FastQCFastQC Report
Fri 17 Jun 2016
SRR1526723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556287
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG307285.52376740783085No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC46970.844348330987422No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC35460.6374407455144557No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC32580.585668908315312No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT18280.3286073555556754No Hit
GATCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT15620.2807903114759108No Hit
GATCTTGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC11470.20618853217853372No Hit
GATCTTGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC9770.17562876716515036No Hit
GATCTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA9560.17185373736937948No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC9080.16322509783618888No Hit
GATCTTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC8320.14956308524197043No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8310.1493833219183623No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC8080.1452487654753751No Hit
GATCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC7830.1407546823851717No Hit
GATCTTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC7490.13464272938249502No Hit
GATCTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA7220.1297891196450753No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT7110.12781172308538577No Hit
GATCTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC6860.12331763999518235No Hit
GATCTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA6640.11936284687580333No Hit
GATCTTGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT6280.11289136722591037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT251.9274994E-550.6725950
TATCGGG203.9151384E-450.67258550
CGGGTAT150.00831198750.17669739
CGACGTA150.00844977749.9684227
TCCGACG204.1950593E-449.9684227
ACGACGT150.00846183949.9503926
AACGACG150.00846787449.9413825
ACAAACG204.240079E-449.86043523
TATGTAT150.00854662849.82454722
TCATATA150.00857096949.78870819
CAACGAC252.1423964E-549.77528414
CGGCAAT150.0085801149.775289
GTCTACG204.2759912E-449.7752810
TGAATCG204.2759912E-449.7752817
AGTCGTG150.0085801149.7752813
TGGGTAC42350.048.4584966
GATTAAG37150.048.4219850
TATGCTT37900.048.2838335
ATATGCT37850.048.2682734
AGATTAA37300.048.22725749
Produced by FastQC (version 0.11.5)