##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526699_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 547126 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.64215738239455 39.0 38.0 40.0 36.0 40.0 2 38.52569974740736 39.0 39.0 40.0 36.0 40.0 3 38.47659222921229 39.0 38.0 40.0 36.0 40.0 4 38.36069936358353 39.0 38.0 40.0 35.0 40.0 5 38.253839152224536 39.0 38.0 40.0 35.0 40.0 6 38.417689892273444 39.0 38.0 40.0 36.0 40.0 7 38.76983729524826 39.0 39.0 40.0 38.0 40.0 8 38.73186066829213 39.0 39.0 40.0 38.0 40.0 9 38.70815132163341 39.0 39.0 40.0 38.0 40.0 10 37.94911409803226 39.0 38.0 40.0 35.0 40.0 11 37.81160646724886 39.0 38.0 40.0 35.0 40.0 12 37.66148017092955 39.0 38.0 40.0 34.0 40.0 13 37.43468597727032 39.0 37.0 40.0 33.0 40.0 14 37.64867507667338 39.0 38.0 40.0 33.0 40.0 15 37.629014157616346 39.0 38.0 40.0 33.0 40.0 16 37.43457265785212 39.0 37.0 40.0 33.0 40.0 17 37.24293489982198 39.0 37.0 40.0 33.0 40.0 18 37.1556497040901 39.0 36.0 40.0 33.0 40.0 19 37.05206296173093 39.0 36.0 40.0 33.0 40.0 20 36.97239027207627 39.0 36.0 40.0 33.0 40.0 21 36.7691226518206 38.0 36.0 40.0 32.0 40.0 22 36.63000661639184 38.0 36.0 40.0 32.0 40.0 23 36.520563818937504 38.0 36.0 40.0 31.0 40.0 24 36.35551043086967 38.0 35.0 40.0 31.0 40.0 25 36.144566333897494 38.0 35.0 40.0 31.0 40.0 26 35.60865321699207 38.0 35.0 39.0 29.0 40.0 27 35.34354792131977 38.0 35.0 39.0 29.0 40.0 28 35.05599624218188 38.0 34.0 39.0 28.0 40.0 29 34.893964096021755 38.0 34.0 39.0 28.0 40.0 30 34.523215858869804 38.0 33.0 39.0 27.0 40.0 31 33.6777451629058 37.0 33.0 39.0 24.0 40.0 32 33.534633704119344 37.0 33.0 39.0 23.0 40.0 33 33.24034500279643 36.0 32.0 39.0 23.0 40.0 34 33.32607114266184 36.0 32.0 39.0 23.0 40.0 35 33.07782485204505 36.0 32.0 39.0 22.0 40.0 36 32.723263014369635 36.0 32.0 39.0 16.0 40.0 37 32.47970120228247 36.0 32.0 39.0 15.0 40.0 38 32.232430920848216 36.0 31.0 39.0 12.0 40.0 39 32.00928122589678 36.0 31.0 39.0 10.0 40.0 40 31.688835844028613 36.0 31.0 39.0 4.0 40.0 41 31.195713601620103 36.0 30.0 39.0 4.0 40.0 42 30.9554581577187 36.0 30.0 39.0 4.0 40.0 43 30.780582534918832 35.0 30.0 39.0 4.0 40.0 44 30.472741196726165 35.0 29.0 39.0 4.0 40.0 45 30.017080160694245 35.0 29.0 38.0 4.0 40.0 46 29.22132744559754 35.0 28.0 38.0 4.0 40.0 47 28.916571319951895 34.0 27.0 38.0 4.0 40.0 48 28.56460669023223 34.0 26.0 38.0 4.0 39.0 49 28.283744512233014 34.0 25.0 38.0 4.0 39.0 50 27.814474179622245 33.0 23.0 38.0 4.0 39.0 51 26.80040246670785 33.0 14.0 37.0 4.0 39.0 52 26.472772268179543 33.0 4.0 37.0 4.0 39.0 53 26.134660023468086 33.0 4.0 36.0 4.0 39.0 54 25.734220271016184 33.0 4.0 36.0 4.0 39.0 55 25.389537327781902 33.0 4.0 36.0 4.0 39.0 56 14.899664793850045 18.0 4.0 22.0 4.