FastQCFastQC Report
Fri 17 Jun 2016
SRR1526705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34717
Sequences flagged as poor quality0
Sequence length64
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG9902.8516288849843017No Hit
CTGAAAGGGGATCACACCCACAGCATGCTCTCTCCCTATAGGCACGGCCTCCGCCGTGTGGTCC2810.8094017340207968No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2730.7863582682835499No Hit
CTGAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2400.6913039721174065No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2160.6221735749056658No Hit
CTGAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.5789670766483279No Hit
CTGAAAGGGACCATAACTGCCCAACTGTCCAATATGTCAATAGAGTTTGCTCTAAGAGCTGAGT1640.47239104761356104No Hit
CTGAAAGGGGTCATAGTTAAAGTCAAAGACAGAGGATATGTGCTCCCAAGGGAGCATACTCCAT1530.4407062822248466No Hit
CTGAAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1280.3686954517959501No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1270.3658150185787942No Hit
CTGAAAGGGGGGAGAAGGTGGCTTCTGTGTTTGACTTCAATGCTTGCCAAGAGCAAGACGCCTG1240.35717371892732663No Hit
CTGAAAGGGGACACCAAACCTCAGCTCAAACCCTCCAGAAAGTGAATCTACATCCCTTAGGGCC1220.3514128524930149No Hit
CTGAAAGGGTGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTAC1190.34277155284154737No Hit
CTGAAAGGGCTGTAATGGCCTATAGGTGTGCACTTTAGTGTTTTTGAATTATTTTATTGCTGGG990.2851628884984302No Hit
CTGAAAGGGTACCCAGACCTCTCTACCTTGTGAAACAATTGTCAGCCCTTTGGTGCCTATCCTT960.27652158884696254No Hit
CTGAAAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA950.27364115562980673No Hit
CTGAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA950.27364115562980673No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA910.2621194227611833No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA910.2621194227611833No Hit
CTGAAAGGGTGGCATTATTGTTGACTATGCTTGCCTTCCTATTCTAGTATGAACTAATGGTGGT840.24195639024109225No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA820.23619552380678052No Hit
CTGAAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT810.23331509058962469No Hit
CTGAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC750.21603249128668953No Hit
CTGAAAGGGGACCGCAAAGCAGGAGCTGGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGC730.2102716248523778No Hit
CTGAAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC730.2102716248523778No Hit
CTGAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.20739119163522193No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG710.2045107584180661No Hit
CTGAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.2045107584180661No Hit
CTGAAAGGGGAGTCGGAAAACCAATGAGGAAGACCTTTGTATCGCAAGCAAGTGCTACGATGCA710.2045107584180661No Hit
CTGAAAGGGAGGAGCTGGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA690.19874989198375437No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT680.19586945876659848No Hit
CTGAAAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTGCCTGGCTC650.1872281591151309No Hit
CTGAAAGGGGAACAAAAGCGTTTTCAGCTACTGAGTGTTGTTTTTTGTTTGTTTGTTTTTTCCC620.17858685946366332No Hit
CTGAAAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGAGAC620.17858685946366332No Hit
CTGAAAGGGGGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCGCCTAGCTCTA620.17858685946366332No Hit
CTGAAAGGGGGCTTTTCTTCTTTCCATGCAACTCTCCATCCCATTGTCTCCTCCAGAGAACGCT590.16994555981219575No Hit
CTGAAAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC590.16994555981219575No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC580.1670651265950399No Hit
CTGAAAGGGGTCCCTTGCTCTGTACCGGTTCTCTCTGAGGTGAGGAGAAAAGTGATGCTTTTAC570.16418469337788402No Hit
CTGAAAGGGACTCTTACCTGAAAGATTCACAGAAGTGTCAAGTGCTATGCATTGAAACTTGTTT540.15554339372641646No Hit
CTGAAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAG520.14978252729210473No Hit
CTGAAAGGGGTGTTGTTTTTTAAAAATGAGCATCTTCTGTATGTATCCCACAGTAAGTTCACAT490.14114122764063713No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG490.14114122764063713No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA440.12673906155485784No Hit
CTGAAAGGGGGTCTTCTTCCTCTCATTGAAGAACAGGCCTGGCAAAGATGCACTCTGGCTTCAG420.12097819512054613No Hit
CTGAAAGGGGGCCAGTCAGCTATTGTAGAGAGCTGAACTCGTATTCCATTCCTTTTTTGGGATC410.11809776190339026No Hit
CTGAAAGGGGGACCATGGCCCTTTCTGTGGAGACGGAGTCGCATATCTACCGAGCTCTGCGCAC410.11809776190339026No Hit
CTGAAAGGGGTCCAGGTGCCGGCCTTGTCTGCCATGGACGACTATGCGGTTTTGTCCGATACTG410.11809776190339026No Hit
CTGAAAGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG410.11809776190339026No Hit
CTGAAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAGAAGGT400.11521732868623441No Hit
CTGAAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT380.10945646225192268No Hit
CTGAAAGGGGGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTACCATGAGTTCCGGTG380.10945646225192268No Hit
CTGAAAGGGAGTCTAGACTTTGTGTCTGAGCCCAGCCTGGATCTCCCAGACCACGGGCCGGGTG370.10657602903476682No Hit
CTGAAAGGGAGACAAGGCAGTGAAGGCTGCTCGTGCAGCCTTCCAGCTGGGCTCGCCCTGGCGC370.10657602903476682No Hit
CTGAAAGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC360.10369559581761097No Hit
CTGAAAGGGGGGTTCTCGGGAGAATGAAGTTCGTGTACAAAGAGGAGCATCCGTTCGAGAAGCG360.10369559581761097No Hit
CTGAAAGGGGCCTGGACTTATGTGAAAGACCCTTTGGCACACAACTTAGACTTTGTGTTGTTAT360.10369559581761097No Hit
CTGAAAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTTCATTCAGC360.10369559581761097No Hit
CTGAAAGGGATATTTGAGTCTTTCTCCAAGTTGCGTCGAACAGCCAAGACCAAGGGGCATGGTT350.1008151626004551No Hit
CTGAAAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAAGTCGGGCGG350.1008151626004551No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAACG201.9474236E-458.21304356
GCTAGCT150.004581873358.21304356
AATCAAA150.004581873358.21304358
GCTGGAT150.004581873358.21304356
CTGGATT150.004581873358.21304357
AATGGTG150.004581873358.21304356
TGGATTC150.004581873358.21304358
CTCGGAA150.004581873358.21304355
GTGGTCC406.6393113E-1058.21304358
CATACTC201.9474236E-458.21304355
GCATACT201.9474236E-458.21304354
AAGTTCA150.004581873358.21304355
TATATAA150.004608276358.128852
GGCGAGC150.004608276358.128851
TTATATA150.004608276358.128851
TCTAAGA406.712071E-1058.128851
CGAGCTG150.004608276358.128853
ATATAAA150.004608276358.128853
CCTATAG406.8030204E-1058.044835
GTCAATA406.8030204E-1058.044836
Produced by FastQC (version 0.11.5)