FastQCFastQC Report
Fri 17 Jun 2016
SRR1526714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376920
Sequences flagged as poor quality0
Sequence length64
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107132.8422476918178923No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43881.164172768757296No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39631.051416746259153No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26710.708638437864799No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23550.6248010187838269No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16030.4252891860341717No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11970.31757402101241644No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9500.2520428738193781No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9220.24461424174891225No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8060.21383848031412503No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7570.20083837419080974No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7350.1950015918497294No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6970.18491987689695427No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAGCAGTGGTATCAA6050.1605115143797092No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5410.14353178393293006No Hit
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5170.13716438501538789No Hit
AATAAAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5040.13371537726838587No Hit
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4970.1318582192507694No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA4460.11832749655099224No Hit
AATAAAGGGGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4440.1177968799745304No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4360.11567441366868302No Hit
AATAAAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4340.11514379709222117No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.1079804733099862No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3960.10506208213944604No Hit
AATAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3820.1013477661042131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAGTA201.9109901E-458.58746357
CGTCCGA150.00470870757.92542330
CTAGATA150.004741010357.8254819
ACTAGAT150.004748488357.80246718
ATGTCGA404.696085E-850.74545738
TGTCGAA404.696085E-850.74545739
AAGGGAT10500.050.369445
AGGGTAT5450.050.1206136
AGGGGCT6350.049.88136
AAGGGTC4700.049.4626245
AGGGTAC2650.049.3459666
AGGGATT1950.049.177266
AAAGGGT30650.049.016814
AAGGGGT40250.048.8773655
AGGGGTG25450.048.7556656
AGGGAAG39400.048.3092276
AGGGTCA3450.048.0109986
AAGCAGT21450.047.8318810
AAGGGTG12650.047.321855
AAGGGTA11900.047.1297725
Produced by FastQC (version 0.11.5)