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.6383516642449365 1 2 -0.8132420200409598 1 3 -0.847550423225556 1 4 -1.0214002884713267 1 5 -1.403235488360238 1 6 -0.7632750071612122 1 7 -0.7291483381433324 1 8 -0.7876217141659865 1 9 -0.7198495983223197 1 10 -0.614870622890173 1 11 -0.9557273889434228 1 12 -1.2388654389779319 1 13 -0.6061421472700772 1 14 -0.8089849298360008 1 15 -1.0509644041941613 1 16 -0.5997185178741518 1 17 -0.7337003369366357 1 18 -0.643726769739672 1 19 -0.8345136881258242 1 20 -0.7858587528607259 1 21 -0.540910826174418 1 22 -0.45934459232331193 1 23 -0.5251007111470543 1 24 -0.7651827077169884 1 25 -0.8040453895189046 1 26 -0.14228315829226545 1 27 -0.5972310709600777 1 28 7.750508385697685E-4 1 29 -0.1407798149541577 1 30 0.09053459478079873 1 31 0.19165852186594634 1 32 0.15576822997493167 1 33 0.2557327447180455 1 34 -0.7723031823963566 1 35 -0.9220077502881239 1 36 0.30836511925472365 1 37 0.6041569370771995 1 38 0.752823309513424 1 39 0.5357167035651216 1 40 0.7347769245794034 1 41 0.5928933651956889 1 42 0.7979325539149258 1 43 0.9323887074316133 1 44 1.1022202764148332 1 45 1.6914947584194238 1 46 1.8161627081563552 1 47 2.0639479216746643 1 48 1.5037706729986269 1 49 1.7712423146954848 1 50 1.6931028626944382 1 51 1.9059857699218483 1 52 1.605784238586537 1 53 1.6803791248790603 1 54 2.3360083872210886 1 55 1.518271547138351 1 56 1.810599370488232 2 1 -0.10649450713157904 2 2 -0.2816228320530243 2 3 -0.19539603499811875 2 4 -0.37732149766154066 2 5 -0.23397802879580354 2 6 -0.21062625726676032 2 7 0.015211409762926564 2 8 -0.07886137978098873 2 9 -0.06800994345432088 2 10 -0.3887078366555343 2 11 -0.4735136619631035 2 12 -0.2776812739041219 2 13 -0.38620110220179527 2 14 -0.3162890467018684 2 15 -0.2701416451340606 2 16 -0.35332451776667995 2 17 -0.646031743487697 2 18 -0.34577791007237124 2 19 -0.22499031725477892 2 20 -0.34344344300189533 2 21 -0.36631825234641724 2 22 -0.3510801548899991 2 23 -0.18526928540493515 2 24 -0.37311322073236397 2 25 -0.46234091646699227 2 26 0.08055189839812726 2 27 0.15590282889330354 2 28 0.11906105902163233 2 29 -0.013097864528504033 2 30 0.46036548317328396 2 31 0.4757053787543839 2 32 0.9479252281863211 2 33 0.3490166418846812 2 34 0.8506846351197055 2 35 0.5504492425497745 2 36 0.762955236627235 2 37 0.8609102705640623 2 38 0.9704499129263624 2 39 0.4014790875551739 2 40 0.5088329164116949 2 41 0.6666906615593646 2 42 0.2747190495508818 2 43 0.48529845572168995 2 44 0.8115217986495935 2 45 0.4683679976398061 2 46 0.2804826921893948 2 47 0.4408104139022839 2 48 0.4363332934225177 2 49 0.6549483383849264 2 50 0.5528853128552598 2 51 0.7717826978172049 2 52 0.9451243732310033 2 53 0.7061680692940762 2 54 0.77273162912822 2 55 0.7884701362117283 2 56 1.0045058255926715 3 1 -0.2527718461128856 3 2 -0.2820309863132735 3 3 -0.23043111420211204 3 4 -0.48019107950565854 3 5 -0.6443017576342882 3 6 -0.3385844563872098 3 7 -0.04952332673856574 3 8 -0.012630077658585037 3 9 -0.08169230383614234 3 10 -0.2661586007497263 3 11 -0.4443986580654098 3 12 -0.0828482862149329 3 13 0.06003284740770454 3 14 -0.17061474644741992 3 15 0.039452553927553424 3 16 -0.05825840413065464 3 17 -0.4798835430435062 3 18 -0.34759070331833897 3 19 -0.25003480974621795 3 20 -0.002237512630806293 3 21 0.14538216978069585 3 22 0.11577477752553733 3 23 0.03307118143528953 3 24 0.04910213763443494 3 25 0.17053719616887975 3 26 -0.0745540746269242 3 27 0.22969785455921965 3 28 0.533620767209726 3 29 0.5300635292263678 3 30 0.3672217393323649 3 31 