##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526727_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 76153 Sequences flagged as poor quality 0 Sequence length 56 %GC 58 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.7946633750476 39.0 39.0 40.0 38.0 40.0 2 38.70551389964939 39.0 39.0 40.0 37.0 40.0 3 38.25388362901002 39.0 38.0 40.0 35.0 40.0 4 38.14489251900779 39.0 38.0 40.0 35.0 40.0 5 38.55490919596076 39.0 39.0 40.0 36.0 40.0 6 38.325778367234385 39.0 38.0 40.0 35.0 40.0 7 38.790763331713784 39.0 39.0 40.0 38.0 40.0 8 38.7547043451998 39.0 39.0 40.0 38.0 40.0 9 38.68405709558389 39.0 38.0 40.0 37.0 40.0 10 37.89649783987499 39.0 38.0 40.0 35.0 40.0 11 37.57958320749018 39.0 37.0 40.0 33.0 40.0 12 37.539821149527924 39.0 37.0 40.0 33.0 40.0 13 37.494675193360735 39.0 37.0 40.0 33.0 40.0 14 37.7729176788833 39.0 38.0 40.0 34.0 40.0 15 37.721232256115975 39.0 38.0 40.0 33.0 40.0 16 37.53141701574462 39.0 37.0 40.0 33.0 40.0 17 37.437120008404136 39.0 37.0 40.0 33.0 40.0 18 37.2883143146035 39.0 37.0 40.0 33.0 40.0 19 37.18808188777855 39.0 36.0 40.0 33.0 40.0 20 37.152797657347705 39.0 36.0 40.0 33.0 40.0 21 36.944952923719356 39.0 36.0 40.0 33.0 40.0 22 36.78058645096057 39.0 36.0 40.0 32.0 40.0 23 36.58670045828792 38.0 36.0 40.0 31.0 40.0 24 36.512179428256275 38.0 36.0 40.0 31.0 40.0 25 36.33545625254422 38.0 35.0 40.0 31.0 40.0 26 35.82566674983257 38.0 35.0 39.0 30.0 40.0 27 35.604152167347316 38.0 35.0 39.0 29.0 40.0 28 35.34269168647329 38.0 35.0 39.0 29.0 40.0 29 35.18185757619529 38.0 34.0 39.0 29.0 40.0 30 34.86345908893937 38.0 34.0 39.0 28.0 40.0 31 34.10855777185403 38.0 33.0 39.0 25.0 40.0 32 33.88850078132181 37.0 33.0 39.0 24.0 40.0 33 33.73465260725119 37.0 33.0 39.0 24.0 40.0 34 33.7091250508844 37.0 33.0 39.0 24.0 40.0 35 33.449371659685106 36.0 33.0 39.0 23.0 40.0 36 33.378133494412566 37.0 33.0 39.0 22.0 40.0 37 33.166099825351594 36.0 33.0 39.0 21.0 40.0 38 32.88225020682048 36.0 32.0 39.0 19.0 40.0 39 32.741769858048926 36.0 32.0 39.0 17.0 40.0 40 32.35377463790002 36.0 31.0 39.0 14.0 40.0 41 31.80993526190695 36.0 31.0 39.0 4.0 40.0 42 31.534686749044685 36.0 31.0 39.0 4.0 40.0 43 31.326237968300656 35.0 30.0 39.0 4.0 40.0 44 30.99739997111079 35.0 30.0 39.0 4.0 40.0 45 30.60589865139916 35.0 30.0 39.0 4.0 40.0 46 29.80909484852862 35.0 29.0 38.0 4.0 40.0 47 29.55170511995588 35.0 28.0 38.0 4.0 40.0 48 29.216472102215278 34.0 28.0 38.0 4.0 40.0 49 28.83896891783646 34.0 27.0 38.0 4.0 40.0 50 28.387863905558547 34.0 26.0 38.0 4.0 40.0 51 27.360077738237496 33.0 20.0 38.0 4.0 39.0 52 26.98462306146836 33.0 17.0 37.0 4.0 39.0 53 26.617690701613856 33.0 10.0 37.0 4.0 39.0 54 26.11885283573858 33.0 4.0 37.0 4.0 39.0 55 25.75696295615406 33.0 4.0 37.0 4.0 39.0 56 15.186138431841162 18.0 4.0 23.0 4.