SRR1526727_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526727_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76153
Sequences flagged as poor quality	0
Sequence length	56
%GC	58

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	698	0.9165758407416649	No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	367	0.4819245466363768	No Hit
CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	289	0.3794991661523512	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	272	0.35717568579044817	No Hit
CCGACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	253	0.33222591362126247	No Hit
CCGACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG	248	0.3256601841030557	No Hit
CCGACGGGGCTATCACCACAGCTTTTTCTCCATAAGGCTTCTCACACCTCACACCC	232	0.304649849644794	No Hit
CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	165	0.21666907410082334	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	164	0.21535592819718197	No Hit
CCGACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT	157	0.2061639068716925	No Hit
CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	148	0.19434559373892032	No Hit
CCGACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	139	0.18252728060614815	No Hit
CCGACGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	128	0.16808267566609325	No Hit
CCGACGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	125	0.1641432379551692	No Hit
CCGACGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	121	0.1588906543406038	No Hit
CCGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	116	0.152324924822397	No Hit
CCGACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	114	0.1496986330151143	No Hit
CCGACGGGGGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTCTGACATG	107	0.14050661168962483	No Hit
CCGACGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG	102	0.13394088217141806	No Hit
CCGACGGGGGGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCGCCTAGCTCT	97	0.12737515265321128	No Hit
CCGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG	94	0.12343571494228725	No Hit
CCGACGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	93	0.12212256903864588	No Hit
CCGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGT	92	0.12080942313500453	No Hit
CCGACGGGGGGTGGCATCTGGTATCTGGATGCTCCACCCTAAAGTGGGAGACCTCA	91	0.11949627723136318	No Hit
CCGACGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	89	0.11686998542408046	No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCCAGTGGTTAAGAGCACTGACTGCTCTTC	86	0.1129305477131564	No Hit
CCGACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	86	0.1129305477131564	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC	85	0.11161740180951506	No Hit
CCGACGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA	84	0.1103042559058737	No Hit
CCGACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	83	0.10899111000223236	No Hit
CCGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	82	0.10767796409859098	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	82	0.10767796409859098	No Hit
CCGACGGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG	81	0.10636481819494964	No Hit
CCGACGGGGAAGACCTTGCCCCTCCAGACCTGGGCCGAGACGACAAGGGGAGCCGC	81	0.10636481819494964	No Hit
CCGACGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	80	0.1050516722913083	No Hit
CCGACGGGGGAGAGAAGGCCACTGGCCGCTATTATGCCATGAAGATCCTGCGCAAG	79	0.10373852638766692	No Hit
CCGACGGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCT	77	0.10111223458038424	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTAT	20	3.925785E-4	50.58761	50
CTAGTTA	20	3.925785E-4	50.58761	49
AATATCA	15	0.00802141	50.587605	49
ATATCAG	15	0.00802141	50.587605	50
ACAGAGA	15	0.00802141	50.587605	50
GCTCTAG	20	4.09666E-4	50.15324	46
ACACCTC	25	2.0317635E-5	50.153233	44
CTATATA	15	0.008299813	50.153233	46
GCGCCTA	15	0.008299813	50.153233	45
ACCTCAC	25	2.0317635E-5	50.153233	46
TAGCAAG	15	0.008299813	50.153233	47
ACAAGCC	15	0.008299813	50.153233	45
TACAAGC	15	0.008299813	50.153233	44
CCTACAA	15	0.008321522	50.120132	42
ATACGCT	20	4.1100453E-4	50.120132	42
AGTATAC	20	4.123465E-4	50.087074	39
TATGGTG	15	0.008343273	50.08707	41
TTCGTCA	25	2.0477168E-5	50.08707	40
CACCTAC	15	0.008343273	50.08707	40
GTTCGTC	30	1.022514E-6	50.08707	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content