0.7688079394627323 3 32 0.9189977546244279 3 33 0.735090124359786 3 34 0.6967442918288782 3 35 1.2453153238276258 3 36 1.5982741852806939 3 37 1.033614678630073 3 38 1.8009666141574456 3 39 1.4990389869506657 3 40 1.4235029365620129 3 41 1.670308893920481 3 42 1.6167412884424621 3 43 1.5806962406574883 3 44 1.912368994519511 3 45 1.7163419346001945 3 46 2.2575194187186867 3 47 1.930474183041408 3 48 2.324381360021526 3 49 2.221304660731189 3 50 2.155383511093209 3 51 2.042455159538953 3 52 2.221139610990054 3 53 1.9112343300127215 3 54 1.7504118421038477 3 55 2.213840416698279 3 56 0.9033276938360633 4 1 -0.030943771637417683 4 2 -0.23619623798543188 4 3 -0.12471256768446892 4 4 -0.5282664408829518 4 5 -0.17787773176572585 4 6 -0.0976281169657085 4 7 0.04725860661149994 4 8 0.059084305083899835 4 9 0.06597037131556505 4 10 -0.04419394854307512 4 11 -0.03502721093206418 4 12 0.12418140652208365 4 13 0.2116119812834114 4 14 -0.12544443032533792 4 15 0.2399338981477257 4 16 0.1692173440938376 4 17 -0.09636958258460737 4 18 0.0267171798006558 4 19 0.11862539962375962 4 20 0.07876784712232165 4 21 0.021974961429521045 4 22 0.16448504078834247 4 23 -0.0234978383057296 4 24 0.551265233593206 4 25 0.3236334502477547 4 26 0.2687728112531502 4 27 0.40335459411655705 4 28 0.22604033399992574 4 29 0.4719183286416708 4 30 0.7735626373494071 4 31 0.47022318006101926 4 32 0.7387631362299913 4 33 0.2756684756820036 4 34 0.9459765035022158 4 35 0.9994938641439006 4 36 0.9891005912930666 4 37 0.7789009012591563 4 38 1.1006698344388681 4 39 1.379721727954987 4 40 1.205709442091564 4 41 1.5209297689038728 4 42 1.4165220258145652 4 43 1.4454621390436557 4 44 1.4990700147920784 4 45 1.4486864943769469 4 46 1.5799277639871896 4 47 1.1285598800307923 4 48 0.882880924449303 4 49 1.1843326997702732 4 50 1.472646198057049 4 51 1.6225893748445195 4 52 1.7179429538223054 4 53 1.7920831790684915 4 54 1.3794593416239813 4 55 1.763407059248518 4 56 1.0366275594731071 5 1 -0.22895222496502043 5 2 -0.265138139267755 5 3 -0.37545440185295575 5 4 -0.2274493864264855 5 5 -0.2924350254943562 5 6 -0.07561628532341302 5 7 -0.05164004771828701 5 8 0.020463683036624047 5 9 0.07390714856925484 5 10 0.02521232019130082 5 11 -0.02693961605835682 5 12 -0.1445642810365939 5 13 0.27307393188309703 5 14 0.011196333415085746 5 15 -0.2542992131066555 5 16 -0.17503518492912917 5 17 0.1327713979561551 5 18 -0.9232830087213699 5 19 0.045863438843881 5 20 -0.074730556096128 5 21 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0.06939431554795306 6 7 0.04774409213555231 6 8 0.0629758953957662 6 9 0.08094721442667918 6 10 -0.1746315790654691 6 11 -0.09716165189163206 6 12 -0.13727181347992 6 13 0.48349928699287403 6 14 0.17906287892893857 6 15 0.022740775088706755 6 16 -0.053901784806420494 6 17 0.146777750712765 6 18 0.09930111870654201 6 19 0.115685515061422 6 20 0.15836435470900057 6 21 0.14833220171402672 6 22 0.08248807699940386 6 23 -0.14063855975263095 6 24 0.2786419934782316 6 25 -0.11281032975794858 6 26 0.4274379572455871 6 27 0.26731076170924695 6 28 0.19997823808956383 6 29 0.19388153962751176 6 30 0.3514907700796073 6 