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5665643699130314 1 2 -0.9925805592978492 1 3 -0.8648296536204896 1 4 -0.4753363505853514 1 5 -1.0527998317596996 1 6 -0.7861215092453477 1 7 -0.7730272571730339 1 8 -1.0051826655401186 1 9 -0.3846197133059803 1 10 -0.541877272525042 1 11 -1.2767024857459006 1 12 -0.3354893814864184 1 13 0.06363736620185989 1 14 -0.597519683975051 1 15 -1.4953216030806047 1 16 0.13175339602519642 1 17 -0.5048303336416353 1 18 -0.7226233868152079 1 19 0.03666612375452871 1 20 -0.9851667753585147 1 21 0.3535899100638886 1 22 0.007006822148440506 1 23 -0.08749167699965454 1 24 -0.640457567487914 1 25 -0.7122092065418499 1 26 1.0016562092024728 1 27 0.6618710291305447 1 28 1.3998891465884284 1 29 0.6906173109137939 1 30 1.174909009556231 1 31 0.7483621672983958 1 32 -0.14050940264633027 1 33 -0.2044695909731189 1 34 -0.2344218248707577 1 35 -0.5525968722536305 1 36 0.441959365246241 1 37 0.1941320194707572 1 38 0.19941207350533574 1 39 1.1668052979488976 1 40 0.43086377056857117 1 41 0.27859830326293533 1 42 0.964199966426726 1 43 1.2785890153979267 1 44 0.13753570553578598 1 45 -0.30563513963879174 1 46 0.15016620961723604 1 47 -0.3152988891148283 1 48 -0.07697730131819469 1 49 1.0241281495931958 1 50 -0.47123111187014644 1 51 1.252953443433281 1 52 1.850708548311598 1 53 1.41352322513087 1 54 1.4458924141805802 1 55 1.7902939324815712 1 56 2.1895876699019254 2 1 -0.6956241989728582 2 2 -0.9457856875029762 2 3 -0.9270091407999814 2 4 -1.2420030172520171 2 5 -1.2489536779135477 2 6 -1.2938138169376572 2 7 -0.7313605905063696 2 8 -0.8378749732324238 2 9 -1.118380397066666 2 10 -1.3079029135506843 2 11 -2.203411887455303 2 12 -2.3752329712300053 2 13 -2.416277163712671 2 14 -1.64025472671009 2 15 -1.596603654362653 2 16 -0.5005115612397617 2 17 -1.6456422994535984 2 18 -0.761084925276748 2 19 -1.3161116540232527 2 20 -0.834953100144844 2 21 -2.3538887224147444 2 22 -1.9150017248601046 2 23 -1.1060814205893976 2 24 -1.3447310717614158 2 25 -2.7968245911572325 2 26 -1.1009078933616294 2 27 -1.1368469195874056 2 28 -1.7368629901637078 2 29 -1.1123741420776554 2 30 -1.394535434888212 2 31 -2.122150653214419 2 32 -1.0377316248685489 2 33 -1.9279738644773907 2 34 -1.4132679787169096 2 35 -1.3521695218262835 2 36 -0.44436542107854393 2 37 -0.2569363565976204 2 38 -0.08584433675108016 2 39 -1.521656240512641 2 40 -0.5204182807134821 2 41 0.3931282177928459 2 42 -1.1977658455390845 2 43 -0.28273577092686253 2 44 -0.24301985001977044 2 45 -0.2304214644251168 2 46 -0.3389363544853268 2 47 -0.4176493164652548 2 48 -0.9064644808053721 2 49 -1.076512876047829 2 50 -0.9945217101607433 2 51 -1.4352944198145856 2 52 -1.3922401696371196 2 53 -1.6003656637580193 2 54 -1.1143639960758271 2 55 -1.2005180333303933 2 56 0.056468011782268235 3 1 -0.33088989945595415 3 2 -0.2338291657638507 3 3 -0.3056323292057783 3 4 0.20002596825522545 3 5 0.051770959767608815 3 6 -0.03294425172026649 3 7 0.038566945725513335 3 8 0.011762707926997962 3 9 -0.022969769047342936 3 10 0.2084014342754017 3 11 -0.9141606797258319 3 12 0.005201811378690024 3 13 0.005220420828393912 3 14 -0.5049890262270011 3 15 0.42151228766633153 3 16 0.02183143392932152 3 17 0.19795673436282613 3 18 0.14471217617252563 3 19 -0.11985561537591138 3 20 0.09197926700491621 3 21 -0.9668114277287501 