##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526719_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 165547 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.34890393664639 39.0 38.0 40.0 35.0 40.0 2 37.833618247385935 39.0 38.0 40.0 35.0 40.0 3 37.84004542516627 39.0 38.0 40.0 35.0 40.0 4 37.18962590684217 39.0 37.0 40.0 33.0 40.0 5 37.987260415471134 39.0 38.0 40.0 35.0 40.0 6 37.82522788090391 39.0 38.0 40.0 35.0 40.0 7 38.13039801385709 39.0 38.0 40.0 35.0 40.0 8 38.02183065836288 39.0 38.0 40.0 35.0 40.0 9 37.7889662754384 39.0 38.0 40.0 35.0 40.0 10 36.96680700948975 39.0 36.0 40.0 32.0 40.0 11 36.98544824128495 39.0 36.0 40.0 32.0 40.0 12 36.98292931916616 39.0 36.0 40.0 32.0 40.0 13 36.757126374987166 39.0 36.0 40.0 32.0 40.0 14 37.182298682549366 39.0 37.0 40.0 33.0 40.0 15 37.085613149135895 39.0 36.0 40.0 33.0 40.0 16 36.84212338489976 39.0 36.0 40.0 32.0 40.0 17 36.88332014473231 39.0 36.0 40.0 32.0 40.0 18 36.70860843144243 39.0 36.0 40.0 32.0 40.0 19 36.702006076824105 39.0 36.0 40.0 32.0 40.0 20 36.69449159453207 39.0 36.0 40.0 32.0 40.0 21 36.617866829359635 39.0 36.0 40.0 32.0 40.0 22 36.4826182292642 38.0 36.0 40.0 31.0 40.0 23 36.2074274979311 38.0 35.0 40.0 31.0 40.0 24 36.155647640851235 38.0 35.0 40.0 31.0 40.0 25 35.89868134125052 38.0 35.0 40.0 30.0 40.0 26 35.661830175116435 38.0 35.0 40.0 30.0 40.0 27 35.5482733000296 38.0 35.0 40.0 29.0 40.0 28 35.21402381196881 38.0 35.0 39.0 29.0 40.0 29 35.08682126526001 38.0 35.0 39.0 28.0 40.0 30 34.672769666620354 38.0 34.0 39.0 27.0 40.0 31 34.103698647514 38.0 33.0 39.0 24.0 40.0 32 33.85916386283049 38.0 33.0 39.0 23.0 40.0 33 33.77057874802926 38.0 33.0 39.0 23.0 40.0 34 33.75428730209548 38.0 33.0 39.0 23.0 40.0 35 33.36389061716612 37.0 33.0 39.0 20.0 40.0 36 33.118171878680975 38.0 33.0 39.0 11.0 40.0 37 32.875678810247244 38.0 33.0 39.0 10.0 40.0 38 32.67307169565139 37.0 32.0 39.0 8.0 40.0 39 32.46468374540161 37.0 32.0 39.0 4.0 40.0 40 32.231771037832154 37.0 32.0 39.0 4.0 40.0 41 31.84077029484074 37.0 32.0 39.0 4.0 40.0 42 31.66341280723903 36.0 31.0 39.0 4.0 40.0 43 31.46619389055676 36.0 31.0 39.0 4.0 40.0 44 31.29146405552502 36.0 31.0 39.0 4.0 40.0 45 30.994793019505035 36.0 30.0 39.0 4.0 40.0 46 30.47461445994189 36.0 30.0 39.0 4.0 40.0 47 30.251294194397964 36.0 29.0 39.0 4.0 40.0 48 29.972799265465397 35.0 29.0 39.0 4.0 40.0 49 29.675729551124455 35.0 28.0 39.0 4.0 40.0 50 29.355578778232164 35.0 27.0 39.0 4.0 40.0 51 28.50458178040073 35.0 24.0 39.0 4.0 40.0 52 28.22684192404574 35.0 23.0 39.0 4.0 40.0 53 27.84287241689671 35.0 18.0 39.0 4.0 40.0 54 27.330909046977595 34.0 4.0 39.0 4.0 40.0 55 26.921635547608837 34.0 4.0 39.0 4.0 40.0 56 15.776389786586286 19.0 4.0 23.