31 0.7832919880014018 6 32 0.3848518953135738 6 33 0.8354140196184474 6 34 0.5065812797550393 6 35 0.5999046003094861 6 36 0.6768910156690637 6 37 0.460730642256479 6 38 0.45999462425446325 6 39 0.9480673011746816 6 40 0.6690398727865805 6 41 0.5470766321279577 6 42 1.240163629612141 6 43 1.014062014204523 6 44 1.0902680851797726 6 45 1.4245624875030884 6 46 1.2623778475985858 6 47 1.4245056905989664 6 48 1.481858322401017 6 49 1.0262223943767594 6 50 1.5456925041287022 6 51 1.3103682400413703 6 52 1.8308838401801637 6 53 1.2759966547595383 6 54 1.606924721882443 6 55 1.4146858897678314 6 56 1.2886870198906255 7 1 -0.05936158169016181 7 2 -0.06045977669465685 7 3 0.053395500681155283 7 4 0.04545509700431438 7 5 0.07208739165964317 7 6 0.053038685436817445 7 7 0.09066014721349092 7 8 0.12182820545378092 7 9 0.12069077430025033 7 10 0.15581660869032987 7 11 -0.017193027364399427 7 12 0.06791135249167013 7 13 0.22823962389089303 7 14 -0.14085045021064246 7 15 -0.10810038269730171 7 16 -0.11265701728780186 7 17 0.05869732388207893 7 18 -0.05778593269797483 7 19 0.14722796126103077 7 20 -0.10591963991679165 7 21 -0.03056235650533523 7 22 0.024439569275905626 7 23 -0.4076717913486618 7 24 0.07076783708237144 7 25 0.20636520979922324 7 26 0.3981554770299951 7 27 -0.23045645402819304 7 28 0.3567313426304608 7 29 0.18818891053375353 7 30 0.832234461978679 7 31 0.5760896084358436 7 32 0.5085287279440678 7 33 0.626281737829423 7 34 0.6339159954730604 7 35 0.8963911686559811 7 36 0.6260184824102879 7 37 0.9404491949463036 7 38 0.7579179407351262 7 39 1.0764388054111507 7 40 0.9822354336231562 7 41 1.3819437911720946 7 42 1.3720609914319155 7 43 0.6572553228413192 7 44 1.4380077308854489 7 45 0.8630541349483956 7 46 0.9441070710556332 7 47 1.4109847257255161 7 48 1.1450962090610055 7 49 1.2574340001952002 7 50 1.4623378137285101 7 51 1.2583346589660636 7 52 1.6656733371629926 7 53 1.5780088787679851 7 54 1.68313061953938 7 55 1.9836498002079423 7 56 1.5772559410869302 8 1 -0.8020161980289942 8 2 -0.8193468934765065 8 3 -0.9357592042630216 8 4 -0.7944173112891448 8 5 -0.8884300635946545 8 6 -0.529736434180407 8 7 -0.4619182685228225 8 8 -0.5459017254340495 8 9 -0.5524263619747813 8 10 -0.4182751864541032 8 11 -0.16165519287919494 8 12 -0.20665891155227456 8 13 0.031642069398607475 8 14 -0.3001274305402575 8 15 -0.3539625127699608 8 16 -0.0809008592158591 8 17 0.02488552164922453 8 18 0.01777671128571967 8 19 0.08570040499663634 8 20 -0.2719306269804065 8 21 0.10231022899883158 8 22 0.07026625720786228 8 23 0.04630658894550521 8 24 0.1538442148955923 8 25 0.4875277120143551 8 26 0.8395873395144875 8 27 0.6613386903985301 8 28 1.209362921577835 8 29 1.06577175653765 8 30 1.207992037736254 8 31 1.3261870743227817 8 32 1.5506694332426676 8 33 1.8761842719424848 8 34 2.081373021877461 8 35 2.1380711261254177 8 36 2.318522469651114 8 37 2.0573019328516793 8 38 1.8308224241659303 8 39 2.0176757361217668 8 40 2.4915035534175907 8 41 2.243861210984253 8 42 2.2459224126617556 8 43 1.8607640947711417 8 44 2.204550350059648 8 45 2.514622449674601 8 46 2.6456488041763144 8 47 2.1818158439250546 8 48 