3 22 0.3392253282799942 3 23 -0.5934002611691085 3 24 0.007925933069500957 3 25 0.6162188871036349 3 26 0.3193819617108389 3 27 0.3740226456299851 3 28 1.2872915992082739 3 29 0.1352103989851443 3 30 0.6821553863678247 3 31 2.200313114901519 3 32 2.0015195828609222 3 33 2.1529440099187482 3 34 1.9943407169352625 3 35 2.175608255951495 3 36 2.3793060765059977 3 37 2.956227894610109 3 38 2.7746629096257323 3 39 2.0979089768786636 3 40 2.6607411395763734 3 41 2.330326285425695 3 42 1.9483694201613986 3 43 2.8652110889765225 3 44 2.676666140318396 3 45 3.0352790186666745 3 46 2.09041147160163 3 47 2.723051166626533 3 48 2.995709432238108 3 49 2.4506610369956476 3 50 2.7778212850083364 3 51 2.6293191067554638 3 52 3.283484468044037 3 53 3.51497250049319 3 54 3.2878099169676496 3 55 3.5742404207758867 3 56 1.139197480381302 4 1 -0.4468319284414619 4 2 -0.3932494556189212 4 3 -1.0447627639883876 4 4 -0.8869305534839 4 5 -1.0351855619715167 4 6 -0.6706254111405556 4 7 -0.23679537311506493 4 8 -0.1911358427976424 4 9 -0.15340455165603828 4 10 -1.0379753773188014 4 11 -0.11705923045047228 4 12 -0.023783695867692245 4 13 0.23710447879940233 4 14 0.08921387232372524 4 15 0.3780340267967617 4 16 0.41313578175540755 4 17 0.44433354595702923 4 18 0.46355275588267375 4 19 0.3149269933197445 4 20 0.22241404961361155 4 21 0.5984059635755941 4 22 0.5711093862510097 4 23 1.0442808982511806 4 24 0.6021288316202202 4 25 0.5872333798572598 4 26 -0.042936878868871986 4 27 -0.5390208326308823 4 28 -0.2779257920960774 4 29 -0.40102148507282465 4 30 0.5662133573823169 4 31 -0.7996868850984811 4 32 -0.8245673736608197 4 33 -0.28183859877690765 4 34 0.3131812966454106 4 35 -0.5490294252079195 4 36 -1.3308388510302365 4 37 -0.6959460184333679 4 38 -0.8195399889249941 4 39 -0.9745547912372778 4 40 -0.6291139328873925 4 41 0.6781523723822183 4 42 0.3686592752338633 4 43 0.4594139875272454 4 44 0.4302893287241929 4 45 0.7599166998260927 4 46 1.4672230658045287 4 47 0.9839207318439236 4 48 1.4884630554265108 4 49 2.189791471778257 4 50 0.647386502399641 4 51 2.3974350487844482 4 52 0.7907308448556343 4 53 1.9062768483192762 4 54 1.9399838300111298 4 55 2.0235157830947266 4 56 -0.06370107034333472 5 1 -0.0011797545284153443 5 2 -0.19823666789513794 5 3 -0.39514639570194277 5 4 -0.47239517411476584 5 5 0.13339926326292328 5 6 -0.014402052231773155 5 7 -0.14067431599656288 5 8 -0.08052203205595276 5 9 0.13325358986143954 5 10 -0.15839310962911668 5 11 0.08008484457084108 5 12 -0.414448657504515 5 13 0.3941803526271954 5 14 0.6456929857082088 5 15 -0.10640757593128569 5 16 -0.29381221483453146 5 17 0.42707011884705537 5 18 -0.849320219394393 5 19 -0.5603926997748871 5 20 -0.12498577988340287 5 21 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46 1.957632272965654 6 47 1.4785925136001055 6 48 1.191574734880902 6 49 2.661232221991387 6 50 2.4719815578131126 6 51 2.4658493710350875 6 52 3.139622575460919 6 53 2.9723467545426345 6 54 3.2777928666692695 6 55 1.84278262026438 6 56 0.23212160655351077 7 1 0.2185172151685535 7 2 