FastQCFastQC Report
Fri 17 Jun 2016
SRR1526722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145026
Sequences flagged as poor quality0
Sequence length64
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39402.716754237171266No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12310.8488133162329513No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9160.6316108835657055No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT8980.61919931598472No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.6026505592100727No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8020.5530042888861307No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7180.49508364017486517No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5260.36269358597768675No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.3371809192834388No Hit
TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4050.2792602705721733No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3230.22271868492546162No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3110.21444430653813798No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.17583054073062762No Hit
TAGTCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2450.1689352254078579No Hit
TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.15859225242370334No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.10825645056748445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACTC150.004646728758.1012853
TAACTCC150.004646728758.1012854
GATAAGC150.004659509858.06100543
AGGAAAG150.004659509858.06100542
CGGGTAT202.0009148E-458.020785
CGGGATA202.0009148E-458.020785
CAGCGTA150.004678730458.0006936
AGCGTAG150.004678730458.0006937
TAACGAT150.004678730458.0006937
GCGCAAC150.004678730458.0006937
CGTAGGT150.004678730458.0006932
TACTATA150.004678730458.0006934
ATAACGA150.004678730458.0006936
TCATAAC150.004678730458.0006934
GCGTAGA150.004678730458.0006938
TCGTGAT150.004691576657.96055228
TACCCCG150.00472380857.8604524
CCCTATA202.028489E-457.8604515
CCGTCGC150.00472380857.8604515
ATTCAGG150.00472380857.8604515
Produced by FastQC (version 0.11.5)