2.6855888548168103 8 49 2.4919192031014568 8 50 2.8421712356671947 8 51 3.085731433731258 8 52 3.0149556338137096 8 53 3.382970778466728 8 54 3.3952341105902413 8 55 2.6198007837273494 8 56 3.2151790316415987 9 1 0.001067268212366912 9 2 0.08963768919228698 9 3 -0.26629260848064007 9 4 -0.14167940591966044 9 5 -0.07801007422730066 9 6 0.06278527413077484 9 7 -0.016552328420040396 9 8 -0.008776083045248129 9 9 0.09340032595716963 9 10 0.052502768534381516 9 11 0.160194886865618 9 12 0.24724858446047904 9 13 0.48944820088894403 9 14 0.12815539774257445 9 15 0.41626608903759177 9 16 0.23042290473948412 9 17 0.26337568645517706 9 18 0.25020627003106455 9 19 0.14138000804465634 9 20 0.3424234400052413 9 21 0.48600684427439234 9 22 0.6813596472486196 9 23 0.7326790858443175 9 24 0.7588769988757491 9 25 0.7092891864074105 9 26 1.0453289663087517 9 27 1.0872823373948037 9 28 0.8089730580300767 9 29 1.100469612288137 9 30 1.4209284190936842 9 31 1.8080584193520224 9 32 1.578704166540561 9 33 1.5054240380243513 9 34 1.8405436757849571 9 35 1.859354131618943 9 36 2.2244590282192505 9 37 2.152924828474575 9 38 2.054214237031424 9 39 1.902949526658709 9 40 2.4247305603288076 9 41 2.2825285864937293 9 42 2.284341693186299 9 43 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120 51 0.7530863455751486 120 52 0.5833449376739566 120 53 0.5241663008245752 120 54 0.7021550231223728 120 55 0.48270639292554307 120 56 0.5838031485623105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 176.0 5 24.0 6 72.0 7 133.0 8 247.0 9 329.0 10 386.0 11 662.0 12 780.0 13 1101.0 14 1560.0 15 2111.0 16 2502.0 17 3320.0 18 4371.0 19 4937.0 20 5103.0 21 6348.0 22 6357.0 23 6882.0 24 8919.0 25 11229.0 26 9902.0 27 11033.0 28 12079.0 29 13481.0 30 15262.0 31 18041.0 32 21520.0 33 26824.0 34 35131.0 35 47780.0 36 65752.0 37 82385.0 38 81580.0 39 38807.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.36157301974316 0.5852399630066931 0.022298337128924598 0.03088868012121522 8 99.55665492944871 0.39453140711326595 0.020476143464636606 0.028337519973381017 9 98.4634252439109 0.8237590609841243 0.2644729002094582 0.44834279489550855 10 61.9844423405212 24.642769672799318 5.203189027755947 8.169598958923538 11 41.95742845340927 21.61896893951302 16.871981956624253 19.55162065045346 12 33.899503953385505 15.597321275172446 25.070459089862297 25.432715681579747 13 27.17874858807661 15.414365246762172 27.86798653326656 29.538899631894665 14 24.414120330600262 17.56670309946886 31.91166203031843 26.10751453961245 15 27.73949693489251 18.53759463085286 28.941413860792576 24.781494573462055 16 33.406747257487304 18.881025577289325 23.725065158665462 23.987162006557906 17 32.63818571956003 19.199964907535012 23.911311105668528 24.250538267236433 18 32.78805978878723 20.09537108453994 22.69257904029419 24.42399008637864 19 31.365169997404617 22.180996699115013 21.580038236164977 24.873795067315392 20 31.442222104433938 22.677067778425613 22.60852718137304 23.272182935767407 21 30.72345309855499 21.240811074597076 25.216494920731243 22.81924090611669 22 29.45153401593052 19.155551006532313 26.964355559779644 24.428559417757516 23 