FastQCFastQC Report
Fri 17 Jun 2016
SRR1526711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406915
Sequences flagged as poor quality0
Sequence length56
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121632.9890763427251392No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49801.2238428172959954No Hit
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43991.0810611552781295No Hit
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT41231.0132337220303995No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29390.7222638634604279No Hit
AATAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG17780.4369462909944337No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16590.4077018541955937No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15130.3718221250138235No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA13260.32586658147278913No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAA12120.29785090252263985No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9510.23370974282098228No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA7710.18947446026811496No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA7700.18922870869837682No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7350.1806274037575415No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7160.1759581239325166No Hit
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAA6540.16072152660875122No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAA6210.1526117248073922No Hit
AATAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.14769669341262917No Hit
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAA5770.14179865573891354No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA5740.14106140102969908No Hit
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5580.13712937591388866No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAAAAAAAAA5410.13295159922834007No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA5090.1250875489967192No Hit
AATAAAGGGGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT5040.12385879114802847No Hit
AATAAAGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA4650.11427447992824055No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAAAAA4600.11304572207954978No Hit
AATAAAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4490.11034245481243012No Hit
AATAAAGGGGAGTGCAGTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4370.1073934359755723No Hit
AATAAAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4260.10469016870845263No Hit
AATAAAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.10395291399923816No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA4170.10247840458080926No Hit
AATAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4170.10247840458080926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAT150.00828706150.21238345
TCACGCC150.00829923950.19373742
ATTGCGA252.0694442E-550.06358734
CGCGATT301.0512704E-649.9648830
TACTACG150.00850829549.8788322
GGACGGT150.00852485649.8542988
GACAGTA150.0085455949.823679
TCGTTAC150.0085455949.8236717
GATCGTT150.0085455949.8236715
CTATCGA150.0085455949.8236717
ATCGTTA150.0085455949.8236716
ACTAGTT150.0085455949.8236714
ACGTGTC150.0085455949.8236710
TTGCATG150.0085455949.8236717
ATCGGTT150.0085455949.8236710
GACTAGT150.0085455949.8236713
GTCCATG355.426955E-849.82366610
AAGGGAT11550.044.8904465
AGGGGAT4450.044.812856
AAGGGAC20950.044.1430635
Produced by FastQC (version 0.11.5)