FastQCFastQC Report
Fri 17 Jun 2016
SRR1526707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24215
Sequences flagged as poor quality0
Sequence length56
%GC34

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT6042.4943217014247367No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3691.5238488540161057No Hit
ATTCCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2280.9415651455709271No Hit
ATTCCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.8920090852777205No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1250.5162089613875697No Hit
ATTCCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1140.47078257278546354No Hit
ATTCCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.42122651249225684No Hit
ATTCCAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG820.3386330786702457No Hit
ATTCCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.3014660334503407No Hit
ATTCCAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.26842865992153625No Hit
ATTCCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.23126161470163123No Hit
ATTCCAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG560.23126161470163123No Hit
ATTCCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.22300227131943012No Hit
ATTCCAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.19822424117282678No Hit
ATTCCAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.18996489779062564No Hit
ATTCCAGGGGCTATTCCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAA440.18170555440842454No Hit
ATTCCAGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1651868676440223No Hit
ATTCCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.1569275242618212No Hit
ATTCCAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC370.15279785257072062No Hit
ATTCCAGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAG360.14866818087962008No Hit
ATTCCAGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA330.13627916580631838No Hit
ATTCCAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.13214949411521784No Hit
ATTCCAGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.1280198224241173No Hit
ATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.1280198224241173No Hit
ATTCCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC290.11976047904191617No Hit
ATTCCAGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT290.11976047904191617No Hit
ATTCCAGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.11563080735081561No Hit
ATTCCAGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.11563080735081561No Hit
ATTCCAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC280.11563080735081561No Hit
ATTCCAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.11150113565971506No Hit
ATTCCAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC260.1073714639686145No Hit
ATTCCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1073714639686145No Hit
ATTCCAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.1073714639686145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAG150.007851982550.75368550
AGTCGGA150.007917738550.6470649
TATGCGT251.9978925E-550.12058332
ATGCGTT251.9978925E-550.12058333
GCGTTGC150.00825268150.12058335
TGGGCAA150.00825268150.12058332
CGTTGCC150.00825268150.12058336
GTATGCG252.0227171E-550.01659831
ACGTATG150.00832091550.01659829
AGGGGTC355.1591996E-849.809926
AGGGCTG355.1591996E-849.809926
GGGAGTC355.1591996E-849.809928
CCGTAAA355.1591996E-849.8099220
AGGGGAT301.0323547E-649.8099176
AGGGGAC204.1804265E-449.8099176
GGGTCGA204.1804265E-449.8099178
GTGTCCC150.00845863949.80991716
TGTCCCG150.00845863949.80991717
AGGGTTC150.00845863949.8099176
AGGGCCT150.00845863949.8099176
Produced by FastQC (version 0.11.5)