31.59838867098255 19.170172866944725 25.083618764233467 24.147819697839253 24 31.72369671711633 20.140016085398845 22.679681216641075 25.456605980843754 25 29.280220553687748 19.906140365310325 22.398704173956602 28.414934907045325 26 26.47458513718411 19.02342399683949 25.781211419541393 28.720779446435007 27 26.251744440197022 17.97573604859375 24.916550370653532 30.855969140555693 28 27.10302089984796 21.531252801618855 23.33453478947378 28.031191509059404 29 30.423606394100233 20.284296152811013 23.19481646530243 26.097280987786327 30 30.302080756438798 18.132908202999733 25.652950716033097 25.91206032452837 31 32.73049261833308 18.14828598299091 24.740546278184254 24.38067512049176 32 36.05424342810839 18.24125015812139 23.132838466855006 22.571667946915213 33 35.01309236535401 18.544033622074387 24.822867998151665 21.62000601441994 34 29.005770084332 20.607234431226683 29.448338853979816 20.938656630461498 35 28.438215606834593 24.375403675650283 26.986818155128883 20.199562562386237 36 28.80021292021916 26.36301061847817 23.594313561733095 21.24246289956957 37 30.5041716000771 24.75645014731393 22.625767546878873 22.1136107057301 38 31.27570958453311 23.763736263736263 22.505068460950813 22.45548569077981 39 32.125252844442564 21.323811745944845 22.62767151625829 23.923263893354303 40 30.006287851209585 20.1529308373138 23.12403246149151 26.716748849985105 41 25.959040012945962 21.943401881945352 24.507584603870182 27.589973501238507 42 25.228726672086697 19.755289131850304 26.006783626591524 29.00920056947147 43 25.300351276205408 17.824553268303994 27.035203064880292 29.839892390610306 44 27.104160571450652 17.79747874264664 25.790906029709692 29.307454656193016 45 27.976970535832606 19.00390941351435 25.330385934946182 27.688734115706858 46 27.030087648228623 20.91920159092583 24.999631730131842 27.05107903071371 47 26.469575084598084 19.735991349535148 26.314286714278605 27.48014685158816 48 27.132194393763477 19.307605831075726 28.147103813051732 25.413095962109068 49 29.391032313811905 18.311221491199788 25.78748680382703 26.510259391161284 50 26.036205434502087 19.27773476385356 26.410795266010396 28.275264535633966 51 25.03736576429805 20.14028517772466 28.134255052901814 26.68809400507548 52 25.39132133975596 19.654829783743228 27.769943055394254 27.183905821106556 53 25.724567545494605 18.96181421647521 29.171298626358936 26.14231961167125 54 25.527856050910458 18.52413918645063 28.81433695484595 27.13366780779297 55 26.41176405493237 18.56789267310225 25.45546428031295 29.56487899165243 56 26.17759820310584 19.640252347082352 24.038622897491834 30.143526552319972 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 4.5 5 7.0 6 9.0 7 11.0 8 70.0 9 129.0 10 204.5 11 280.0 12 373.0 13 452.5 14 439.0 15 696.5 16 954.0 17 1203.0 18 1452.0 19 1440.5 20 1429.0 21 1693.5 22 2278.0 23 2598.0 24 3196.0 25 3794.0 26 4479.5 27 5165.0 28 5609.5 29 6584.5 30 7115.0 31 6729.0 32 6343.0 33 6550.5 34 6758.0 35 6776.0 36 6794.0 37 6976.5 38 7906.0 39 8653.0 40 8856.5 41 9060.0 42 10011.0 43 10962.0 44 12295.0 45 13628.0 46 13795.0 47 15190.5 48 16419.0 49 18022.5 50 19626.0 51 20783.0 52 21940.0 53 24769.5 54 30010.0 55 32421.0 56 37111.5 57 41802.0 58 50025.0 59 58248.0 60 57492.5 61 56737.0 62 47667.5 63 34609.5 64 30621.0 65 28427.5 66 26234.0 67 22251.5 68 18269.0 69 16124.5 70 13980.0 71 12051.5 72 8786.0 73 7449.0 74 6136.5 75 4824.0 76 3871.5 77 2919.0 78 2417.0 79 1527.0 80 1139.0 81 872.5 82 606.0 83 440.0 84 274.0 85 196.0 86 118.0 87 90.0 88 40.0 89 18.0 90 12.5 91 7.0 92 6.5 93 6.0 94 3.