FastQCFastQC Report
Fri 17 Jun 2016
SRR1526708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28643
Sequences flagged as poor quality0
Sequence length64
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5351.867821108124149No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3251.134657682505324No Hit
ATTCCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2790.9740599797507246No Hit
ATTCCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.8309185490346682No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1130.39451174807108197No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1100.38403798484795587No Hit
ATTCCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.37007296721712113No Hit
ATTCCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.3002478790629473No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.2932653702475299No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG830.28977411583982127No Hit
ATTCCAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGC620.21645777327793875No Hit
ATTCCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.202492755647104No Hit
ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.1710714659777258No Hit
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.16408895716230842No Hit
ATTCCAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.16059770275459972No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.15012393953147365No Hit
ATTCCAGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCA420.14663268512376496No Hit
ATTCCAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.13965017630834758No Hit
ATTCCAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.12917641308522154No Hit
ATTCCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.12219390426980416No Hit
ATTCCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.12219390426980416No Hit
ATTCCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA330.11521139545438676No Hit
ATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.11172014104667807No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG320.11172014104667807No Hit
ATTCCAGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.1047376322312607No Hit
ATTCCAGGGAGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.101246377823552No Hit
ATTCCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.101246377823552No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGTT150.004424411358.70053545
TAGAGTT351.4439138E-858.5960944
TCCTCGT150.004455803458.59608544
CTCCTCG150.004455803458.59608543
CTCTCCT150.004455803458.59608541
ACTCTCC150.004487361358.4920140
GACTCTC150.004487361358.4920139
AGCTCGC150.00451908558.38829857
CTGGCTC150.00451908558.38829856
GCAGCTC150.00451908558.38829855
GCTCGCT150.00451908558.38829858
TCATGGC258.125462E-658.38829854
CCTGGCT150.00451908558.38829855
GTTTGAT351.5063051E-858.28495848
TGATCAT258.211609E-658.28495851
GAGTTTG406.475602E-1058.28495846
TTGATCA258.211609E-658.28495850
ATCATGG258.211609E-658.28495853
AGTTTGA406.475602E-1058.28495847
CGTTCGC150.004550975358.28495448
Produced by FastQC (version 0.11.5)