FastQCFastQC Report
Fri 17 Jun 2016
SRR1526709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29203
Sequences flagged as poor quality0
Sequence length64
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5341.8285792555559361No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3561.2190528370372906No Hit
ATTCCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2991.0238674108824435No Hit
ATTCCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2360.8081361503955072No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1370.469129883916036No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1170.40064376947573876No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1090.3732493236996199No Hit
ATTCCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.345854877923501No Hit
ATTCCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.29106598637126324No Hit
ATTCCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.2568229291511146No Hit
ATTCCAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGC720.24655001198507004No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.23970140054104028No Hit
ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.21573126048693628No Hit
ATTCCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.16779098037872822No Hit
ATTCCAGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCA460.15751806321268363No Hit
ATTCCAGGGGCTATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAA460.15751806321268363No Hit
ATTCCAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.1506694517686539No Hit
ATTCCAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.14724514604663902No Hit
ATTCCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.1403965346026093No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.13012361743656475No Hit
ATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.12327500599253502No Hit
ATTCCAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG350.11985070027052014No Hit
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.11642639454850529No Hit
ATTCCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA330.11300208882649043No Hit
ATTCCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.10957778310447557No Hit
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC320.10957778310447557Illumina Single End Adapter 2 (96% over 31bp)
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG310.10615347738246071No Hit
ATTCCAGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.10272917166044586No Hit
ATTCCAGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.10272917166044586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGGC351.5154E-858.2554
ATCATGG351.5154E-858.2553
CATGGCT351.5154E-858.2555
CTTGAAC150.00456302258.2558
TGCCGTG258.331386E-658.14974639
CAGTACG351.5363185E-858.14974237
TGATCAT351.5363185E-858.14974251
ACGTAGA351.5363185E-858.14974241
CGTAGAG351.5363185E-858.14974242
AGTACGT351.5363185E-858.14974238
TCAGTAC351.5363185E-858.14974236
TACGTAG351.5363185E-858.14974240
CGTTGCC201.9527347E-458.14974236
GTACGTA351.5363185E-858.14974239
TTGCCGT201.9527347E-458.14974238
GTTTGAT303.5706216E-758.1497448
CCGTGCG150.00459432958.1497441
GAGTTTG303.5706216E-758.1497446
TAGAGTT303.5706216E-758.1497444
AGTTTGA303.5706216E-758.1497447
Produced by FastQC (version 0.11.5)