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.6990339124202904 1 2 -0.8217062093312961 1 3 -0.8372778589679726 1 4 -1.2804006558436356 1 5 -1.133281240986463 1 6 -1.0892966334891767 1 7 -1.1755477311590141 1 8 -1.2942436574778569 1 9 -1.4321857619303984 1 10 -1.0868541450039686 1 11 -1.4870446408972242 1 12 -1.6999693766251056 1 13 -1.0916280929885929 1 14 -1.3829065620287793 1 15 -1.7542354782627854 1 16 -1.4611229229705387 1 17 -1.318544222479865 1 18 -1.9697288356706437 1 19 -1.368037771559024 1 20 -1.5651036632677418 1 21 -1.29365146622672 1 22 -1.7440923953933805 1 23 -1.4445073535617823 1 24 -1.6246480741142477 1 25 -2.4215231155360897 1 26 -1.5554104304260221 1 27 -2.278101743082793 1 28 -1.7366326367691798 1 29 -1.414436444906542 1 30 -1.999454931720706 1 31 -2.032446366885658 1 32 -1.7432760791979192 1 33 -2.287358688348398 1 34 -2.4101362861153355 1 35 -2.8311029416820865 1 36 -1.3091290657710921 1 37 -0.9540803136131366 1 38 -0.7285091118233744 1 39 -1.1163148150484794 1 40 -0.9994369559637128 1 41 -0.2996214400452679 1 42 -0.6959118057352462 1 43 -0.2566646695106094 1 44 -0.05044441922687426 1 45 -0.479055369763973 1 46 -0.9273450271461989 1 47 0.46444631070004405 1 48 -0.3495447884844616 1 49 0.061522360194942394 1 50 0.17419846116029092 1 51 0.5641568631299236 1 52 0.6402187160250818 1 53 0.1525851515507881 1 54 0.736634291364215 1 55 0.6065221934827854 1 56 1.3883871591823311 2 1 -0.30250587445351584 2 2 -0.37801110921056846 2 3 -0.3102806481940661 2 4 -0.35650073494109336 2 5 -0.29705460595877753 2 6 -0.4217141015379653 2 7 -0.2903639336485071 2 8 -0.16119340977792973 2 9 -0.23231897869905538 2 10 0.01755449743389903 2 11 -1.105295338203753 2 12 -0.7963017524629166 2 13 -0.6570500154730823 2 14 -0.5384787696804167 2 15 -0.2540065119416468 2 16 -0.14040355241976954 2 17 -0.25022899830102574 2 18 -0.21230824535383164 2 19 -0.30043026146369556 2 20 -0.07278444258584216 2 21 0.09176129760492557 2 22 0.019031537831715184 2 23 0.22911156321936943 2 24 0.4168572753716191 2 25 -0.3656137029387807 2 26 0.16537554850907554 2 27 0.5659097468635039 2 28 0.4404624050692121 2 29 -0.09617325852795489 2 30 0.46043433184034654 2 31 0.3913078481761616 2 32 0.24065464808237635 2 33 0.03473448012918112 2 34 0.37018093631503746 2 35 0.4475698862601263 2 36 0.5166900092049715 2 37 0.7349834222846212 2 38 0.6615246086711934 2 39 0.6060114827742638 2 40 0.3490914150449882 2 41 0.37245715784824185 2 42 0.3476750557609414 2 43 0.2588009647261025 2 44 0.6177209360872276 2 45 0.21313063414926958 2 46 -0.401263681656836 2 47 -0.33781004831914885 2 48 -0.506657315023741 2 49 0.09953076950346329 2 50 -0.16966732294527986 2 51 0.07237883557070646 2 52 0.2551806243552939 2 53 -0.19740027786513892 2 54 -0.3007496643412111 2 55 -0.1665929973918665 2 56 -0.10622172471092917 3 1 -0.3418301613546504 3 2 -0.3600896786610619 3 3 -0.3187864044151354 3 4 -0.5033536051392957 3 5 -0.5582802260877457 3 6 -0.40221020471476265 3 7 -0.29388492110746256 3 8 -0.15897795699476802 3 9 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4 36 1.0155599496200125 4 37 1.2916713203737942 4 38 0.814185385330326 4 39 0.820476245461748 4 40 0.538366122256825 4 41 0.6343022369524114 4 42 0.7551950886354071 4 43 0.7527808291189544 4 44 0.8879490026580079 4 45 1.0940839935082174 4 46 0.722960686537121 4 47 0.7194326062935481 4 48 0.4870143513004841 4 49 0.8130298449092557 4 50 1.0839180479197275 4 51 1.8667079911079973 4 52 1.8613445857277995 4 53 2.178560272782075 4 54 1.795120481539211 4 55 1.4504606290720723 4 56 1.050794917984776 5 1 0.26404222712643843 5 2 0.3660537431345361 5 3 0.29216785871304296 5 4 0.4404634473734106 