FastQCFastQC Report
Fri 17 Jun 2016
SRR1526726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458461
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT123192.6870333572539433No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC13160.28704731700188235No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11070.24146001513760165No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC9590.2091780980279675No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7560.16489952253299628No Hit
GATCTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6300.13741626877749688No Hit
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT5870.12803706313077884No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAGCAGTGGTATCAA5470.11931222066871555No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC5470.11931222066871555No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.10557059379096587No Hit
GATCTTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT4730.10317126211389846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTC150.00468070758.0147748
ATACGCT351.6449121E-857.97037534
TGGGTAC29750.056.884116
GGTACCT28750.056.198658
TAGCATA25300.055.9860430
ATTAAGC21200.055.96848351
GTACCTG28850.055.838949
CCAGTAG26450.055.83334426
GCCAGTA26500.055.82507325
CATGCAT17400.055.81755458
GGGTACC29250.055.768887
GTAGCAT25800.055.56860729
TACCTGG29000.055.45038610
GCATATG25350.055.4306632
GGTTGAT28600.055.3701415
ATATGCT25300.055.3353634
TTGATCC28500.055.2596817
AGTAGCA25950.055.2413628
ACCTGGT29250.055.2284211
TATGCTT25150.055.21646535
Produced by FastQC (version 0.11.5)