4 17 -0.010220208873278125 4 18 -0.36463659708114804 4 19 -0.49099550355463606 4 20 -0.09022564159304025 4 21 -0.11890310306782581 4 22 -0.2665112695962861 4 23 0.10548945330358706 4 24 -0.07526463644797232 4 25 -0.2478362417027249 4 26 0.06400306918475707 4 27 -0.09193337619826636 4 28 0.4245543827418601 4 29 0.4963288819129126 4 30 0.21845079439659187 4 31 0.954197887607048 4 32 0.5833739688623787 4 33 -0.0769257230681255 4 34 1.0902856018691516 4 35 1.2501152593412463 4 36 1.2378877874663559 4 37 1.0642286153850975 4 38 1.1704428263104525 4 39 1.1077735000387925 4 40 1.165622945282049 4 41 0.5168202245309281 4 42 0.7253241858244444 4 43 0.39785327934740167 4 44 1.0933729922122062 4 45 0.6027734310134925 4 46 0.7891489143903563 4 47 0.6974269604611436 4 48 0.26168717582522305 4 49 0.7267724067458943 4 50 0.5557226422482877 4 51 0.4217279049267262 4 52 0.4118710028729673 4 53 1.0541933895040607 4 54 0.38413136153882554 4 55 0.9254294816812685 4 56 0.1880376685698799 5 1 0.12846285806385538 5 2 0.1811637781412756 5 3 -0.004534433065792598 5 4 0.21648783177506203 5 5 0.13479544590606451 5 6 0.2174186724790772 5 7 0.22702566802114177 5 8 0.3100803569774442 5 9 0.26360140373888896 5 10 0.4061431828292825 5 11 0.7151402597616325 5 12 -0.13353394869253776 5 13 0.6616402460950539 5 14 0.32238410792139405 5 15 0.8750507894444226 5 16 0.7912935487648909 5 17 1.1217038706314462 5 18 -0.21963822627632723 5 19 1.3534978167451364 5 20 1.3092530159501337 5 21 1.5934217584505816 5 22 1.4892723732841304 5 23 1.6966836533687548 5 24 1.1087936887393859 5 25 1.4236365507378075 5 26 2.7313621438018956 5 27 1.7387932448509353 5 28 2.0946016294020104 5 29 2.685478442030213 5 30 2.209571680678767 5 31 3.038427278288051 5 32 2.233015545923312 5 33 2.744799594624933 5 34 2.8304289710447783 5 35 2.304489648393055 5 36 2.096555105811092 5 37 1.9383325580374269 5 38 2.024548398157961 5 39 2.056331662306633 5 40 2.2044385203879457 5 41 2.3632278491643604 5 42 2.17872920374559 5 43 2.1285473164930515 5 44 2.7284740023132166 5 45 2.377455737953863 5 46 2.0103936195060292 5 47 1.9721499972809546 5 48 1.9285574918890873 5 49 1.9748988322916752 5 50 1.6146587777973274 5 51 2.068599850185766 5 52 1.0899013059032718 5 53 1.1624126791749632 5 54 1.0852147763319184 5 55 1.0331600127988967 5 56 0.3000855669081002 6 1 0.0939263161743753 6 2 0.18238926833735292 6 3 0.23443615516950445 6 4 0.23598426671267703 6 5 0.21010738886506886 6 6 0.3496602054559048 6 7 0.28974848442042855 6 8 0.29437180082771164 6 9 0.4044213680882649 6 10 -0.037818492750041344 6 11 0.9372882098507631 6 12 0.042491006744178605 6 13 0.5984717968971935 6 14 0.47089123804617117 6 15 0.7225329641324763 6 16 0.6767659195313804 6 17 0.9071316781180769 6 18 1.163048939499074 6 19 0.6290102944634945 6 20 0.9529695934902414 6 21 1.4179538440120787 6 22 1.8897848510024886 6 23 1.8671381988233016 6 24 1.6144755069212025 6 25 1.7293406505595534 6 26 2.7081892382760486 6 27 1.551738877649509 6 28 2.5579483317193024 6 29 2.9077378003189835 6 30 2.401193784065576 6 31 2.692839042993935 6 32 2.5222312321978215 6 33 3.443551822788926 6 34 2.1282230886918363 6 35 2.5088122865392215 6 36 2.936127298324461 6 37 3.4640678521550754 6 38 2.557302408853147 6 39 3.478345922556187 6 40 2.6104099998888373 6 41 3.6749479917668566 6 42 3.6689252821769642 6 43 