FastQCFastQC Report
Fri 17 Jun 2016
SRR1526713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495845
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150793.0410713025239744No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64921.3092801177787412No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55071.1106293297300567No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37880.7639484112978854No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37460.7554780223658603No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19920.40173844649033463No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19620.39568816868174533No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT17970.3624116407345037No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13570.2736742328751928No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12450.2510865290564592No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11540.23273401970373805No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9870.19905413990259055No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9330.18816363984712964No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA8900.17949157498815155No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA8450.17041615827526746No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA8330.16799604715183172No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7970.16073571378152446No Hit
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7210.14540834333309804No Hit
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7110.14339158406356825No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6540.13189605622724843No Hit
AATAAAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6380.12866924139600078No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5950.11999717653702266No Hit
AATAAAGGGGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5840.1177787413405399No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5710.11515695429015116No Hit
AATAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5680.11455192650929222No Hit
AATAAAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5190.10466980608859623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACA150.004681813558.01202433
GCGTAAC150.004681813558.01202434
AAGCGAT202.0253E-457.9067210
ATATCGG150.00471561457.90671510
CTGCATA150.00471561457.90671512
AGGGTAC4400.051.3626636
AAGGGAT13700.050.334075
AGGGTAT7250.049.954856
AGGGATT3000.049.2554786
AAGGGTG15250.048.8279955
AGGGAAG41000.048.619476
AAGCAGT21750.048.58839810
AAAGGGT40000.048.5311364
GGAAGCA29350.048.4706658
AGGGGTG33550.048.448016
AAGGGAC26000.048.252545
AGGGGCT12000.048.0482376
GAAGCAG23250.047.9442679
AGGGATG5500.047.9384886
AGGGACT4150.047.4751636
Produced by FastQC (version 0.11.5)