FastQCFastQC Report
Fri 17 Jun 2016
SRR1526717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62300
Sequences flagged as poor quality0
Sequence length64
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3590.5762439807383627No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3350.5377207062600321No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3330.5345104333868379No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2740.43980738362760835No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2600.4173354735152488No Hit
TCGTAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC2250.3611556982343499No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2240.3595505617977528No Hit
TCGTAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2070.33226324237560195No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1940.31139646869983945No Hit
TCGTAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1860.2985553772070626No Hit
TCGTAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1750.2808988764044944No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1590.2552166934189406No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1500.2407704654895666No Hit
TCGTAAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1460.23434991974317815No Hit
TCGTAAGGGAGTTTGAGCTTGGCGAGCAGCTGGACAACGAGCGAGTTGGGATGGCTACCAGCTG1440.23113964686998395No Hit
TCGTAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1340.21508828250401285No Hit
TCGTAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1320.2118780096308186No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.20385232744783308No Hit
TCGTAAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1260.20224719101123598No Hit
TCGTAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1250.20064205457463882No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.19903691813804172No Hit
TCGTAAGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTTCTTCTAGC1220.19582664526484753No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1170.18780096308186195No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.18298555377207062No Hit
TCGTAAGGGGTTGCTGCCAGAATCTCTGGTGGTTTGGATTTTTTTGGAGAAAATATACAATTTA1090.17495987158908508No Hit
TCGTAAGGGGTCTCCTTGAGGGCCCCGGGTCTTCACACTCACGGTCCACACCTCCCCGAGGTCC1070.17174959871589085No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1050.16853932584269662No Hit
TCGTAAGGGGTTGTTTTCTGCGATCCTTAGCTCACTAGAAAGTTACTTCTCCCCCACGCTCTTA1020.1637239165329053No Hit
TCGTAAGGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGC1010.1621187800963082No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1010.1621187800963082No Hit
TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCAGTGCCAG1010.1621187800963082No Hit
TCGTAAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1000.16051364365971107No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG980.15730337078651685No Hit
TCGTAAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC980.15730337078651685No Hit
TCGTAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA940.15088282504012843No Hit
TCGTAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG940.15088282504012843No Hit
TCGTAAGGGGCTCTCACAGTAAGTACCAGTTCTGCTTTCAGCCTGCTCCAGACCTGCTCCTGCA910.14606741573033707No Hit
TCGTAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT900.14446227929373998No Hit
TCGTAAGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCC900.14446227929373998No Hit
TCGTAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC870.13964686998394865No Hit
TCGTAAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGAC850.13643659711075443No Hit
TCGTAAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGGCAGGCAC830.1332263242375602No Hit
TCGTAAGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC810.13001605136436598No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC800.12841091492776885No Hit
TCGTAAGGGGTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCCC780.12520064205457465No Hit
TCGTAAGGGGGCTCTACTGGGCTCTCCTTGAGGGCCCCGGGTCTTCACACTCACGGTCCACACC770.12359550561797752No Hit
TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA760.12199036918138043No Hit
TCGTAAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCCTTCGTTGC750.1203852327447833No Hit
TCGTAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT690.11075441412520064No Hit
TCGTAAGGGGCAGAAACTCGGGATAGTGAATAAGACGTAGCACTAATTAGACTGATGATCATTG690.11075441412520064No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT650.1043338683788122No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC640.10272873194221509No Hit
TCGTAAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGAGAC640.10272873194221509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGA150.00457318158.29611658
TCATTCA201.9614912E-458.2019456
CCGAGGT201.9614912E-458.2019456
GTCCGTC201.9614912E-458.2019456
TTCATTC201.9614912E-458.2019455
TCACGCG150.004602582758.20193557
CTCACGC150.004602582758.20193556
TTGAACG150.004602582758.20193557
ACGCTCT258.398698E-658.20193556
CATACAG150.004602582758.20193555
CTACCAG258.398698E-658.20193555
AAAGTTA258.439067E-658.15496439
TGGGATG258.439067E-658.15496447
CCTTCGA150.00461733658.1549654
ACCGGAG150.00461733658.1549654
CGTGCGT201.969348E-458.1549647
ACTTGAT150.00461733658.1549640
GCAAAGT150.00461733658.1549649
TAAACAC150.00461733658.1549639
TGAACGC150.00461733658.1549639
Produced by FastQC (version 0.11.5)