SRR1526703_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526703_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31380
Sequences flagged as poor quality	0
Sequence length	56
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGAAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	728	2.3199490121096242	No Hit
CTGAAAGGGGATCACACCCACAGCATGCTCTCTCCCTATAGGCACGGCCTCCGCCG	279	0.8891013384321224	No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	268	0.8540471637985979	No Hit
CTGAAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	196	0.6246016571064372	No Hit
CTGAAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	191	0.6086679413639261	No Hit
CTGAAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	148	0.4716379859783302	No Hit
CTGAAAGGGTACGAGGTGGAGATTCGGGATGCAAAGACGAGGGAGAAGCTGTGCTT	134	0.42702358189929895	No Hit
CTGAAAGGGGTCATAGTTAAAGTCAAAGACAGAGGATATGTGCTCCCAAGGGAGCA	128	0.4079031230082855	No Hit
CTGAAAGGGACCATAACTGCCCAACTGTCCAATATGTCAATAGAGTTTGCTCTAAG	128	0.4079031230082855	No Hit
CTGAAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	111	0.35372848948374763	No Hit
CTGAAAGGGGGGAGAAGGTGGCTTCTGTGTTTGACTTCAATGCTTGCCAAGAGCAA	109	0.34735500318674317	No Hit
CTGAAAGGGTGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCC	106	0.33779477374123645	No Hit
CTGAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98	0.3123008285532186	No Hit
CTGAAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	95	0.3027405991077119	No Hit
CTGAAAGGGGACACCAAACCTCAGCTCAAACCCTCCAGAAAGTGAATCTACATCCC	94	0.2995538559592097	No Hit
CTGAAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT	90	0.28680688336520077	No Hit
CTGAAAGGGTACCCAGACCTCTCTACCTTGTGAAACAATTGTCAGCCCTTTGGTGC	90	0.28680688336520077	No Hit
CTGAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	85	0.2708731676226896	No Hit
CTGAAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	81	0.25812619502868067	No Hit
CTGAAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	76	0.24219247928616955	No Hit
CTGAAAGGGTGGCATTATTGTTGACTATGCTTGCCTTCCTATTCTAGTATGAACTA	76	0.24219247928616955	No Hit
CTGAAAGGGGGGAGCGTGGAAACTACCCGGTCAAAGGATGCTGAGTCCCGAGCGCC	76	0.24219247928616955	No Hit
CTGAAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	73	0.23263224984066286	No Hit
CTGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	70	0.22307202039515617	No Hit
CTGAAAGGGGAACAAAAGCGTTTTCAGCTACTGAGTGTTGTTTTTTGTTTGTTTGT	69	0.21988527724665394	No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG	67	0.21351179094964948	No Hit
CTGAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	65	0.207138304652645	No Hit
CTGAAAGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACT	64	0.20395156150414276	No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	59	0.1880178457616316	No Hit
CTGAAAGGGGGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTACCATGAG	59	0.1880178457616316	No Hit
CTGAAAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA	54	0.17208413001912046	No Hit
CTGAAAGGGCTGTAATGGCCTATAGGTGTGCACTTTAGTGTTTTTGAATTATTTTA	52	0.165710643722116	No Hit
CTGAAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	52	0.165710643722116	No Hit
CTGAAAGGGGAGTCGGAAAACCAATGAGGAAGACCTTTGTATCGCAAGCAAGTGCT	52	0.165710643722116	No Hit
CTGAAAGGGGTGTTGTTTTTTAAAAATGAGCATCTTCTGTATGTATCCCACAGTAA	51	0.16252390057361377	No Hit
CTGAAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC	50	0.15933715742511154	No Hit
CTGAAAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	48	0.15296367112810708	No Hit
CTGAAAGGGGACCGCAAAGCAGGAGCTGGGGACTAGACCGCACTCGGACCTGCTCC	47	0.14977692797960485	No Hit
CTGAAAGGGACAGTGGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	47	0.14977692797960485	No Hit
CTGAAAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG	45	0.14340344168260039	No Hit
CTGAAAGGGACCTGAGCCTCTAGACAGACTTCTGTGGACATGCCGGGCTGGCAGCA	45	0.14340344168260039	No Hit
CTGAAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	45	0.14340344168260039	No Hit
CTGAAAGGGGTCCCTTGCTCTGTACCGGTTCTCTCTGAGGTGAGGAGAAAAGTGAT	44	0.14021669853409816	No Hit
CTGAAAGGGGGCTTTTCTTCTTTCCATGCAACTCTCCATCCCATTGTCTCCTCCAG	44	0.14021669853409816	No Hit
CTGAAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG	43	0.13702995538559593	No Hit
CTGAAAGGGAGGAGCTGGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACC	43	0.13702995538559593	No Hit
CTGAAAGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTA	42	0.13384321223709367	No Hit
CTGAAAGGGATTTGTTTCAGGGAAGTTCAATTAAAAGAATTCCATTTCTTTAACAT	41	0.13065646908859146	No Hit
CTGAAAGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC	40	0.12746972594008923	No Hit
CTGAAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	40	0.12746972594008923	No Hit
CTGAAAGGGAGACAAGGCAGTGAAGGCTGCTCGTGCAGCCTTCCAGCTGGGCTCGC	40	0.12746972594008923	No Hit
CTGAAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	39	0.12428298279158699	No Hit
CTGAAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC	39	0.12428298279158699	No Hit
CTGAAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG	39	0.12428298279158699	No Hit
CTGAAAGGGGGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACTGAGAAGC	39	0.12428298279158699	No Hit
CTGAAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT	38	0.12109623964308477	No Hit
CTGAAAGGGGCTCCTCGCCCGGCCCCAGCTGCCAGCACCATGTCCGCAGGGGGAGA	38	0.12109623964308477	No Hit
CTGAAAGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAA	38	0.12109623964308477	No Hit
CTGAAAGGGGGACATTGCTGTAATCCCTTATAAGTTAATGTTTTCCAACATGAGTC	37	0.11790949649458254	No Hit
CTGAAAGGGAATTCCAGGCGTGGAGAATGTCAGTCATTTAAGCTGAAGCTCAGCAG	37	0.11790949649458254	No Hit
CTGAAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	36	0.11472275334608031	No Hit
CTGAAAGGGGTACATACATGAGGAAACCCCTATGGATAACATTTGATTCATCTGTA	36	0.11472275334608031	No Hit
CTGAAAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT	36	0.11472275334608031	No Hit
CTGAAAGGGGAGCACTTTTTATATTGTCTTTTGTGTAAATATTTTAGCTACTGGTT	35	0.11153601019757808	No Hit
CTGAAAGGGGGCCAGAGTCGTGAGGAACGGAGCGGAGGCAGCTGCGAGGCCGCGGG	35	0.11153601019757808	No Hit
CTGAAAGGGGGTCTTCTTCCTCTCATTGAAGAACAGGCCTGGCAAAGATGCACTCT	34	0.10834926704907585	No Hit
CTGAAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	33	0.10516252390057361	No Hit
CTGAAAGGGACTCTTACCTGAAAGATTCACAGAAGTGTCAAGTGCTATGCATTGAA	32	0.10197578075207138	No Hit
CTGAAAGGGGTATTCAGAGAGACACAAGTATGTAGAGTCATTCATGGCCCAGAGCG	32	0.10197578075207138	No Hit
CTGAAAGGGTACTTGTGGTGTGACCATGTTCATTATAATCTCAAAGGAGAAAAAAA	32	0.10197578075207138	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCCA	20	3.9369185E-4	50.46452	49
CCTCCAG	20	3.9369185E-4	50.46452	50
CAAGGAG	95	0.0	50.46452	49
TTGAGCG	15	0.00805447	50.464516	48
ATTATAT	15	0.00805447	50.464516	50
AGAAACA	15	0.00805447	50.464516	50
TGAGCGC	15	0.00805447	50.464516	49
CTATTAT	15	0.00805447	50.464516	48
ATCCCAT	20	4.096599E-4	50.060802	38
TCCCATT	20	4.096599E-4	50.060802	39
CATTGTC	20	4.096599E-4	50.060802	42
TGCACCG	95	0.0	50.060802	38
CCAGCAC	20	4.096599E-4	50.060802	32
CCTGCAC	95	0.0	50.060802	36
CATCCCA	20	4.096599E-4	50.060802	37
ACTCTCC	20	4.096599E-4	50.060802	31
ATTGTCT	20	4.096599E-4	50.060802	43
CGGCCAA	95	0.0	50.060802	45
TCTCCAT	20	4.096599E-4	50.060802	33
CTGCACC	95	0.0	50.060802	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content