0.30042643370914135 7 3 0.2813241925333543 7 4 -0.30260907785807944 7 5 0.34953117057130356 7 6 0.25164072851688957 7 7 0.2421242579784817 7 8 0.3931856328281853 7 9 0.3538418251555555 7 10 0.3928546622068936 7 11 0.5377497287063164 7 12 0.7081003621033233 7 13 0.07109657366106603 7 14 0.6122705258151626 7 15 0.001881194122191232 7 16 0.15226621653801686 7 17 0.451579922768623 7 18 0.37527871108688515 7 19 -0.14818236109395855 7 20 0.70923881904708 7 21 0.137272893746875 7 22 0.7529275680691896 7 23 0.5567973672893913 7 24 0.650218423188079 7 25 0.26378146944882985 7 26 0.9293951369414017 7 27 0.12830459556411 7 28 1.4651572118564928 7 29 1.1174238377203238 7 30 1.599151433798653 7 31 1.8248190437552765 7 32 1.0594905973536726 7 33 1.3927332062296784 7 34 0.6359744455255125 7 35 1.9786385589817996 7 36 1.8081598314467087 7 37 2.0675585928973277 7 38 1.8497617238549822 7 39 2.5010710322146323 7 40 2.4341271738319747 7 41 2.50423932890396 7 42 2.425971528198289 7 43 2.6478197846286946 7 44 2.816323584323662 7 45 2.9918007577971046 7 46 1.8315181909692164 7 47 2.0583607845448455 7 48 3.282929458588569 7 49 2.4348507603158076 7 50 3.4234075827685473 7 51 2.9573823478621826 7 52 3.429729527332576 7 53 1.9741292857369324 7 54 2.499272367560536 7 55 3.555795097982738 7 56 1.8658114461257043 8 1 -0.35117975452841677 8 2 -0.2541190208363133 8 3 0.16593957714874108 8 4 0.15543288018388068 8 5 -0.006645985605857163 8 6 -0.38612150924534916 8 7 -0.04995033409610983 8 8 -0.05133651169396103 8 9 0.1384572097709409 8 10 0.011968881321109848 8 11 0.13868212963871684 8 12 0.5875875415905085 8 13 1.0174835200480103 8 14 0.24863416217879575 8 15 0.5046783969193953 8 16 0.6702149344867365 8 17 0.464400435589134 8 18 0.5696843054924798 8 19 -0.05564156855316327 8 20 0.6917563015645598 8 21 0.7689745254485061 8 22 1.1762375913792127 8 23 1.312508323000344 8 24 0.9441578171274685 8 25 0.3339446396119996 8 26 1.4631946707409398 8 27 1.1080248752843929 8 28 1.261427608126887 8 29 1.1060019262984113 8 30 2.19029362494085 8 31 1.0868237057599401 8 32 1.5210290588921325 8 33 0.995530409026884 8 34 1.888655098206165 8 35 3.1550954354386747 8 36 2.703497826784705 8 37 2.3633627887015223 8 38 2.353258227351489 8 39 2.5514206825642844 8 40 2.430863770568571 8 41 3.6016752263398537 8 42 3.7334307356574996 8 43 3.047819784628693 8 44 3.368304936305016 8 45 4.2174417834381295 8 46 5.257858517309547 8 47 4.62316264934671 8 48 4.523022698681807 8 49 4.9318204572855 8 50 5.390307349668316 8 51 5.7452611357409715 8 52 5.235323932926985 8 53 6.936600148207791 8 54 4.399738568026734 8 55 6.57490931709696 8 56 4.697279977594235 9 1 0.1741933797999451 9 2 0.15185112841741955 9 3 0.10968240148857689 9 4 -0.0305602311823705 9 5 0.24731497880286923 9 6 0.18379812336085166 9 7 -0.05660934672527418 9 8 0.21249816109593667 9 9 0.12996122814062971 9 10 0.15043041978265137 9 11 1.1189347128763742 9 12 0.5862098148396413 9 13 0.9290793868677625 9 14 0.9650520726265555 9 15 0.6931973406622234 9 16 0.7244055200206034 9 17 1.1555600222711107 9 18 0.5150344777083262 