FastQCFastQC Report
Fri 17 Jun 2016
SRR1526706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24829
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.5034435539087357No Hit
CTGAAAGGGGTCATAGTTAAAGTCAAAGACAGAGGATATGTGCTCCCAAGGGAGCATACTCCAT1230.495388457046196No Hit
CTGAAAGGGACCATAACTGCCCAACTGTCCAATATGTCAATAGAGTTTGCTCTAAGAGCTGAGT1210.4873333601836562No Hit
CTGAAAGGGCTGTAATGGCCTATAGGTGTGCACTTTAGTGTTTTTGAATTATTTTATTGCTGGG890.3584518103830198No Hit
CTGAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.3222038745015909No Hit
CTGAAAGGGTGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTAC680.27387329332635224No Hit
CTGAAAGGGGATCACACCCACAGCATGCTCTCTCCCTATAGGCACGGCCTCCGCCGTGTGGTCC670.26984574489508234No Hit
CTGAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.2577630996012727No Hit
CTGAAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG630.2537355511700028No Hit
CTGAAAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA590.23762535744492325No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA570.22957026058238347No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA560.22554271215111363No Hit
CTGAAAGGGGAACAAAAGCGTTTTCAGCTACTGAGTGTTGTTTTTTGTTTGTTTGTTTTTTCCC550.22151516371984376No Hit
CTGAAAGGGTGGCATTATTGTTGACTATGCTTGCCTTCCTATTCTAGTATGAACTAATGGTGGT530.21346006685730398No Hit
CTGAAAGGGACTCTTACCTGAAAGATTCACAGAAGTGTCAAGTGCTATGCATTGAAACTTGTTT480.19332232470095453No Hit
CTGAAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAG420.16915703411333521No Hit
CTGAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC360.1449917435257159No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT340.13693664666317612No Hit
CTGAAAGGGGTCCCTTGCTCTGTACCGGTTCTCTCTGAGGTGAGGAGAAAAGTGATGCTTTTAC310.12485400136936647No Hit
CTGAAAGGGTACCCAGACCTCTCTACCTTGTGAAACAATTGTCAGCCCTTTGGTGCCTATCCTT290.1167989045068267No Hit
CTGAAAGGGGAGTCGGAAAACCAATGAGGAAGACCTTTGTATCGCAAGCAAGTGCTACGATGCA260.10471625921301703No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT260.10471625921301703No Hit
CTGAAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG250.10068871078174714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCT150.00451812658.36863756
CAACCTA150.00451812658.36863757
AACCTAC150.00451812658.36863758
TTATAGA150.00451812658.36863756
TATAGAA150.00451812658.36863757
GAGCTGA258.110283E-658.36863356
AAGGGCA750.058.131855
AGGGGTC406.5847416E-1058.1318476
AGGGCAG406.5847416E-1058.1318476
AGGGTAC201.9498455E-458.1318476
AAGGGGC2900.058.1318475
AGGGACC406.5847416E-1058.1318476
AGGGGTA351.5270416E-858.1318446
AGGGGAT650.058.1318446
AAGGGTT150.00459165358.1318445
AAGGGTC150.00459165358.1318445
AAGGGCG303.555724E-758.1318445
AAGGGAT2050.058.1318445
AGGGATT600.058.1318446
AGGGATG1050.058.1318446