FastQCFastQC Report
Fri 17 Jun 2016
SRR1526715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58237
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3770.6473547744561018No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3710.6370520459501692No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3200.5494788536497416No Hit
TCGTAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT2990.5134193038789773No Hit
TCGTAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT2220.3812009547195082No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2040.3502927692017102No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2030.3485756477840548No Hit
TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1920.3296873121898449No Hit
TCGTAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC1900.3262530693545341No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1670.2867592767484589No Hit
TCGTAAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT1490.2558510912306609No Hit
TCGTAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1470.25241684839535006No Hit
TCGTAAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1380.23696275563645106No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.23696275563645106No Hit
TCGTAAGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT1350.23181139138348472No Hit
TCGTAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1350.23181139138348472No Hit
TCGTAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1320.22666002713051842No Hit
TCGTAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG1300.2232257842952075No Hit
TCGTAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1280.21979154145989663No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1260.21635729862458575No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC1240.21292305578927487No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.209488812953964No Hit
TCGTAAGGGGTTTTCATTAATATATTTATTTATTCATTTTACATCCAAATATCAGC1180.2026203272833422No Hit
TCGTAAGGGAGTTTGAGCTTGGCGAGCAGCTGGACAACGAGCGAGTTGGGATGGCT1160.19918608444803132No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1150.19746896303037587No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1090.18716623452444323No Hit
TCGTAAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC1040.178580627436166No Hit
TCGTAAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1030.17686350601851059No Hit
TCGTAAGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT1000.17171214176554425No Hit
TCGTAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA980.16827789893023337No Hit
TCGTAAGGGGTCTCCTTGAGGGCCCCGGGTCTTCACACTCACGGTCCACACCTCCC940.16140941325961158No Hit
TCGTAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG940.16140941325961158No Hit
TCGTAAGGGGTTGCTGCCAGAATCTCTGGTGGTTTGGATTTTTTTGGAGAAAATAT930.15969229184195616No Hit
TCGTAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG910.15625804900664525No Hit
TCGTAAGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCT850.1459553205007126No Hit
TCGTAAGGGGTTGTTTTCTGCGATCCTTAGCTCACTAGAAAGTTACTTCTCCCCCA850.1459553205007126No Hit
TCGTAAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA850.1459553205007126No Hit
TCGTAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG830.14252107766540173No Hit
TCGTAAGGGGTCTTGATATTCTAAGATCACAGTGGTCTCCTGCTTTAACTATTTCT830.14252107766540173No Hit
TCGTAAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT810.13908683483009082No Hit
TCGTAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA810.13908683483009082No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG800.1373697134124354No Hit
TCGTAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT790.13565259199477994No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA760.1305012277418136No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC760.1305012277418136No Hit
TCGTAAGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCC760.1305012277418136No Hit
TCGTAAGGGGGGTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCC740.12706698490650273No Hit
TCGTAAGGGGGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCGCC730.1253498634888473No Hit
TCGTAAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG730.1253498634888473No Hit
TCGTAAGGGGCAGAAACTCGGGATAGTGAATAAGACGTAGCACTAATTAGACTGAT720.12363274207119186No Hit
TCGTAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT680.11676425640057009No Hit
TCGTAAGGGGCTCTCACAGTAAGTACCAGTTCTGCTTTCAGCCTGCTCCAGACCTG670.11504713498291465No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT660.11333001356525921No Hit
TCGTAAGGGGTTCCTTTGCTTCATAAAGAGAGGGACAAGAGCTGGTGCTGTGGACA650.11161289214760375No Hit
TCGTAAGGGGAGGCAGATGCTTTAGCTCTGCAGTGGAGTCCCGCTGTATCTGACCA640.10989577072994831No Hit
TCGTAAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTA630.10817864931229287No Hit
TCGTAAGGGGCTTCTCTTAGGCGGAGCGGCTTTCTGCATAGTCCCCTCCTTTTCTT610.104744406476982No Hit
TCGTAAGGGGAGGCTCTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGG590.1013101636416711No Hit
TCGTAAGGGGAATATGACTGTCTTCCCATCTCTCTGCAAGAAGGCAGAAGCTGCTA590.1013101636416711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCAA203.8762027E-450.6972949
ACACCTC251.9002062E-550.6972948
TAATTCA203.8762027E-450.6972950
ACCTCCC251.9002062E-550.6972950
TTCGCGC150.00794337450.69728549
TGATACA150.00794337450.69728550
GTTCGCG150.00794337450.69728548
CATTGAT150.00819381850.30121647
AAAGATT354.933645E-850.30121647
TGTTAGT150.008222003550.25758745
CAAAGAT354.967478E-850.25758746
CGAGTAT150.008222003550.25758746
ACGGCGG150.008222003550.25758746
AACACCT150.008222003550.25758745
TTGTTAG150.00825026150.2140444
TCGACAG150.00825026150.2140443
TTCAAAC150.00825026150.2140444
ATTGTTA150.00825026150.2140443
CTGCATT150.00825026150.2140444
ATCCAGG150.0082785950.17056340
Produced by FastQC (version 0.11.5)