9 19 0.8515914968888509 9 20 0.32321439570922195 9 21 0.49457728090200703 9 22 0.4765820230669249 9 23 0.9281225595101006 9 24 0.932217518620007 9 25 1.7181007819770926 9 26 1.4861567832552893 9 27 0.32708342867130114 9 28 1.8120590662210319 9 29 1.8001465876072515 9 30 1.9808791588099623 9 31 1.9437697447955316 9 32 2.0594905973536726 9 33 1.6736015915756752 9 34 1.5699409765069703 9 35 1.5984938280908025 9 36 1.3723841643277623 9 37 1.31789780592311 9 38 1.555324817477775 9 39 1.228343759487359 9 40 1.0641588337143801 9 41 1.3947865925855503 9 42 1.1127648343945822 9 43 1.319461575673472 9 44 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2.9166897071695423 120 52 2.166093163696214 120 53 3.356380367988013 120 54 2.640397908686076 120 55 2.7133708555584946 120 56 0.6632140435283009 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 27.0 5 1.0 6 16.0 7 10.0 8 35.0 9 35.0 10 43.0 11 94.0 12 100.0 13 131.0 14 160.0 15 236.0 16 343.0 17 464.0 18 552.0 19 611.0 20 619.0 21 790.0 22 742.0 23 861.0 24 1049.0 25 1166.0 26 1263.0 27 1473.0 28 1595.0 29 1881.0 30 2194.0 31 2576.0 32 3148.0 33 3702.0 34 4967.0 35 6787.0 36 9117.0 37 11517.0 38 11652.0 39 6196.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.38282142528855 0.5173794860346933 0.03414179349467519 0.06565729518206767 8 99.61905762738581 0.3008131149263731 0.04072142603806797 0.03940783164974319 9 97.00077475608315 1.6217351909970719 0.5672790303730648 0.8102110225467151 10 58.79216839783068 25.627355455464656 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20.106890076556404 23.610363347471537 25.184825285937524 24 30.031781052189217 21.294618233393745 23.75567987812886 24.917920836288182 25 29.30862381465234 19.363524127242638 23.134308755155114 28.19354330294991 26 27.00373153939139 18.592000840910288 25.01708099017186 29.38718662952646 27 26.205963127948383 18.108829056122786 25.08902642540834 30.596181390520492 28 26.352177284736005 20.99472930166533 23.76546049605026 28.8876329175484 29 29.090885180363223 18.924001525492827 25.5598953196302 26.42521797451375 30 27.41882411782513 18.766205562209617 28.498098108638143 25.316872211327112 31 29.493105764292203 19.99657592877932 26.64059104737071 23.86972725955777 32 33.02956475933364 18.031210904062686 27.293079607559097 21.64614472904458 33 30.209279966283404 18.048915405586946 28.35091601143203 23.390888616697616 34 25.782269008313463 21.84292697065915 32.43567278428479 19.9391312367426 35 25.583234480031635 23.930407275603006 31.876894688282587 18.609463556082773 36 27.476859787452863 23.537090266606896 27.30545080562222 21.68059914031803 37 27.729940001318653 23.243884749785718 27.99894507813015 21.027230170765478 38 28.146105951878432 22.018784297171802 28.569544111439427 21.265565639510342 39 29.13769215543973 19.940621495018803 28.78010160321963 22.141584746321833 40 27.063856758041517 19.542597580943326 30.852215708023028 22.541329952992132 41 24.655616456448524 19.74245525985604 29.953113649871227 25.64881463382421 42 24.134696681462692 19.43431621221729 