5 5 0.32173106696702547 5 6 0.3730580053360555 5 7 0.538695541907785 5 8 0.6162902530560501 5 9 0.4547932721074588 5 10 0.8618344213316576 5 11 0.24336873657497193 5 12 0.2852186379746726 5 13 0.4558211897548077 5 14 0.43620896296909706 5 15 0.7109202810106225 5 16 0.34789159192632724 5 17 0.5868543005657187 5 18 0.1421462173808834 5 19 0.8245180386438449 5 20 0.7129712030424145 5 21 0.5350982154641386 5 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1.2446342191967759 6 48 0.41503854484886915 6 49 0.8533524255544194 6 50 0.7274664350165025 6 51 1.2743692814305767 6 52 1.3024736179858643 6 53 1.209407046975624 6 54 1.4068140299263057 6 55 1.0305009516527193 6 56 -0.1312615336281251 7 1 -0.11434068028872701 7 2 -0.06796385217338496 7 3 -0.09618572318893115 7 4 0.0681551105489433 7 5 -0.0033213000208291987 7 6 -0.1899080608558208 7 7 -0.4014385175811128 7 8 -0.42040853202983186 7 9 -0.4954790362293622 7 10 -0.3630084777047884 7 11 -0.7775780585653678 7 12 -0.955208676646194 7 13 -0.7263740343759011 7 14 -0.8004060349362234 7 15 -0.4815388727388523 7 16 -0.8664360159496667 7 17 -0.7065265121699369 7 18 -0.21839002057470935 7 19 -0.30153977451074354 7 20 -0.025445219244971895 7 21 -0.18232951809262943 7 22 4.5746756255482524E-4 7 23 -0.4356100311919704 7 24 -0.29072439711656983 7 25 -0.4415948001239016 7 26 0.3064069847120763 7 27 -0.11516689172289318 7 28 0.1466058199038116 7 29 -0.06292896019222383 7 30 0.617327695308596 7 31 1.0757130259037169 7 32 0.9260049665537124 7 33 0.5564110776225064 7 34 1.2302179200832768 7 35 0.5035797485983267 7 36 0.7833018851038815 7 37 0.6594132558576646 7 38 0.8012821595238719 7 39 1.4720891486875516 7 40 0.8157854770955311 7 41 0.4536570756620897 7 42 1.0648725079902412 7 43 0.35278082911895225 7 44 0.06859416394833318 7 45 0.752148509637248 7 46 1.0971542349242185 7 47 1.1258842191967737 7 48 0.957982093235966 7 49 1.4517395223286123 7 50 1.7742406285648897 7 51 2.1892886362692856 7 52 1.2161832954052194 7 53 1.6043667243949784 7 54 1.3306043525069526 7 55 1.1601380484269121 7 56 0.11531104701703576 8 1 -0.2594342963500864 8 2 -0.172407795327004 8 3 -0.23130866476348189 8 4 0.2012027081126675 8 5 0.06448830972426833 8 6 -0.20836057608252645 8 7 -0.2659997627875228 8 8 -0.22586758910178872 8 9 -0.23601591870173166 8 10 0.7874088469060823 8 11 0.8078043010105418 8 12 0.8931107458667782 8 13 1.0826943166279364 8 14 0.4951500219101561 8 15 0.5091220792124176 8 16 0.672566916601653 8 17 0.9380031517145682 8 18 0.8254629006975662 8 19 1.082759796885604 8 20 0.782901272972488 8 21 1.144988325354248 8 22 1.073906599076203 8 23 1.0597204447836646 8 24 1.2089823477514656 8 25 1.1208234268104533 8 26 1.4242504315877795 8 27 0.922775810735935 8 28 0.9940680351079649 8 29 0.4497712428305576 8 30 0.9725273343792082 8 31 0.828927553311793 8 32 1.3427881833369355 8 33 0.9719310415295652 8 34 1.2662657432278692 8 35 1.690721638966025 8 36 1.7074164801862182 8 37 2.035727264429738 8 38 2.35129343856741 8 39 2.121356010857639 8 40 2.3865953124214982 8 41 2.9675754153868787 8 42 3.1320053751231107 8 43 2.7341027330215013 8 44 3.1295912313970113 8 45 2.757427102012617 8 46 2.4363827483462828 8 47 