FastQCFastQC Report
Fri 17 Jun 2016
SRR1526698_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526698_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171641
Sequences flagged as poor quality0
Sequence length64
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT9570.5575590913592906No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.423558473791227No Hit
GAATTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5870.34199288048892745No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4580.26683601237466575No Hit
GAATTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.25809684166370506No Hit
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3850.22430538158132382No Hit
GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.18934869873748114No Hit
GAATTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.18760086459528902No Hit
GAATTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.1870182532145583No Hit
GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.11827011028833437No Hit
GAATTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.10720049405445087No Hit
GAATTAGGGGAGTGCAGTGCTGAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.10545265991225873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCAT1450.058.49484358
GGATCTA150.004530847558.47760857
TTAGGTC150.00464828958.1009944
TAGGTCA150.00464828958.1009945
TACCATA201.9998763E-458.0330442
ACCTAGG150.004669883358.0330451
TATAGCT303.7279642E-758.0330453
AATCGGG201.9998763E-458.0330453
TACCTAG150.00467529458.01607550
AGCCTTA150.00467529458.01607546
AGGCTAG258.639001E-657.99912637
CCTTACC150.004680708557.99912348
GCCTTAC150.004680708557.99912347
GCACAAT150.004680708557.99912348
TGCTACT407.1304385E-1057.99912338
CTTACCC150.004680708557.99912349
TATGCAT150.00469698157.94832233
TGATCTA150.00469698157.94832232
CGGAACC150.00470241557.93140426
TTACTTG150.00470241557.93140413
Produced by FastQC (version 0.11.5)