FastQCFastQC Report
Fri 17 Jun 2016
SRR1526725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606269
Sequences flagged as poor quality0
Sequence length64
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT285144.70319280715326No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT32730.539859369355847No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC27970.461346366052033No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC26860.44303766150009316No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC25170.4151622464615542No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16670.2749604548476006No Hit
GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT13260.21871479491776752No Hit
GATCTTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT12760.21046763070518204No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC11600.19133420973198367No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10990.18127266939262934No Hit
GATCTTGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG10980.18110772610837764No Hit
GATCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG10440.17220078875878528No Hit
GATCTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG9700.15999498572415874No Hit
GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC9340.15405702749109718No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT8390.13838741548718472No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC8290.13673798264466763No Hit
GATCTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8240.13591326622340907No Hit
GATCTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG8160.1345937199493954No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC8040.1326144005383749No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT7740.12766610201082357No Hit
GATCTTGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG7000.11546029897619703No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6880.1134809795651765No Hit
GATCTTGGGGGGGCTGGAGAAATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCC6350.10473898549983589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGC202.00152E-458.0473237
ATATTGC150.0046817758.01372534
ACGCTTA303.7826794E-757.94664427
TGGGTAC36050.056.3113486
TACCTGG34200.055.41204510
GGTACCT34600.055.2396628
TAGCATA32600.055.2188830
CATGCAT30350.055.2061258
CATATGC32450.055.05932233
ATATGCT32300.055.05017534
GGTTGAT34200.054.9890515
ATTAAGC30900.054.944751
GTACCTG34650.054.9429059
GTTGATC34100.054.81092516
GTAGCAT33000.054.80855629
GGGTACC35300.054.718517
ACCTGGT34750.054.61828211
GCATATG32800.054.55120532
TATGCTT32400.054.540235
TGGTTGA34600.054.5205814
Produced by FastQC (version 0.11.5)