FastQCFastQC Report
Fri 17 Jun 2016
SRR1526699_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526699_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547126
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC56551.0335827579022017No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC35450.6479311895248991No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC16320.2982859524131553No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT16170.29554435358582853No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC16170.29554435358582853No Hit
GTATACGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC11830.21622076084850655No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG10340.18898754583039373No Hit
GTATACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC8500.1553572668818517No Hit
GTATACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA8020.14658415063440597No Hit
GTATACGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT7640.13963876693851143No Hit
GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG7420.1356177553250988No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT7310.13360724951839248No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC6830.12483413327094672No Hit
GTATACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC6820.1246513600157916No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT6280.11478160423741515No Hit
GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC6120.11185723215493323No Hit
GTATACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG5980.10929840658276156No Hit
GTATACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG5950.1087500868172962No Hit
GTATACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5920.10820176705183084No Hit
GTATACGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC5900.10783622054152059No Hit
GTATACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5550.10143915661109142No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATCG203.9095385E-450.68713450
CGAAATT150.00799179350.67770449
AATCGTA150.00827763350.22918743
CCACGGT150.00831086550.178340
TATACTA204.1767923E-450.01248630
TACGCGC150.00849080349.90711624
CCAATCA150.00851234149.87513723
ATATACG150.00854009549.8340821
TTAGTAA204.2510947E-449.8340820
AGACTAC150.00856791549.7930915
ATTACGC150.00856791549.7930910
TACGGTA252.1378222E-549.793094
TCCGTAT150.00856791549.7930917
TATACGA2000.049.793092
TATACGG545350.049.409612
TACGGGA129150.049.4075434
GTATACG547650.049.4021341
ATACGGG537050.049.3990483
TACGGGG337950.049.3510744
TACGGGT27000.049.3320434
Produced by FastQC (version 0.11.5)