3.7655794020545486 6 44 4.702850116395211 6 45 3.811502083764914 6 46 3.833477398472162 6 47 4.266713277138354 6 48 4.346338240552189 6 49 4.0639139838068274 6 50 3.1956525389381483 6 51 2.5105561781715053 6 52 3.4192239440494383 6 53 2.8929162442373517 6 54 3.7367299278470707 6 55 3.8430976242070987 6 56 1.4363377950720935 7 1 -0.11740877966171581 7 2 -0.05385494845048555 7 3 -0.05228724205455393 7 4 -0.009678324166372931 7 5 0.026394015875425225 7 6 0.20192667384101526 7 7 0.16156771773172807 7 8 0.2624126688603212 7 9 0.48172363731055867 7 10 0.2842926550798808 7 11 0.6347435964998027 7 12 0.5165767084405886 7 13 0.2550178422816387 7 14 0.2199411389053907 7 15 0.44131738801439013 7 16 1.159058274675683 7 17 0.9166051304203435 7 18 1.600246009338246 7 19 1.23543516778361 7 20 1.0317589744111473 7 21 1.5853778360808164 7 22 1.6324456793787832 7 23 0.9289359516322904 7 24 1.2448594020522883 7 25 1.2286246338157802 7 26 2.374663131204006 7 27 1.8176151719874696 7 28 1.9897752759801506 7 29 1.8392067362079487 7 30 3.0196670160550028 7 31 2.691985330722506 7 32 2.445562362402711 7 33 1.9663266630621123 7 34 2.1132005066253043 7 35 2.514099794800952 7 36 2.5980021606289334 7 37 2.8807482883745053 7 38 2.7224544247156715 7 39 2.7667078965592715 7 40 2.515427624916377 7 41 2.5848400384731782 7 42 2.4240475558055152 7 43 2.4627318585427638 7 44 3.000988472861394 7 45 2.1031962895371095 7 46 3.0832570856279204 7 47 3.3566835349043807 7 48 2.8610992011161898 7 49 2.8923315868030812 7 50 2.9985964693192884 7 51 3.677980170240243 7 52 3.2620582687905717 7 53 3.4723803332437413 7 54 3.4263366694201043 7 55 3.748473257606525 7 56 1.8062568301018356 8 1 -0.4168733161785667 8 2 -0.3787320551920601 8 3 -0.2193781830657926 8 4 -0.397977130346149 8 5 -0.23037974348787316 8 6 -0.40355670630879814 8 7 -0.30446107440310044 8 8 -0.032604302113462325 8 9 -0.19851980838232208 8 10 -0.05964753686768631 8 11 0.9947330627919371 8 12 0.7927207861559467 8 13 0.4240692233677805 8 14 0.5903765321638161 8 15 0.6308509788383603 8 16 1.2215729048254964 8 17 2.1934368251768994 8 18 2.2096941600873095 8 19 1.8342033091693786 8 20 2.2503041523137703 8 21 2.6509501675414917 8 22 2.9152903657083726 8 23 2.2772944488233016 8 24 3.1613505069212025 8 25 3.512888444677202 8 26 3.4747333559231066 8 27 2.867226010002451 8 28 3.5202645081898893 8 29 3.1094841238483966 8 30 3.4356606958302827 8 31 3.444677278288051 8 32 3.652286379256644 8 33 3.6508128522006906 8 34 4.744491471044778 8 35 4.5678655953627505 8 36 3.8177909012656386 8 37 4.008645058037427 8 38 4.083497261794324 8 39 4.0024727607914805 8 40 4.52700007341825 8 41 4.782025197649208 8 42 5.196958370412258 8 43 4.455606975583962 8 44 5.4431993811010955 8 45 5.185812745529621 8 46 4.842898354354514 8 47 4.09667651243247 8 48 5.359665446434541 8 49 4.6732889838068274 8 50 4.696571656585206 8 51 5.208396251700918 8 52 5.263433502872967 8 53 5.315940141296174 8 54 5.142979927847071 8 55 5.360101300677686 8 56 3.295942574483858 9 1 0.03048397172756978 9 2 0.06714452423032213 9 3 0.03066412289088305 9 4 0.06483593373327068 9 5 0.17780222853378547 9 6 0.22852249044210282 9 7 0.19308518010953435 9 8 0.26380533236307 9 9 0.2901986031700261 9 10 0.4278722059120952 9 11 1.4707878913117938 9 12 0.8851593014989092 9 13 0.857324525714354 9 14 1.062962272236021 9 15 1.2463039346145308 