2.5790310723436285 8 48 3.375396936457264 8 49 3.2474083696103655 8 50 4.118025875575942 8 51 4.316065085626384 8 52 4.273977114489359 8 53 4.864957746276325 8 54 4.67141193202421 8 55 4.361401301303069 8 56 3.420792662176071 9 1 0.08729431750181504 9 2 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120 1 -0.034315044740772294 120 2 -0.32389207263909725 120 3 -0.3331009122510977 120 4 -0.4130241246572126 120 5 -0.5845646240968776 120 6 -0.15158291665586177 120 7 -0.08936844708335911 120 8 -0.2706969319443786 120 9 -0.3407257783784772 120 10 -0.16762287633756046 120 11 -0.659013649251122 120 12 -1.2922734064047887 120 13 -0.8374186829552599 120 14 -0.9212349176535923 120 15 -0.7162750402244313 120 16 -0.7073247149456066 120 17 -0.642138560877477 120 18 -0.7873282816300389 120 19 -0.9396085632311042 120 20 -0.6753490859401268 120 21 -0.38805479047032776 120 22 -0.6673058308779503 120 23 -0.3609251348023079 120 24 -0.6627816498403476 120 25 -0.7717806578466124 120 26 0.10961142820705305 120 27 -0.2069421534191136 120 28 -0.0737317148109895 120 29 -0.17260637954706226 120 30 -0.04317219787661486 120 31 -0.09711329325885742 120 32 0.1392499996662977 120 33 0.3233412207543154 120 34 0.4093676744092747 120 35 -0.08855868336065953 120 36 0.794666978032744 120 37 0.7340554263340593 120 38 0.9528309738797773 120 39 1.0280387321098985 120 40 1.3889108776509715 120 41 1.6754900173412146 120 42 1.3112301238842825 120 43 1.309798560372954 120 44 1.57570802850719 120 45 2.0981856811817927 120 46 1.8488312270199287 120 47 2.0901226297927984 120 48 1.8579607302793342 120 49 2.468915339461706 120 50 2.5847512032284214 120 51 2.9953129900398494 120 52 3.1748246113633485 120 53 3.1325444641941687 120 54 2.7759365464825976 120 55 3.6475125410566918 120 56 1.6951871418685638 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 413.0 5 24.0 6 62.0 7 88.0 8 296.0 9 318.0 10 202.0 11 292.0 12 290.0 13 353.0 14 507.0 15 604.0 16 706.0 17 928.0 18 1219.0 19 1338.0 20 1450.0 21 1810.0 22 1805.0 23 1840.0 24 2421.0 25 2722.0 26 2809.0 27 2963.0 28 3498.0 29 3907.0 30 4428.0 31 5298.0 32 6408.0 33 7845.0 34 10002.0 35 12441.0 36 16216.0 37 23636.0 38 25433.0 39 20975.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 98.78403112107137 0.8106459192857617 0.315318308395803 0.09000465124707786 8 97.4945764166279 1.7699917211039335 0.3589535958810981 0.3764782663870777 9 91.10101662971844 5.478202564830532 1.9939956628631146 1.4267851425879057 10 43.53688076497913 38.153515315892164 9.520559116142243 8.789044802986464 11 27.08777567699807 38.636157707478844 19.313548418273964 14.96251819724912 12 38.24774837357367 23.592695729913558 19.48751714014751 18.67203875636526 13 22.102484490809257 23.465843536880765 26.80386838782944 27.627803584480542 14 26.754335626740446 31.53666330407679 23.173479434843276 18.535521634339496 15 30.63601273354395 26.77547765891257 20.245610008033974 22.342899599509504 16 26.312165125311843 31.71486043238476 24.2861543851595 17.686820057143894 17 34.12323992582167 25.256271632829346 21.83065836288184 18.789830078467144 18 26.951862613034365 27.67431605525923 24.261388004615004 21.112433327091402 19 29.24728324886588 27.825934628836524 