FastQCFastQC Report
Fri 17 Jun 2016
SRR1526718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45163
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.33655868742111905No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.21920598720191306No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.19484976640170049No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC840.18599295883798683No Hit
TCGTAAGGGGTTGCTGCCAGAATCTCTGGTGGTTTGGATTTTTTTGGAGAAAATATACAATTTA840.18599295883798683No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC830.18377875694705845No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC660.14613732480127536No Hit
TCGTAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT540.1195669021101344No Hit
TCGTAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG470.1040674888736355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAAGG150.00447132758.6015757
GGGCATG150.00461226658.1447687
AGGGTCA150.00461226658.1447686
GGTTTCG258.410088E-658.14476855
AAGGGTC303.6175152E-758.1447685
AGGGAAA150.00461226658.1447686
GGGATGT303.6175152E-758.1447687
AAGCAAC150.00461226658.14476851
AGGGCAT406.7848305E-1058.1447646
AAGGGTT201.9651784E-458.1447645
CGTTCGC201.9760433E-458.0800947
TAGCAAG406.8394E-1058.0800947
ATCACTT150.00463266758.0800950
CTAGCAA351.576882E-858.08008646
CCTAGCA406.91216E-1058.01555645
GGATGTC150.00465313558.0155538
GGAGCAT150.00465313558.0155538
TCAAGTT150.004673670557.95116444
CCGGACG150.004673670557.95116433
AGCTTAG150.004673670557.95116431
Produced by FastQC (version 0.11.5)