9 16 1.4682025302767592 9 17 1.0821157511696775 9 18 1.990732630303917 9 19 1.6581964274004264 9 20 2.0430895765014974 9 21 2.238523497866403 9 22 2.3075088900766048 9 23 2.1866327836608477 9 24 2.5483653986179533 9 25 2.6596337064100553 9 26 2.3834653053815913 9 27 2.3712732798580483 9 28 2.6362956453740054 9 29 2.988235252007243 9 30 3.5090192247111496 9 31 2.957199841464945 9 32 3.5022694568739716 9 33 2.578638890106827 9 34 2.806963267073659 9 35 2.871946709983689 9 36 3.117641420218707 9 37 3.206834363091577 9 38 3.3295205018665257 9 39 2.8429934060983406 9 40 3.5611691844290796 9 41 2.779853491873034 9 42 3.027706339726336 9 43 3.750634615475658 9 44 3.386594011064993 9 45 3.035753517190269 9 46 3.7660002270981963 9 47 3.5879332813855385 9 48 4.348355654015769 9 49 3.6950060415685613 9 50 3.5408293280653496 9 51 3.4899705973687887 9 52 3.2782969956527523 9 53 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120 38 1.507749979185629 120 39 1.1131498441248127 120 40 1.5165267944327425 120 41 1.586712697649208 120 42 1.6606132617166054 120 43 1.6499570661636724 120 44 1.4279140550141385 120 45 1.9719880172687496 120 46 2.016981234789295 120 47 1.8007073095339194 120 48 2.2073783087533805 120 49 2.0249647084445073 120 50 1.6909104247011477 120 51 1.9230135524255552 120 52 1.3031753506990533 120 53 2.1372150507164633 120 54 1.7204255800209847 120 55 2.018502568793627 120 56 1.1717917592664673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 201.0 5 579.0 6 503.0 7 263.0 8 1619.0 9 1358.0 10 1426.0 11 4746.0 12 5354.0 13 6277.0 14 8179.0 15 7069.0 16 8376.0 17 8323.0 18 8379.0 19 7658.0 20 7132.0 21 7384.0 22 8396.0 23 9160.0 24 9859.0 25 10569.0 26 11812.0 27 13310.0 28 14827.0 29 15952.0 30 17154.0 31 17544.0 32 17174.0 33 16349.0 34 13845.0 35 9934.0 36 6775.0 37 4415.0 38 2043.0 39 579.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 98.46339311760384 1.237861262534136 0.20982486477367385 0.08892075508834084 8 98.5313244795658 1.139295682557984 0.18138740271518664 0.14799243516103405 9 94.82642879486018 3.763843344826253 0.3746621538504796 1.035065706463098 10 42.56000393641287 40.848718732756225 7.162162637115453 9.429114693715448 11 39.24568488312017 21.99540986141718 21.126587305771412 17.632317949691238 12 33.202939656899446 12.90475638173364 26.86707225777881 27.02523170358811 13 24.617342007500273 19.24800455499204 29.012768739258338 27.12188469824935 14 24.757928181552984 22.26568678103352 27.78685730151868 25.189527735894814 15 30.373642904088598 22.51241551649603 25.123100768654904 21.990840810760464 16 35.67901364740285 23.124668304495593 22.819596306801206 18.376721741300354 17 30.775367896444223 23.08214098684465 24.56778538114669 21.574705735564436 18 30.550781483395017 23.35628402624744 24.0216080949519 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25.696886827693632 22.041006195332706 32 33.6604600682209 19.080004510472754 26.010980182110337 21.24855523919601 33 33.538695783960584 19.32709726990124 26.764202082290662 20.370004863847516 34 27.94253987838195 21.768573191151848 31.71622455274522 18.572662377720984 35 25.326878050156566 26.531425428079775 29.346032328133376 18.795664193630284 36 26.105298802813426 27.92012642064494 26.272848485703605 19.70172629083803 37 28.407900353110083 26.247967235899406 25.188636980961686 