23.41570671772971 19.511075404567887 20 21.323249590750663 31.842316683479616 23.96298332195691 22.871450403812815 21 22.016104187934545 29.12405540420545 30.0555129359034 18.804327471956604 22 26.030674068391452 29.02680205621364 23.1360278349955 21.806496040399402 23 25.046663485294207 33.862286843011354 22.049327381347894 19.04172229034655 24 27.263281259439147 29.877245762199912 22.038976886923535 20.82049609143741 25 26.73498241755586 27.11382064698561 22.645100479740915 23.506096455717618 26 28.42006008111555 25.12406085479591 20.112062764819303 26.343816299269236 27 30.163815511092306 26.203227951399384 19.691108021519675 23.941848515988635 28 29.989175983987714 31.649060003749103 20.03229063994727 18.329473372315917 29 31.47233918977784 30.175209331590917 19.805430521271962 18.547020957359276 30 24.115549258593237 34.3874690449809 23.66870712464958 17.828274571776287 31 24.381201613782576 37.245423375616376 22.031403336604512 16.341971673996536 32 24.347704926970817 35.14124225333342 24.31438593090246 16.196666888793306 33 22.553261106667634 35.45832424440728 23.53183547831988 18.4565791706052 34 21.124848484848485 37.64848484848485 25.797575757575757 15.42909090909091 35 20.18867009409254 39.309705112038024 25.865142108836935 14.636482685032496 36 21.224086271940998 38.73078835959581 23.11826000460958 16.92686536385361 37 20.67946913819876 36.95227581521739 26.68502523291926 15.683229813664596 38 22.900800417503593 36.18809507916183 25.00166880070879 15.909435702625782 39 20.275512867993033 35.04380399609012 25.854982363048006 18.825700772868842 40 20.100624650608854 35.16333276620568 27.635320710691975 17.100721872493498 41 19.24867506199251 35.08958525793747 27.97807166820635 17.683668011863666 42 20.248863277165853 34.66275196343035 26.334281615483746 18.754103143920055 43 19.04113254360147 34.04076767775048 28.50879326700883 18.40930651163922 44 19.120160059353317 33.8228765682107 26.763723934103222 20.293239438332755 45 19.485875568748632 34.227718435971674 27.64544149492688 18.64096450035281 46 19.56843200613396 34.830920520169656 26.279886326986386 19.320761146709994 47 19.685264663805434 36.54217270873284 26.101117097373145 17.671445530088576 48 20.22572510993897 36.453448002826136 26.424943051004373 16.895883836230524 49 20.075990525540245 36.6173255962638 25.81972952731214 17.486954350883817 50 20.205078838983255 35.889406087783 26.221577754490287 17.68393731874345 51 18.957441235769583 37.984930530598824 26.01341268052603 17.044215553105566 52 19.267815692796958 36.0810045144664 27.56017765430519 17.091002138431453 53 19.147574945162077 35.59468681452596 27.954545454545453 17.303192785766512 54 19.41098463918665 37.223879863950586 25.274738767942438 18.09039672892032 55 19.023468454739408 37.47333130143249 25.12343797622676 18.37976226760134 56 20.97346319343711 36.092860538537494 24.21102673184051 18.722649536184893 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 59.5 5 119.0 6 162.5 7 206.0 8 840.5 9 1475.0 10 3363.0 11 5251.0 12 3211.0 13 838.5 14 506.0 15 557.5 16 609.0 17 811.0 18 1013.0 19 1238.5 20 1464.0 21 1539.0 22 1764.5 23 1915.0 24 2288.5 25 2662.0 26 3183.0 27 3704.0 28 4303.5 29 5843.5 