FastQCFastQC Report
Fri 17 Jun 2016
SRR1526719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165547
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44112.6645001117507414No Hit
TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG23751.4346378973946976No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA17201.0389798667447916No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT15390.929645357511764No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA11430.6904383649356376No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10200.6161392233021438No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA9730.5877484943852803No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6170.3727038242915909No Hit
TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6070.3666632436709817No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5850.3533739663056413No Hit
TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4100.24766380544497937No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3700.22350148296254235No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3390.20477568303865368No Hit
TAGTCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3150.1902782895491915No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.17578089605972927No Hit
TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2300.13893335427401282No Hit
TAGTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2110.12745625109485526No Hit
TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.12081161241218506No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.11235479954333209No Hit
TAGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA1860.11235479954333209No Hit
TAGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC1740.105106102798601No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA1680.10148175442623544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACCC203.9251635E-450.62495849
GATAATC150.00802451450.60944448
GCGATAA150.00829038850.1939846
TACTATT150.00829038850.1939846
ATAGTAA150.00829038850.1939845
TGCGTTC150.00829038850.1939845
TAGTAAT150.00829038850.1939846
ATATAAG150.00830035950.17872644
TATAGTA150.00830035950.17872644
CCGTGAT204.100541E-450.17872244
TGGTTAC150.00832032950.1482442
TTGCAAT150.00832032950.1482442
ATGCTAG204.1128616E-450.14823542
TCATCTC204.1128616E-450.14823542
AAACTGG150.00836037550.0873839
TAGCAGG204.1375912E-450.08737639
TAACGAT204.143792E-450.07218637
TGATGCT204.143792E-450.07218638
GTTTAAC150.00839050350.04183236
AATCTCT150.00839050350.04183236
Produced by FastQC (version 0.11.5)