20.155495430028818 38 27.60986248001778 26.17643633745858 25.647459038665804 20.56624214385783 39 29.925687960037422 23.024376467265633 25.438723456833706 21.61121211586324 40 26.51900522820404 21.73979087183835 26.079906740144125 25.66129715981348 41 24.311041704942127 24.17289673398437 26.452465410760468 25.063596150313032 42 22.64829048502518 21.712518773743263 26.593868716317697 29.04532202491386 43 23.742461840830842 20.524648808387592 28.090380142385484 27.642509208396078 44 25.117809885071285 19.988970237316657 26.99746527569104 27.89575460192102 45 26.979760406206243 21.548944174928927 26.372643310750604 25.098652108114223 46 25.144502536311347 24.135991566853203 26.20465945509986 24.51484644173559 47 23.98854975337386 21.61570198220895 28.300791892829086 26.0949563715881 48 25.968761726742994 20.023081770357624 29.668571266736055 24.339585236163327 49 26.93682660588127 20.356500686667328 27.571109003128935 25.13556370432247 50 24.45428919485523 20.24123179783557 28.593333899937672 26.71114510737152 51 23.64630908125332 20.866702071163036 29.77305717826164 25.713931669322005 52 23.462482338067968 20.482883418854268 29.55730964952353 26.497324593554232 53 23.518809027199875 20.430891919360036 31.351565736868388 24.6987333165717 54 23.890175039327666 19.931368317417963 30.716958517783954 25.461498125470417 55 24.076508036603123 20.5068887700118 27.546577579701776 27.870025613683303 56 24.47328525482084 22.531843749557233 24.82678983833718 28.168081157284746 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 9.5 11 18.0 12 48.0 13 143.0 14 208.0 15 226.0 16 244.0 17 343.0 18 442.0 19 707.5 20 973.0 21 1119.0 22 1221.5 23 1178.0 24 1446.5 25 1715.0 26 2148.5 27 2582.0 28 2814.5 29 3439.0 30 3831.0 31 4183.0 32 4535.0 33 4619.5 34 4704.0 35 4696.5 36 4689.0 37 4860.5 38 5308.0 39 5584.0 40 6155.0 41 6726.0 42 7138.0 43 7550.0 44 7912.0 45 8274.0 46 8656.5 47 9941.0 48 10843.0 49 11097.5 50 11352.0 51 11720.5 52 12089.0 53 12289.0 54 13003.5 55 13518.0 56 14063.5 57 14609.0 58 15195.0 59 15781.0 60 17033.0 61 18285.0 62 19011.5 63 20096.0 64 20454.0 65 18507.5 66 16561.0 67 14430.5 68 12300.0 69 11018.5 70 9737.0 71 8228.0 72 5908.0 73 5097.0 74 4615.5 75 4134.0 76 3522.0 77 2910.0 78 2546.0 79 1785.5 80 1389.0 81 1190.0 82 991.0 83 814.5 84 638.0 85 489.0 86 340.0 87 278.0 88 165.0 89 114.0 90 97.0 91 80.0 92 69.5 93 59.0 94 62.0 95 65.0 96 91.0 97 58.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.01722180632145732 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.001054396305395346 21 0.0 22 0.0 23 0.0 24 0.0045690506567131655 25 0.013707151970139496 26 0.0604520548426665 27 0.05939765853727115 28 0.0857575661721548 29 0.1398832431824492 30 0.21439391543038702 31 0.2674651961352861 32 0.2597329565623869 33 0.28011795180003024 34 0.2980426889917511 35 0.327214320107689 36 0.3602520710100765 37 0.36622698340731685 38 0.4027793886610221 39 0.4421435173957817 40 0.5071646228951614 41 0.5226291020409598 42 0.5440684935839984 43 0.5725371938296728 44 0.5806208988377038 45 0.601357359510479 46 0.6428302808560292 47 0.6709475156665718 48 0.7201526765850212 49 0.7025794048284321 50 0.7517845657468816 51 0.728587847028184 52 0.7507301694414863 53 0.7493243077009591 54 1.4712343114616393 55 0.791500159916773 56 0.7756842153358429 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 284523.