29.927605157471994 26.503381948848027 43 24.475663102805715 18.685820767309394 32.45536363275933 24.38315249712556 44 23.777868819961146 18.19947929744803 31.702062986506668 26.320588896084157 45 25.619670830854652 18.758675391631964 31.408553109921343 24.21310066759204 46 24.92759756145942 19.67230457953689 30.504238352794932 24.895859506208755 47 24.839930151338766 19.58408297174304 31.28505661974812 24.29093025717007 48 25.97813426514189 19.21590427784837 31.535895806861497 23.27006565014824 49 25.532646957626447 20.9843051107641 30.511076410025673 22.971971521583782 50 24.667328699106257 19.269116186693147 31.330023171135384 24.73353194306521 51 24.973199041783026 21.160183702370396 30.150747118069805 23.71587013777678 52 24.67991579170363 19.273902048274127 31.043203092933652 25.002979067088592 53 24.566072208761966 19.479419046484225 31.245448888535833 24.709059856217976 54 25.210498925821646 20.613549325469368 30.130369223789383 24.045582524919602 55 24.20662251655629 20.018543046357614 27.536423841059605 28.23841059602649 56 25.89752757803292 20.28657317283117 27.454875319481413 26.361023929654497 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 0.5 11 0.0 12 6.0 13 19.5 14 27.0 15 64.5 16 102.0 17 130.0 18 158.0 19 185.5 20 213.0 21 286.5 22 434.0 23 508.0 24 477.0 25 446.0 26 549.0 27 652.0 28 763.0 29 1028.0 30 1182.0 31 1342.0 32 1502.0 33 1589.5 34 1677.0 35 1701.0 36 1725.0 37 1402.5 38 1139.0 39 1198.0 40 997.0 41 796.0 42 805.0 43 814.0 44 832.0 45 850.0 46 882.5 47 1163.0 48 1411.0 49 1829.0 50 2247.0 51 1955.0 52 1663.0 53 1704.5 54 2105.5 55 2465.0 56 2669.5 57 2874.0 58 3143.0 59 3412.0 60 3903.0 61 4394.0 62 5201.0 63 6267.0 64 6526.0 65 6557.5 66 6589.0 67 5848.5 68 5108.0 69 4719.0 70 4330.0 71 4049.5 72 3229.5 73 2690.0 74 2266.0 75 1842.0 76 1632.0 77 1422.0 78 1303.0 79 909.5 80 635.0 81 486.5 82 338.0 83 268.5 84 199.0 85 160.0 86 121.0 87 85.0 88 37.5 89 26.0 90 16.0 91 6.0 92 4.5 93 3.0 94 3.0 95 3.0 96 1.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.03414179349467519 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0013131459036413535 21 0.0 22 0.0 23 0.0 24 0.009192021325489476 25 0.019697188554620305 26 0.05909156566386091 27 0.06828358698935039 28 0.09454650506217746 29 0.1470723412078316 30 0.23111367904087823 31 0.2888920988010978 32 0.2862658069938151 33 0.2967709742229459 34 0.3309127677176211 35 0.37162029073050307 36 0.408388376032461 37 0.4175803973579505 38 0.45303533675626695 39 0.48323769254001814 40 0.5528344254330099 41 0.5751579057949129 42 0.6001076779640986 43 0.6381889091696978 44 0.6381889091696978 45 0.6670781190498076 46 0.7012199125444828 47 0.7366748519427994 48 0.7892006880884536 49 0.7708166454374746 50 0.82465562748677 51 0.7826349585702467 52 0.8220293356794873 53 0.816776752064922 54 1.5915328352133205 55 0.857484275077804 56 0.8404133783304664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 76153.