FastQCFastQC Report
Fri 17 Jun 2016
SRR1526721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188408
Sequences flagged as poor quality0
Sequence length64
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56653.006772536198038No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT21561.144325081737506No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17420.9245891894187083No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.7834062247887564No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12670.6724767525795083No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11700.6209927391618191No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9790.5196170014012144No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7390.39223387541930277No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7360.3906415863445289No Hit
TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5960.31633476285508044No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530.24043565029085814No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4220.2239819965181946No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.1709056940257314No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3160.1677211158761836No Hit
TAGTCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3130.16612882680140972No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.12154473270774066No Hit
TAGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG2210.1172986285083436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTTA201.9708344E-458.2079658
ATAATTC150.004629916558.1613852
CGGCTCT150.004639706658.13036747
CAATCTA201.9838782E-458.13036748
TTAGATA150.00464460758.11487646
CGACAAT201.9943625E-458.06844345
CGAGCAT407.057679E-1058.06844343
TAACGAT150.004664248358.05298637
TCCGTCA150.004664248358.05298638
CGTCATC150.004664248358.05298640
TAATGAC150.004664248358.05298638
CAACGAC201.9969905E-458.05298242
TCATAAC150.004679019658.00665334
CGGTAGG258.649289E-657.99122231
CCGTGAA150.004688886557.97580330
AAGGGTA150.004688886557.97580329
GACGTGA150.004698768757.94499228
GTGACTA150.00470371657.92959626
CCGAGAT150.00470866757.9142121
ACCGCAA202.0207663E-457.91420724
Produced by FastQC (version 0.11.5)