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.02705044176295771 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 9.138662757755984E-4 21 0.0 22 0.0 23 0.0 24 0.008407569737135506 25 0.025405482466561633 26 0.06927106370379035 27 0.07109879625534155 28 0.10363243567295284 29 0.17089299357003687 30 0.2578930630238738 31 0.3021241907714128 32 0.30322083030234354 33 0.3235086616245618 34 0.34726918479472735 35 0.3900381265010253 36 0.42421672521503273 37 0.4333553879727887 38 0.471920544810519 39 0.5152378062822823 40 0.5885298815994853 41 0.6080866199010831 42 0.6331265558573345 43 0.6724228057156852 44 0.6702295266538238 45 0.6985593812028673 46 0.7395005903576142 47 0.7795279332365853 48 0.8254040202805204 49 0.7959775262005462 50 0.8528200085537884 51 0.8255867935356755 52 0.8533683283192537 53 0.8588515259739073 54 1.602738674455244 55 0.9014376944250502 56 0.8877297002884162 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 547126.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.781693183306956 #Duplication Level Percentage of deduplicated Percentage of total 1 66.9316586876154 23.279964167224602 2 12.48564826133236 8.685439740407041 3 6.1544172563920965 6.421831582016391 4 3.692196306022591 5.136833563544683 5 2.3913966288332436 4.158841191183623 6 1.670132155223707 3.4854014519139693 7 1.186812489294329 2.8895543508126487 8 0.8732563046641146 2.4298666287372526 9 0.6617180253978872 2.071410599992773 >10 3.5809360130214047 22.948796023264492 >50 0.23584510751160281 5.658840315796794 >100 0.12270319782691791 7.7059182712943235 >500 0.00956128814235724 2.118947389136028 >1k 0.003187096047452413 1.9635689991924061 >5k 5.311826745754022E-4 1.0447857254829749 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 5655 1.0335827579022017 No Hit GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 3545 0.6479311895248991 No Hit GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 1632 0.2982859524131553 No Hit GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 1617 0.29554435358582853 No Hit GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 1617 0.29554435358582853 No Hit GTATACGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC 1183 0.21622076084850655 No Hit GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1034 0.18898754583039373 No Hit GTATACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 850 0.1553572668818517 No Hit GTATACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA 802 0.14658415063440597 No Hit GTATACGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT 764 0.13963876693851143 No Hit GTATACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 742 0.1356177553250988 No Hit GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 