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.317439890746265 #Duplication Level Percentage of deduplicated Percentage of total 1 69.54632905107677 29.430225992410016 2 12.23856513374294 10.358094887922997 3 6.0106746105629 7.630690846059905 4 3.295475702848632 5.5782437986684705 5 2.147334450443741 4.543484826599084 6 1.4801712902625208 3.758223576221554 7 0.9929870291069324 2.9414468241566323 8 0.7354310184323217 2.4897246333040064 9 0.5554521194066903 2.1154780507662205 >10 2.736920498975982 20.116082097881897 >50 0.20170049028734563 5.857943876144079 >100 0.05585552038726494 4.2637847491234755 >500 0.003103084465959163 0.9165758407416649 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCGACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 698 0.9165758407416649 No Hit CCGACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 367 0.4819245466363768 No Hit CCGACGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 289 0.3794991661523512 No Hit CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 272 0.35717568579044817 No Hit CCGACGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 253 0.33222591362126247 No Hit CCGACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 248 0.3256601841030557 No Hit CCGACGGGGCTATCACCACAGCTTTTTCTCCATAAGGCTTCTCACACCTCACACCC 232 0.304649849644794 No Hit CCGACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 165 0.21666907410082334 No Hit CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 164 0.21535592819718197 No Hit CCGACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT 157 0.2061639068716925 No Hit CCGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 148 0.19434559373892032 No Hit CCGACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 139 0.18252728060614815 No Hit CCGACGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 128 0.16808267566609325 No Hit CCGACGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 125 0.1641432379551692 No Hit CCGACGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 121 0.1588906543406038 No Hit CCGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 116 0.152324924822397 No Hit CCGACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 114 0.1496986330151143 No Hit CCGACGGGGGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTCTGACATG 107 0.14050661168962483 No Hit CCGACGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG 102 0.13394088217141806 No Hit CCGACGGGGGGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCGCCTAGCTCT 97 0.12737515265321128 No Hit CCGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG 94 0.12343571494228725 No Hit CCGACGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 93 0.12212256903864588 No Hit CCGACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGT 92 0.12080942313500453 No Hit CCGACGGGGGGTGGCATCTGGTATCTGGATGCTCCACCCTAAAGTGGGAGACCTCA 91 0.11949627723136318 No Hit CCGACGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 89 0.11686998542408046 No Hit CCGACGGGGGGGGCTGGAGAGATGGCTCCAGTGGTTAAGAGCACTGACTGCTCTTC 86 0.1129305477131564 No Hit CCGACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 86 0.1129305477131564 No Hit CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC 85 0.11161740180951506 No Hit CCGACGGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA 84 0.1103042559058737 No Hit CCGACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG 83 0.10899111000223236 No Hit CCGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 82 0.10767796409859098 No Hit CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 82 0.10767796409859098 No Hit CCGACGGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG 81 0.10636481819494964 No Hit CCGACGGGGAAGACCTTGCCCCTCCAGACCTGGGCCGAGACGACAAGGGGAGCCGC 81 0.10636481819494964 No Hit CCGACGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC 80 0.1050516722913083 No Hit CCGACGGGGGAGAGAAGGCCACTGGCCGCTATTATGCCATGAAGATCCTGCGCAAG 79 0.10373852638766692 No Hit CCGACGGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCT 77 0.10111223458038424 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.002626291807282707 0.0 0.0 0.0 0.0 10 0.006565729518206768 0.0 0.0 0.0 0.0 11 0.010505167229130828 0.0 0.0 0.0 0.0 12 0.011818313132772183 0.0 0.0 0.0 0.0 13 0.013131459036413535 0.0 0.0 0.0 0.0 14 0.01444460494005489 0.0 0.0 0.0 0.0 15 0.015757750843696242 0.0 0.0 0.0 0.0 16 0.015757750843696242 0.0 0.0 0.0 0.0 17 0.015757750843696242 0.0 0.0 0.0 0.0 18 0.015757750843696242 0.0 0.0 0.0 0.0 19 0.015757750843696242 0.0 0.0 0.0 0.0 20 0.01838404265097895 0.0 0.0 0.0 0.0 21 0.019697188554620305 0.0 0.0 0.0 0.0 22 0.021010334458261656 0.0 0.0 0.0 0.0 23 0.023636626265544365 0.0 0.0 0.0 0.0 24 0.02626291807282707 0.0 0.0 0.0 0.0 25 0.02626291807282707 0.0 0.0 0.0 0.0 26 0.02626291807282707 0.0 0.0 0.0 0.0 27 0.030202355783751134 0.0 0.0 0.0 0.0 28 0.04464696072380602 0.0 0.0 0.0 0.0 29 0.04858639843473008 0.0 0.0 0.0 0.0 30 0.05252583614565414 0.0 0.0 0.0 0.0 31 0.0538389820492955 0.0 0.0 0.0 0.0 32 0.05777841976021956 0.0 0.0 0.0 0.0 33 0.05909156566386091 0.0 0.0 0.0 0.0 34 0.06303100337478497 0.0 0.0 0.0 0.0 35 0.07222302470027445 0.0 0.0 0.0 0.0 36 0.0761624624111985 0.0 0.0 0.0 0.0 37 0.08010190012212257 0.0 0.0 0.0 0.0 38 0.08404133783304663 0.0 0.0 0.0 0.0 39 0.08666762964032934 0.0 0.0 0.0 0.0 40 0.0906070673512534 0.0 0.0 0.0 0.0 41 0.09454650506217746 0.0 0.0 0.0 0.0 42 0.09585965096581882 0.0 0.0 0.0 0.0 43 0.09848594277310152 0.0 0.0 0.0 0.0 44 0.107677964098591 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTTAT 20 3.925785E-4 50.58761 50 CTAGTTA 20 3.925785E-4 50.58761 49 AATATCA 15 0.00802141 50.587605 49 ATATCAG 15 0.00802141 50.587605 50 ACAGAGA 15 0.00802141 50.587605 50 GCTCTAG 20 4.09666E-4 50.15324 46 ACACCTC 25 2.0317635E-5 50.153233 44 CTATATA 15 0.008299813 50.153233 46 GCGCCTA 15 0.008299813 50.153233 45 ACCTCAC 25 2.0317635E-5 50.153233 46 TAGCAAG 15 0.008299813 50.153233 47 ACAAGCC 15 0.008299813 50.153233 45 TACAAGC 15 0.008299813 50.153233 44 CCTACAA 15 0.008321522 50.120132 42 ATACGCT 20 4.1100453E-4 50.120132 42 AGTATAC 20 4.123465E-4 50.087074 39 TATGGTG 15 0.008343273 50.08707 41 TTCGTCA 25 2.0477168E-5 50.08707 40 CACCTAC 15 0.008343273 50.08707 40 GTTCGTC 30 1.022514E-6 50.08707 39 >>END_MODULE