FastQCFastQC Report
Fri 17 Jun 2016
SRR1526728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82241
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6420.7806325312192216No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.40977128196398394No Hit
CCGACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3020.36721343368879267No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2790.33924684767938135No Hit
CCGACGGGGCTATCACCACAGCTTTTTCTCCATAAGGCTTCTCACACCTCACACCCTCCTCCTG2640.3210077698471565No Hit
CCGACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2010.24440364295181236No Hit
CCGACGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC1810.22008487250884592No Hit
CCGACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1670.2030617331987695No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1570.19090234797728625No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1560.18968640945513796No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.18847047093298963No Hit
CCGACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1550.18847047093298963No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1520.18482265536654469No Hit
CCGACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1490.1811748398000997No Hit
CCGACGGGGGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTCTGACATGCCCAGATA1480.1799589012779514No Hit
CCGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1480.1799589012779514No Hit
CCGACGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1470.17874296275580306No Hit
CCGACGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA1230.1495604382242434No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1110.13496917595846353No Hit
CCGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC1110.13496917595846353No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTC1060.12888948334772193No Hit
CCGACGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC1050.1276735448255736No Hit
CCGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG990.12037791369268369No Hit
CCGACGGGGGGTGGCATCTGGTATCTGGATGCTCCACCCTAAAGTGGGAGACCTCATCCTTGCT970.11794603664838706No Hit
CCGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG860.10457071290475553No Hit
CCGACGGGGGGTGGTCGTCCGGTATCCGAAGTCCCCGTGTTCGTCATGTCGGCGTCTGTGGTGT860.10457071290475553No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTC850.10335477438260722No Hit
CCGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.10092289733831057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATAT201.9229097E-458.45845826
AAACCGT150.004528230558.45845826
GACAGCT150.00453928358.4225145
CGAGTTT150.00453928358.4225143
GCGAAAA150.00453928358.4225145
CCCAACA150.00453928358.4225143
TGCGAAA150.00453928358.4225144
CACACCT150.00453928358.4225143
GTGCGAA150.00453928358.4225143
CGGACTG150.00453928358.4225143
AGCACAT201.9346512E-458.386624
AAAAGGT150.004550355558.386625
AAAACCG150.004550355558.386625
GCACATA201.9346512E-458.386625
TATCTGG201.9346512E-458.386622
CGTGTTT150.004550355558.386640
ATGCGGC201.9346512E-458.386624
CGATGCG201.9346512E-458.386622
ACGTAGA150.004550355558.386641
AACTAAG150.004550355558.386642
Produced by FastQC (version 0.11.5)