30 6784.0 31 7943.0 32 9102.0 33 8332.0 34 7562.0 35 6706.0 36 5850.0 37 4713.0 38 3411.5 39 3247.0 40 3382.0 41 3517.0 42 3426.5 43 3336.0 44 3527.5 45 3719.0 46 5183.5 47 5639.5 48 4631.0 49 4566.0 50 4501.0 51 4921.0 52 5341.0 53 5762.5 54 6519.0 55 6854.0 56 7163.5 57 7473.0 58 7864.0 59 8255.0 60 8774.5 61 9294.0 62 8995.0 63 7602.0 64 6508.0 65 5637.5 66 4767.0 67 4315.5 68 3864.0 69 3448.5 70 3033.0 71 2645.0 72 1872.0 73 1487.0 74 1269.0 75 1051.0 76 801.0 77 551.0 78 461.0 79 306.5 80 242.0 81 185.0 82 128.0 83 94.0 84 60.0 85 43.5 86 27.0 87 19.0 88 9.5 89 8.0 90 4.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.03986783209602107 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.007852754806792029 25 0.02476638054449794 26 0.06282203845433623 27 0.07067479326112826 28 0.105106102798601 29 0.15645103807377966 30 0.234374528079639 31 0.28330323110657396 32 0.2875316375410004 33 0.30867366971313287 34 0.33041975994732614 35 0.36424701142273797 36 0.40653107576700276 37 0.412571656387612 38 0.45787601104218134 39 0.5049925398829336 40 0.5889566105094022 41 0.6107027007435955 42 0.6276163264813014 43 0.6662760424532006 44 0.6692963327635052 45 0.695270829432125 46 0.7345346034660851 47 0.7744024355621062 48 0.824539254713163 49 0.7949404096721777 50 0.8547421578162092 51 0.8299757772717113 52 0.8505137513817828 53 0.8619908545609404 54 1.6098147353923657 55 0.9042749189052051 56 0.889777525415743 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 165547.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.2675433562674 #Duplication Level Percentage of deduplicated Percentage of total 1 75.12332268370608 35.508949120189435 2 11.922044728434505 11.270515321932745 3 4.819169329073483 6.833708856095249 4 2.4472843450479234 4.627084755386688 5 1.4300319488817892 3.379704857230877 6 0.9380191693290735 2.660271705316315 7 0.6440894568690095 2.131116842950944 8 0.45239616613418526 1.7106924317565404 9 0.32971246006389776 1.4026228201054685 >10 1.734185303514377 14.800630636616791 >50 0.1111821086261981 3.528907198559925 >100 0.03578274760383386 3.090965103565755 >500 0.005111821086261981 1.6804895286534942 >1k 0.0076677316293929714 7.374340821639776 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4411 2.6645001117507414 No Hit TAGTCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 2375 1.4346378973946976 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 1720 1.0389798667447916 No Hit TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1539 0.929645357511764 No Hit TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1143 0.6904383649356376 No Hit TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1020 0.6161392233021438 No Hit TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 973 0.5877484943852803 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 617 0.3727038242915909 No Hit TAGTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 607 0.3666632436709817 No Hit TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 585 0.3533739663056413 No Hit TAGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 410 0.24766380544497937 No Hit TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 370 