FastQCFastQC Report
Fri 17 Jun 2016
SRR1526730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57004
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2610.4578626061329029No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.37365798891305874No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.28419058311697426No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.271910743105747No Hit
CCGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.15963792014595468No Hit
CCGACGGGGCTATCACCACAGCTTTTTCTCCATAAGGCTTCTCACACCTCACACCCTCCTCCTG630.11051856010104555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAAAC150.004499281358.52972456
ACGGGCA258.4182975E-658.1684344
ACGGGAT258.4182975E-658.1684344
ACGGGAA258.4182975E-658.1684344
ACGGGGT3800.058.168434
CGGGATG201.9656641E-458.1684275
GACGGGT201.9656641E-458.1684273
TCTGAAA150.00464341258.0660255
ATCTATC150.00464341258.0660250
CTATCTG150.00464341258.0660252
ATTAAGC258.506868E-658.0660251
TATCTGA150.00464341258.0660253
TCTATCT150.00464341258.0660251
CATGCCC201.9828901E-458.06601753
GGTACCT453.092282E-1158.014958
TCCTCCA201.991548E-458.0149539
GGTCAGT201.991548E-458.014958
GGAACGG150.00465963658.0149548
GATCTAT150.00465963658.0149549
GGATTTC150.00465963658.014958
Produced by FastQC (version 0.11.5)