FastQCFastQC Report
Fri 17 Jun 2016
SRR1526720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188370
Sequences flagged as poor quality0
Sequence length64
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53912.8619206880076447No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT20351.0803206455380368No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17100.9077878643096035No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14660.7782555608642565No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11700.6211180124223602No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10840.5754631841588363No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8890.47194351542177626No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7450.3954982215851781No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6870.3647077560121038No Hit
TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5980.31746031746031744No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4260.22615066093326963No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3390.17996496257365824No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3150.16722408026755853No Hit
TAGTCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.15766841853798377No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2910.15448319796145882No Hit
TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.11095185008228485No Hit
TAGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG2000.10617401921749747No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.1008653182566226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC150.00453901958.45322845
AACGATA150.004543853558.43751544
GCTAGTT150.004543853558.43751544
GACCATT150.00455353458.40612440
CATTTGA150.00455353458.40612443
CACGTCT201.9379708E-458.4061240
TTGTTAC150.004563229658.37476323
TTCCTAT150.004563229658.37476321
CTCGTGA150.00466103758.0630258
TAGACTC201.9952706E-458.06301557
ATTAGTA258.626837E-658.0165454
TAATCAC150.004680784458.0010750
CGTGCGT150.004680784458.0010747
TCATTAG258.640496E-658.0010752
ATGCATC150.004680784458.0010746
TTAACTC150.004685730757.985653
CAACTAT258.709059E-657.92381738
ACACAAT150.004705555757.92381338
ATGCTCC150.004705555757.92381337
TTGCGAT150.00471052257.9083935
Produced by FastQC (version 0.11.5)