731 0.13360724951839248 No Hit GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 683 0.12483413327094672 No Hit GTATACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 682 0.1246513600157916 No Hit GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT 628 0.11478160423741515 No Hit GTATACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 612 0.11185723215493323 No Hit GTATACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 598 0.10929840658276156 No Hit GTATACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG 595 0.1087500868172962 No Hit GTATACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 592 0.10820176705183084 No Hit GTATACGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC 590 0.10783622054152059 No Hit GTATACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 555 0.10143915661109142 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.310930206204787E-4 0.0 0.0 0.0 0.0 8 0.0012794127860858377 0.0 0.0 0.0 0.0 9 0.0014621860412409573 0.0 0.0 0.0 0.0 10 0.001644959296396077 0.0 0.0 0.0 0.0 11 0.0023760523170165554 0.0 0.0 0.0 0.0 12 0.002741598827326795 0.0 0.0 0.0 0.0 13 0.004021011613412633 0.0 0.0 0.0 0.0 14 0.004752104634033111 0.0 0.0 0.0 0.0 15 0.004934877889188231 0.0 0.0 0.0 0.0 16 0.0053004243994984705 0.0 0.0 0.0 0.0 17 0.0060315174201189484 0.0 0.0 0.0 0.0 18 0.006945383695894547 0.0 0.0 0.0 0.0 19 0.007859249971670146 0.0 0.0 0.0 0.0 20 0.009869755778376461 0.0 0.0 0.0 0.0 21 0.011331941819617419 0.0 0.0 0.0 0.0 22 0.012245808095393017 0.0 0.0 0.0 0.0 23 0.013707994136633974 0.0 0.0 0.0 0.0 24 0.014804633667564692 0.0 0.0 0.0 0.0 25 0.015170180177874932 0.0 0.0 0.0 0.0 26 0.01571849994334029 0.0 0.0 0.0 0.0 27 0.016632366219115888 0.0 0.0 0.0 0.0 28 0.018277325515511967 0.0 0.0 0.0 0.0 29 0.019008418536132443 0.0 0.0 0.0 0.0 30 0.020287831322218283 0.0 0.0 0.0 0.0 31 0.02175001736345924 0.0 0.0 0.0 0.0 32 0.024126069680475794 0.0 0.0 0.0 0.0 33 0.024857162701096273 0.0 0.0 0.0 0.0 34 0.026502121997492353 0.0 0.0 0.0 0.0 35 0.028878174314508908 0.0 0.0 0.0 0.0 36 0.030888680121215224 0.0 0.0 0.0 0.0 37 0.0325336394176113 0.0 0.0 0.0 0.0 38 0.034361371969162495 0.0 0.0 0.0 0.0 39 0.03600633126555857 0.0 0.0 0.0 0.0 40 0.038382383582575126 0.0 0.0 0.0 0.0 41 0.04112398240990192 0.0 0.0 0.0 0.0 42 0.044231127747538956 0.0 0.0 0.0 0.0 43 0.04624163355424527 0.0 0.0 0.0 0.0 44 0.04861768587126183 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAATCG 20 3.9095385E-4 50.687134 50 CGAAATT 15 0.007991793 50.677704 49 AATCGTA 15 0.008277633 50.229187 43 CCACGGT 15 0.008310865 50.1783 40 TATACTA 20 4.1767923E-4 50.012486 30 TACGCGC 15 0.008490803 49.907116 24 CCAATCA 15 0.008512341 49.875137 23 ATATACG 15 0.008540095 49.83408 21 TTAGTAA 20 4.2510947E-4 49.83408 20 AGACTAC 15 0.008567915 49.79309 15 ATTACGC 15 0.008567915 49.79309 10 TACGGTA 25 2.1378222E-5 49.79309 4 TCCGTAT 15 0.008567915 49.79309 17 TATACGA 200 0.0 49.79309 2 TATACGG 54535 0.0 49.40961 2 TACGGGA 12915 0.0 49.407543 4 GTATACG 54765 0.0 49.402134 1 ATACGGG 53705 0.0 49.399048 3 TACGGGG 33795 0.0 49.351074 4 TACGGGT 2700 0.0 49.332043 4 >>END_MODULE