FastQCFastQC Report
Fri 17 Jun 2016
SRR1526702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407674
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC10480.2570681475885143No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC7240.1775928805859584No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC7080.1736681760426223No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC6740.16532817888803308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTA351.614535E-858.1046644
ATACGGT1000.058.104663
TATACGA2350.058.104662
TATACCG303.7091013E-758.1046562
TATACGG404100.057.493562
TACGGGA97700.057.450464
ATACGGG398550.057.4486053
TACGGGG245350.057.4178734
GTATACG408100.057.3156851
ACGGGAT24500.057.2745935
ACGGGGG124150.057.0282145
TACGGGT20750.056.8445634
TACGGGC35550.056.7153784
ACGGGGC45750.056.6441045
ACGGGTA3600.056.4906435
ACGGGGT27950.056.4415555
ACGGGGA50300.056.4296765
ACGGGAC18200.056.348755
ACGGGAG37600.056.172995
CGGGTAT1350.055.9526376
Produced by FastQC (version 0.11.5)