FastQCFastQC Report
Fri 17 Jun 2016
SRR1526729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82026
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6570.8009655475093262No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.4644868700168239No Hit
CCGACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3550.4327896032965157No Hit
CCGACGGGGCTATCACCACAGCTTTTTCTCCATAAGGCTTCTCACACCTCACACCCTCCTCCTG3010.3669568185697218No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2670.3255065467047034No Hit
CCGACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2450.2986857825567503No Hit
CCGACGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC1840.22431911832833493No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1800.21944261575597984No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.21700436446980223No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1770.21578523882671347No Hit
CCGACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1580.19262185160802672No Hit
CCGACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1540.1877453490356716No Hit
CCGACGGGGGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTCTGACATGCCCAGATA1530.18652622339258285No Hit
CCGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1520.18530709774949405No Hit
CCGACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1470.17921146953405018No Hit
CCGACGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1420.1731158413186063No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1420.1731158413186063No Hit
CCGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1140.13898032331212054No Hit
CCGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG1130.13776119766903178No Hit
CCGACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1110.13532294638285422No Hit
CCGACGGGGGGTGGCATCTGGTATCTGGATGCTCCACCCTAAAGTGGGAGACCTCATCCTTGCT1080.13166556945358787No Hit
CCGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC1080.13166556945358787No Hit
CCGACGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTG1070.1304464438104991No Hit
CCGACGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC1050.12800819252432155No Hit
CCGACGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA1040.1267890668812328No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTC1020.12435081559505523No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC970.11825518737961134No Hit
CCGACGGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCC970.11825518737961134No Hit
CCGACGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG940.11459781045034502No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT930.11337868480725624No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTC920.11215955916416746No Hit
CCGACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC900.10972130787798992No Hit
CCGACGGGGCTGCGGGGAGGTGGGAGAGCCGGGAAAGTCTGGTATCCCCTGCCCCAGGGTCCTA890.10850218223490112No Hit
CCGACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG880.10728305659181234No Hit
CCGACGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC860.1048448053056348No Hit
CCGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC850.10362567966254602No Hit
CCGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGC830.10118742837636847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCAAC150.004633572458.120153
GATGTGA150.004633572458.120155
ATGTGAT201.9789363E-458.120156
GTGATGA150.004633572458.120158
CCTGTGC150.004633572458.120154
CCTGTCA150.004633572458.120157
CCAGATA258.493182E-658.120158
CCCAACC150.004633572458.120154
GTTGTCC150.004633572458.120152
AGGTTGT150.004633572458.120150
AGACAAG150.004633572458.120154
AACCTAC150.004633572458.120157
GACGCGT150.004633572458.120158
ATGCCCA258.493182E-658.120154
CGCCCAA150.004633572458.120152
TCAAAAG150.00464483358.08450737
TGCCCGT150.00464483358.08450738
CGCATCT150.00464483358.08450741
ACATTGA150.00464483358.08450744
CGACATT150.00464483358.08450742
Produced by FastQC (version 0.11.5)