FastQCFastQC Report
Fri 17 Jun 2016
SRR1526716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62914
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3590.5706202117175828No Hit
TCGTAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3160.5022729440188193No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3060.4863782306005023No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3010.47843087389134376No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2680.42597831961089744No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2210.35127316654480717No Hit
TCGTAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC2120.33696792446832186No Hit
TCGTAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1910.303589026289856No Hit
TCGTAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1710.27179959945322185No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1660.2638522427440633No Hit
TCGTAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1480.2352417585910926No Hit
TCGTAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1450.2304733445655975No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1420.22570493054010235No Hit
TCGTAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1370.21775757383094385No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1370.21775757383094385No Hit
TCGTAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1310.20822074577995356No Hit
TCGTAAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1290.20504180309629016No Hit
TCGTAAGGGAGTTTGAGCTTGGCGAGCAGCTGGACAACGAGCGAGTTGGGATGGCTACCAGCTG1270.20186286041262674No Hit
TCGTAAGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTTCTTCTAGC1160.18437867565247798No Hit
TCGTAAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1150.1827892043106463No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1120.17802079028515116No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.17325237625965606No Hit
TCGTAAGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC1080.17166290491782435No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.1653050195504975No Hit
TCGTAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1040.1653050195504975No Hit
TCGTAAGGGGTCTCCTTGAGGGCCCCGGGTCTTCACACTCACGGTCCACACCTCCCCGAGGTCC1030.1637155482086658No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.16212607686683408No Hit
TCGTAAGGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGC1020.16212607686683408No Hit
TCGTAAGGGGTTGTTTTCTGCGATCCTTAGCTCACTAGAAAGTTACTTCTCCCCCACGCTCTTA970.15417872015767556No Hit
TCGTAAGGGGTTGCTGCCAGAATCTCTGGTGGTTTGGATTTTTTTGGAGAAAATATACAATTTA960.15258924881584385No Hit
TCGTAAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC950.15099977747401214No Hit
TCGTAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA950.15099977747401214No Hit
TCGTAAGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCC940.14941030613218043No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC920.146231363448517No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT900.1430524207648536No Hit
TCGTAAGGGGCTCTCACAGTAAGTACCAGTTCTGCTTTCAGCCTGCTCCAGACCTGCTCCTGCA900.1430524207648536No Hit
TCGTAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG880.1398734780811902No Hit
TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCAGTGCCAG880.1398734780811902No Hit
TCGTAAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC850.13510506405569506No Hit
TCGTAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT830.13192612137203166No Hit
TCGTAAGGGGCAGAAACTCGGGATAGTGAATAAGACGTAGCACTAATTAGACTGATGATCATTG790.12556823600470482No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC790.12556823600470482No Hit
TCGTAAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTGGCAGGCAC750.119210350637378No Hit
TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA740.1176208792955463No Hit
TCGTAAGGGGTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCCC740.1176208792955463No Hit
TCGTAAGGGGAGCTGCAGGCCTAGCGGGGGACCAGGTTCGGGTGACCCTAGGCTTCCCAGACCG710.11285246527005117No Hit
TCGTAAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGAGAC700.11126299392821948No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG700.11126299392821948No Hit
TCGTAAGGGGCTTCTCTTAGGCGGAGCGGCTTTCTGCATAGTCCCCTCCTTTTCTTCCTTTCCC680.10808405124455606No Hit
TCGTAAGGGGGCTCTACTGGGCTCTCCTTGAGGGCCCCGGGTCTTCACACTCACGGTCCACACC670.10649457990272436No Hit
TCGTAAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGAC670.10649457990272436No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC660.10490510856089265No Hit
TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG650.10331563721906094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTC150.004523685758.45696345
CAAGTTT150.004523685758.45696345
GTTGGGA258.1842445E-658.4569645
CGGCCAA406.530172E-1058.45695545
CGCATCT150.004538128658.4099742
GACAAAC150.004538128658.4099726
TTTCAAG150.004538128658.4099742
GCCGCAT150.004538128658.4099740
TCTCGTT150.004538128658.4099743
GCACTAA150.004538128658.4099740
TAGTCCC150.004538128658.4099739
CGAGTTG258.2234255E-658.40996642
TAGTTGT258.2234255E-658.40996639
AGTTGTC258.2234255E-658.40996640
TAGCGGG150.004552605658.36305222
ACTACCC150.004552605658.36305222
AGTACCA150.004552605658.36305222
GCAAATG150.004567117458.3162120
TAAGTAC150.004567117458.3162120
TTAAGGA150.004567117458.3162120
Produced by FastQC (version 0.11.5)