0.22350148296254235 No Hit TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 339 0.20477568303865368 No Hit TAGTCGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 315 0.1902782895491915 No Hit TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 291 0.17578089605972927 No Hit TAGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 230 0.13893335427401282 No Hit TAGTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 211 0.12745625109485526 No Hit TAGTCGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 200 0.12081161241218506 No Hit TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 186 0.11235479954333209 No Hit TAGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAAAAAAAAAAAAAAAAAAA 186 0.11235479954333209 No Hit TAGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC 174 0.105106102798601 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 168 0.10148175442623544 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.002416232248243701 0.0 0.0 0.0 0.0 8 0.002416232248243701 0.0 0.0 0.0 0.0 9 0.004228406434426477 0.0 0.0 0.0 0.0 10 0.01932985798594961 0.0 0.0 0.0 0.0 11 0.03684754178571644 0.0 0.0 0.0 0.0 12 0.04349218046838662 0.0 0.0 0.0 0.0 13 0.05074087721311772 0.0 0.0 0.0 0.0 14 0.0767153738817375 0.0 0.0 0.0 0.0 15 0.09000465124707786 0.0 0.0 0.0 0.0 16 0.10087769636417453 0.0 0.0 0.0 0.0 17 0.11597914791569766 0.0 0.0 0.0 0.0 18 0.12504001884661153 0.0 0.0 0.0 0.0 19 0.14436987683256114 0.0 0.0 0.0 0.0 20 0.16974031543912002 0.0 0.0 0.0 0.0 21 0.18363365086652128 0.0 0.0 0.0 0.0 22 0.2029635088524709 0.0 0.0 0.0 0.0 23 0.23437452807963902 0.0 0.0 0.0 0.0 24 0.26155714087238063 0.0 0.0 0.0 0.0 25 0.28994786978924414 0.0 0.0 0.0 0.0 26 0.3129020761475593 0.0 0.0 0.0 0.0 27 0.3364603405679354 0.0 0.0 0.0 0.0 28 0.3678713597951035 0.0 0.0 0.0 0.0 29 0.38357686940868757 0.0 0.0 0.0 0.0 30 0.3998864370843325 0.0 0.0 0.0 0.0 31 0.4089473080152464 0.0 0.0 0.0 0.0 32 0.4180081789461603 0.0 0.0 0.0 0.0 33 0.42706904987707417 0.0 0.0 0.0 0.0 34 0.4385461530562318 0.0 0.0 0.0 0.0 35 0.46693688197309524 0.0 0.0 0.0 0.0 36 0.477205869028131 0.0 0.0 0.0 0.0 37 0.4868707980211058 0.0 0.0 0.0 0.0 38 0.49713978507614154 0.0 0.0 0.0 0.0 39 0.5074087721311773 0.0 0.0 0.0 0.0 40 0.5225102236827004 0.0 0.0 0.0 0.0 41 0.5454644300410155 0.0 0.0 0.0 0.0 42 0.5708348686475744 0.0 0.0 0.0 0.0 43 0.5956012491920724 0.0 0.0 0.0 0.0 44 0.6149311071780219 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGACCC 20 3.9251635E-4 50.624958 49 GATAATC 15 0.008024514 50.609444 48 GCGATAA 15 0.008290388 50.19398 46 TACTATT 15 0.008290388 50.19398 46 ATAGTAA 15 0.008290388 50.19398 45 TGCGTTC 15 0.008290388 50.19398 45 TAGTAAT 15 0.008290388 50.19398 46 ATATAAG 15 0.008300359 50.178726 44 TATAGTA 15 0.008300359 50.178726 44 CCGTGAT 20 4.100541E-4 50.178722 44 TGGTTAC 15 0.008320329 50.14824 42 TTGCAAT 15 0.008320329 50.14824 42 ATGCTAG 20 4.1128616E-4 50.148235 42 TCATCTC 20 4.1128616E-4 50.148235 42 AAACTGG 15 0.008360375 50.08738 39 TAGCAGG 20 4.1375912E-4 50.087376 39 TAACGAT 20 4.143792E-4 50.072186 37 TGATGCT 20 4.143792E-4 50.072186 38 GTTTAAC 15 0.008390503 50.041832 36 AATCTCT 15 0.008390503 50.041832 36 >>END_MODULE