FastQCFastQC Report
Fri 17 Jun 2016
SRR1526704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34792
Sequences flagged as poor quality0
Sequence length64
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG9382.6960220740400094No Hit
CTGAAAGGGGATCACACCCACAGCATGCTCTCTCCCTATAGGCACGGCCTCCGCCGTGTGGTCC3020.8680156357783398No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2660.7645435732352265No Hit
CTGAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.5863416877443091No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1910.548976776270407No Hit
CTGAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1890.543228328351345No Hit
CTGAAAGGGACCATAACTGCCCAACTGTCCAATATGTCAATAGAGTTTGCTCTAAGAGCTGAGT1710.4914922970797884No Hit
CTGAAAGGGGTCATAGTTAAAGTCAAAGACAGAGGATATGTGCTCCCAAGGGAGCATACTCCAT1560.4483789376868246No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1330.38227178661761324No Hit
CTGAAAGGGGGGAGAAGGTGGCTTCTGTGTTTGACTTCAATGCTTGCCAAGAGCAAGACGCCTG1220.35065532306277303No Hit
CTGAAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1190.3420326511841803No Hit
CTGAAAGGGTGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTAC1100.3161646355484019No Hit
CTGAAAGGGGACACCAAACCTCAGCTCAAACCCTCCAGAAAGTGAATCTACATCCCTTAGGGCC1030.29604506783168544No Hit
CTGAAAGGGTACCCAGACCTCTCTACCTTGTGAAACAATTGTCAGCCCTTTGGTGCCTATCCTT930.2673028282363762No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA890.25580593239825244No Hit
CTGAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA890.25580593239825244No Hit
CTGAAAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA830.2385605886410669No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT820.23568636468153598No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA810.23281214072200504No Hit
CTGAAAGGGGAACAAAAGCGTTTTCAGCTACTGAGTGTTGTTTTTTGTTTGTTTGTTTTTTCCC800.2299379167624741No Hit
CTGAAAGGGCTGTAATGGCCTATAGGTGTGCACTTTAGTGTTTTTGAATTATTTTATTGCTGGG790.2270636928029432No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG780.2241894688434123No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA770.22131524488388138No Hit
CTGAAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT740.21269257300528857No Hit
CTGAAAGGGTGGCATTATTGTTGACTATGCTTGCCTTCCTATTCTAGTATGAACTAATGGTGGT730.20981834904575764No Hit
CTGAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.19832145320763392No Hit
CTGAAAGGGGAGTCGGAAAACCAATGAGGAAGACCTTTGTATCGCAAGCAAGTGCTACGATGCA670.19257300528857207No Hit
CTGAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.18969878132904114No Hit
CTGAAAGGGGTCCCTTGCTCTGTACCGGTTCTCTCTGAGGTGAGGAGAAAAGTGATGCTTTTAC650.18682455736951023No Hit
CTGAAAGGGGACCGCAAAGCAGGAGCTGGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGC610.17532766153138651No Hit
CTGAAAGGGAGGAGCTGGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA590.16957921361232467No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC580.16670498965279376No Hit
CTGAAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC580.16670498965279376No Hit
CTGAAAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGAGAC530.1523338698551391No Hit
CTGAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC490.14083697401701542No Hit
CTGAAAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTTCATTCAGC490.14083697401701542No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG490.14083697401701542No Hit
CTGAAAGGGATTTGTTTCAGGGAAGTTCAATTAAAAGAATTCCATTTCTTTAACATTTGCTTTA480.13796275005748448No Hit
CTGAAAGGGGGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCGCCTAGCTCTA470.13508852609795355No Hit
CTGAAAGGGGAGAGTCGTCACCAGCGTCCTTCGGAGCGGAAGGAATATGGCGTCCGCTACTCGC450.1293400781788917No Hit
CTGAAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAG440.1264658542193608No Hit
CTGAAAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC440.1264658542193608No Hit
CTGAAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAAGAAGGT420.12071740630029892No Hit
CTGAAAGGGGTGTTGTTTTTTAAAAATGAGCATCTTCTGTATGTATCCCACAGTAAGTTCACAT410.11784318234076799No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG390.11209473442170614No Hit
CTGAAAGGGAGACAAGGCAGTGAAGGCTGCTCGTGCAGCCTTCCAGCTGGGCTCGCCCTGGCGC390.11209473442170614No Hit
CTGAAAGGGACTCTTACCTGAAAGATTCACAGAAGTGTCAAGTGCTATGCATTGAAACTTGTTT380.10922051046217522No Hit
CTGAAAGGGGGCCAGTCAGCTATTGTAGAGAGCTGAACTCGTATTCCATTCCTTTTTTGGGATC370.10634628650264429No Hit
CTGAAAGGGGGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTACCATGAGTTCCGGTG370.10634628650264429No Hit
CTGAAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT360.10347206254311336No Hit
CTGAAAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTGCCTGGCTC360.10347206254311336No Hit
CTGAAAGGGAGTCTAGACTTTGTGTCTGAGCCCAGCCTGGATCTCCCAGACCACGGGCCGGGTG360.10347206254311336No Hit
CTGAAAGGGATATGGAAAGCTTCAGTTTGCTACAGATCGTACAAACTAAAAATTAAGTAATTTA350.10059783858358243No Hit
CTGAAAGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC350.10059783858358243No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA350.10059783858358243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTCA150.004510833458.44233744
TGCTGAG150.004510833458.44233739
GTCCCGA150.004510833458.44233745
GATCCGA150.004510833458.44233739
GATTCAT150.004510833458.44233745
AGTGAAT150.004510833458.44233741
GAATATG150.004510833458.44233743
GTGAATC150.004510833458.44233742
ATCCGAG150.004510833458.44233740
TGAATCT150.004510833458.44233743
TCCGAGT150.004510833458.44233741
GGAATAT150.004510833458.44233742
TTTGATT150.004510833458.44233742
GGCACGG501.8189894E-1258.44233341
ATAGCAT201.9097478E-458.44233342
GCACGGC501.8189894E-1258.44233342
CCGAGTT201.9097478E-458.44233342
AGCATTT201.9097478E-458.44233344
CATAGCA201.9097478E-458.44233341
GCATTTA201.9097478E-458.44233345
Produced by FastQC (version 0.11.5)