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.452841687652324 #Duplication Level Percentage of deduplicated Percentage of total 1 76.86511496363777 41.85523936418163 2 10.64969880663285 11.598127262775169 3 4.566619377093101 7.459962059658482 4 2.4484242316345393 5.332946282776294 5 1.4104558110194567 3.8401663492435865 6 0.9611038306768327 3.1400900842345063 7 0.6545057126303531 2.4947787167467275 8 0.4458499612253479 1.9422237884039837 9 0.31621830630320874 1.549708683467956 >10 1.5770892421332487 14.860031009655126 >50 0.07932957237584388 2.7785557959959943 >100 0.024310675405500547 2.2709886201226035 >500 6.397546159342249E-4 0.26719562381255363 >1k 6.397546159342249E-4 0.6099863589253994 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1751 0.6154159769157502 No Hit ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCC 767 0.26957398874607674 No Hit ATGGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 415 0.1458581555796895 No Hit ATGGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 396 0.13918031231218564 No Hit ATGGCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 303 0.10649402684492994 No Hit ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 290 0.10192497618821676 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0024602580459224736 0.0 0.0 0.0 0.0 11 0.0038661197864496015 0.0 0.0 0.0 0.0 12 0.00527198152697673 0.0 0.0 0.0 0.0 13 0.006326377832372075 0.0 0.0 0.0 0.0 14 0.007380774137767421 0.0 0.0 0.0 0.0 15 0.008083705008030986 0.0 0.0 0.0 0.0 16 0.009138101313426331 0.0 0.0 0.0 0.0 17 0.010192497618821676 0.0 0.0 0.0 0.0 18 0.011598359359348805 0.0 0.0 0.0 0.0 19 0.01441008284040306 0.0 0.0 0.0 0.0 20 0.017221806321457315 0.0 0.0 0.0 0.0 21 0.017924737191720882 0.0 0.0 0.0 0.0 22 0.020384995237643352 0.0 0.0 0.0 0.0 23 0.02108792610790692 0.0 0.0 0.0 0.0 24 0.0214393915430387 0.0 0.0 0.0 0.0 25 0.022142322413302263 0.0 0.0 0.0 0.0 26 0.02319671871869761 0.0 0.0 0.0 0.0 27 0.02354818415382939 0.0 0.0 0.0 0.0 28 0.02354818415382939 0.0 0.0 0.0 0.0 29 0.0253055113294883 0.0 0.0 0.0 0.0 30 0.026359907634883648 0.0 0.0 0.0 0.0 31 0.027765769375410775 0.0 0.0 0.0 0.0 32 0.029523096551069685 0.0 0.0 0.0 0.0 33 0.03022602742133325 0.0 0.0 0.0 0.0 34 0.031280423726728596 0.0 0.0 0.0 0.0 35 0.03338921633751929 0.0 0.0 0.0 0.0 36 0.03444361264291463 0.0 0.0 0.0 0.0 37 0.03795826699423245 0.0 0.0 0.0 0.0 38 0.040067059605023145 0.0 0.0 0.0 0.0 39 0.04252731765094562 0.0 0.0 0.0 0.0 40 0.044284644826604526 0.0 0.0 0.0 0.0 41 0.046744902872527 0.0 0.0 0.0 0.0 42 0.04779929917792235 0.0 0.0 0.0 0.0 43 0.049205160918449474 0.0 0.0 0.0 0.0 44 0.05025955722384482 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTACA 15 0.008251471 50.26313 47 GCGTAGA 25 2.049612E-5 50.138584 38 GTGAATC 20 4.1688417E-4 50.023487 29 ACCTCGT 30 1.0429485E-6 50.014656 26 GATACGG 15 0.008550613 49.81238 20 CGGGGAT 3260 0.0 49.33663 5 TGGCGGG 28170 0.0 48.751953 2 ATGGCGG 28585 0.0 48.69756 1 GCGGGGA 11640 0.0 48.61844 4 GGCGGGG 27340 0.0 47.827843 3 CGGGGAC 2020 0.0 47.69954 5 GGCGGGA 1065 0.0 47.45708 3 GCGGGGT 1610 0.0 47.165943 4 CGGGGGA 2815 0.0 47.1415 5 CGGGATA 70 0.0 46.23809 5 CGGGGAG 4210 0.0 46.069126 5 GCGGGGG 12045 0.0 45.888172 4 CGGGGAA 2515 0.0 45.637047 5 CGGGGTC 465 0.0 45.511436 5 ATGGCGA 450 0.0 45.360683 1 >>END_MODULE